We narrowed to 5,756 results for: cki

1. Ecoli cpGFP

   Plasmid

   #215713

   Purpose

   Expresses cpGFP in Ecoli

   Depositor

   Katharine Diehl

   Article

   Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

   Insert

   cpGFP
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   circularly permuted GFP

   Promoter

   T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSEED-mGreenLantern:ALFA

   Plasmid

   #219644

   Purpose

   Permits GoldenGate assembly of donor plasmids (BsmBI) to knock-in mScarlet:ALFA using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   mGreenLantern:ALFA SEED Cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Flag-TurboID-NES_pLX304

   Plasmid

   #202008

   Purpose

   expresses TurboID in the mammalian cytosol, lentiviral vector

   Depositor

   Alice Ting

   Article

   Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

   Insert

   Flag-TurboID-NES
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-BbsI-PITCh

   Plasmid

   #127875

   Purpose

   For user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assembly

   Depositor

   Peter Kaiser

   Article

   Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRRL sEF1a HA.NLS.Sce(opt).T2A.IFP

   Plasmid

   #31484

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   EF1s HA NLS Sce(opt) T2A IFP1.4
   

   Use

   Lentiviral

   Tags

   HA and NLS

   Expression

   Mutation

   "Opt" indicates the I-SceI coding seque…

   Promoter

   Available since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. LeGO-EBFP2

   Plasmid

   #85213

   Purpose

   Lentiviral vector expressing EBFP2 fluorescent protein driven by SFFV promoter

   Depositor

   Boris Fehse

   Article

   Mohme et al Mol Ther. 2017 Jan 18. pii: S1525-0016(1

   Insert

   EBFP2
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Dec. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSEED

   Plasmid

   #219658

   Purpose

   Permits GoldenGate assembly of new SEED cassettes via Golden Gate assembly (BsaI)

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   Empty SEED Cassette (3xP3-dsRed)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFETCh_Donor (EMM0021)

   Plasmid

   #63934

   Purpose

   3X Flag with P2A-Neo for 3' tagging of endogenous proteins

   Depositor

   Richard M. Myers, Eric Mendenhall

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   Glycine-Serine-3XFlag-P2A-NeoR

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. mini-donor - SOX17-EGFP

   Plasmid

   #206048

   Purpose

   this mini-donor knockin vector can be used to knock EGFP into SOX17 locus

   Depositor

   Xiaojun Lian

   Article

   Haideri et al Stem Cell Reports. 2024 May 14;19(5):744

   Insert

   nuclear EGFP
   

   Use

   Knockin donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSEED-sfGFP

    Plasmid

    #219639

    Purpose

    Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    sfGFP SEED Cassette
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. LeGO-mOrange2

    Plasmid

    #85212

    Purpose

    Lentiviral vector expressing mOrange2 fluorescent protein driven by SFFV promoter

    Depositor

    Boris Fehse

    Article

    Mohme et al Mol Ther. 2017 Jan 18. pii: S1525-0016(1

    Insert

    mOrange2
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-BI

    Plasmid

    #126475

    Purpose

    High-copy number vector for the co-expression of two genes in mammalian cells

    Depositor

    Bill Hansson

    Article

    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. V5-GFP-APEX2-NES_pLX208

    Plasmid

    #202018

    Purpose

    expresses GFP-APEX2 in the mammalian cytosol, lentiviral vector

    Depositor

    Alice Ting

    Article

    Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

    Insert

    V5-GFP-APEX2-NES
    

    Use

    Lentiviral

    Tags

    GFP and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. mTagRFP-T-Endosomes-14

    Plasmid

    #58014

    Purpose

    Localization: Endosomes, Excitation: 555, Emission: 584

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Endosomes (RHOB Human)
    

    Use

    Tags

    mTagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

15. APEX2-csChBP

    Plasmid

    #108876

    Purpose

    Expression of APEX2 fused to a conditionally stable nanobody against mCherry

    Depositor

    Rob Parton

    Article

    Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

    Insert

    APEX2-csChBP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKD013

    Plasmid

    #207931

    Purpose

    Premade plasma membrane marker, pUBQ10:PiP1;4:mTurquoise2:tHSP18.2M, for braiding

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. PGK mScarlet-Synaptophysin

    Plasmid

    #206145

    Purpose

    PGK mScarlet-Synaptophysin

    Depositor

    Erika Holzbaur

    Article

    Dou et al J Cell Biol. 2024 Jun 3;223(6):e20230709

    Insert

    synaptophysin (Syp Rat)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-OSF-PP

    Plasmid

    #154247

    Purpose

    backbone with PreScission protease-cleavable N-terminal OneStrepFLAG tag

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

    Type

    Empty backbone

    Use

    Tags

    FLAG and Strep-Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRS413-CUP1pr

    Plasmid

    #221109

    Purpose

    Copper-inducible gene expression

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. V5-GFP-TurboID-KDEL_pLX208

    Plasmid

    #202020

    Purpose

    expresses GFP-TurboID in the mammalian ER lumen, lentiviral vector

    Depositor

    Alice Ting

    Article

    Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

    Insert

    V5-TurboID-KDEL
    

    Use

    Lentiviral

    Tags

    GFP and V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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