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HoxD del control ds

Plasmid

#131336

Purpose

gRNA to delete control region near HoxD. Use with HoxD del control us

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

CTRL-gRNA-C-del

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only
HoxD del us1

Plasmid

#131337

Purpose

gRNA to delete a nucleation site near HoxD. Use with HoxD del ds1

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

EVX2-gRNA-N-del

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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HoxD del control us

Plasmid

#131335

Purpose

gRNA to delete control region near HoxD. Use with HoxD del control ds

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

CTRL-gRNA-N-del

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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EZH2-Promoter-gRNA

Plasmid

#131329

Purpose

gRNA to generate endogenously tagged -tetR-EZH2

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

EZH2-Promoter-gRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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EED-F97A

Plasmid

#131326

Purpose

gRNA to generate F97A cage-mutant EED

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

EED-KO-gRNA-2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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EED-KO-gRNA-1

Plasmid

#131321

Purpose

gRNA to knockout EED. Use with EED-KO-gRNA-2

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

EED-KO-gRNA-1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only
ORF18(R158A_L159A)-3xFLAG pCDNA4

Plasmid

#129814

Purpose

Expresses KSHV ORF18 (R158A_L159A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Arginine 158 to Alanine and Leucine 159 t…

Promoter

CMV

Available since

Oct. 23, 2019

Availability

Academic Institutions and Nonprofits only

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K8.1Pr-pGL4.16-OriLyt

Plasmid

#131038

Purpose

Firefly Luciferase reporter for K8.1 promoter from KSHV with the lytic origin of replication

Depositor

Britt Glaunsinger

Article

Nandakumar et al PLoS Pathog. 2019 May 16;15(5):e1007774.

Insert

KSHV Lytic Origin of replication

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18(G228A)-3xFLAG pCDNA4

Plasmid

#129809

Purpose

Expresses KSHV ORF18 (G228A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Glycine 228 to Alanine

Promoter

CMV

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18(L29A)-3xFLAG pCDNA4

Plasmid

#129777

Purpose

Expresses KSHV ORF18 (L29A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Leucine 29 to Alanine

Promoter

CMV

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18(L37A)-3xFLAG pCDNA4

Plasmid

#129779

Purpose

Expresses KSVH ORF18 (L37A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Leucine 37 to Alanine

Promoter

CMV

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18(K75A)-3xFLAG pCDNA4

Plasmid

#129784

Purpose

Expresses KSHV ORF18 (K75A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Lysine 75 to Alanine

Promoter

CMV

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18(G65A)-3xFLAG pCDNA4

Plasmid

#129781

Purpose

Expresses KSHV ORF18 (G65A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Glycine 65 to Alanine

Promoter

CMV

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18(L107A)-3xFLAG pCDNA4

Plasmid

#129787

Purpose

Expresses KSHV ORF18 (L107A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Leucine 107 to Alanine

Promoter

CMV

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18(W170)-3xFLAG pCDNA4

Plasmid

#129803

Purpose

Expresses KSHV ORF18 (W170A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Tryptophan 170 to Alanine

Promoter

CMV

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18(G152A)-3xFLAG pCDNA4

Plasmid

#129800

Purpose

Expresses KSHV ORF18 (G152A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Glycine 152 to Alanine

Promoter

CMV

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18(L151A)-3xFLAG pCDNA4

Plasmid

#129799

Purpose

Expresses KSHV ORF18 (L151A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Leucine 151 to Alanine

Promoter

CMV

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18(L151A_G152A)-3xFLAG pCDNA4

Plasmid

#129813

Purpose

Expresses KSHV ORF18 (L151A_G152A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Leucine 151 to Alanine and Glycine 152 to…

Promoter

CMV

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18(L191A)-3xFLAG pCDNA4

Plasmid

#129807

Purpose

Expresses KSHV ORF18 (L191A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Leucine 191 to Alanine

Promoter

CMV

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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ORF18(E36A_L37A)-3xFLAG pCDNA4

Plasmid

#129810

Purpose

Expresses KSHV ORF18 (E36A_L37A) with Cterm 3xFLAG tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Glutamic acid 37 to Alanine and Leucine 3…

Promoter

CMV

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge