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We narrowed to 4,045 results for: golden gate

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Showing: 1521 - 1540 of 4045 results

  1. IBMc050

    Plasmid
    #161938
    Purpose
    Carries Golden Gate substitution insert for IBM with M86 intein. Plux2 as C-lobe promoter
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB1A3-BbsI-M86N-cat-Plux2-B32-M86C-SapI
    Expression
    Bacterial
    Promoter
    Plux2
    Available Since
    March 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    161938-plasmid-map-sequence-id-327548

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  4. pRDB_248

    Plasmid
    #245335
    Purpose
    Cas12a CRISPRko positive control guide; targets CD63
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD63 (CD63 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245335-plasmid-map-sequence-id-488021

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  5. pAGM4723:Ble:Cas9

    Plasmid
    #112208
    Purpose
    Golden Gate Level 2 construct encoding Cas9-YFP, Zeocin resistance.
    Depositor
    Thomas Mock
    Inserts
    Cas9
    SheBle
    Use
    CRISPR; Diatom, phaeodactylum
    Tags
    Phaeodactylum fcp promoter and terminator and YFP
    Mutation
    Phaeodactylum Fcp promoter
    Promoter
    Phaeodactylum Fcp
    Available Since
    July 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    112208-plasmid-map-sequence-id-217248

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  8. pMV_AA101

    Plasmid
    #245323
    Purpose
    Cas9 CRISPRa positive control guide; targets CD274
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD274 (CD274 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245323-plasmid-map-sequence-id-488047

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  9. pRDB_510

    Plasmid
    #245337
    Purpose
    Cas9 CBE positive control guide; targets CD274
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD274 (CD274 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245337-plasmid-map-sequence-id-488041

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  10. pRDB_509

    Plasmid
    #245336
    Purpose
    Cas9 ABE positive control guide; targets CD274
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD274 (CD274 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245336-plasmid-map-sequence-id-488033

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  11. pTwist-Kan-HC-L0-TaMOFB1(int)

    Plasmid
    #242005
    Purpose
    L0 CDS1 Golden Gate part of the pre-mRNA (excluding UTRs) of Triticum aestivum MORE FLORET 1 (MOF-1) / MULTI-FLORET SPIKELET 2 (MFS-2) B-subgenome homoeolog (TraesCS2B02G420900)
    Depositor
    Cristobal Uauy
    Insert
    TaMOF-B1_with_introns
    Use
    Synthetic Biology
    Expression
    Plant
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    242005-plasmid-map-sequence-id-487883

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  12. pAIP4_3CX2

    Plasmid
    #234221
    Purpose
    Heterologous protein expression of myosin-binding protein C, cardiac-type in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3CX2 (MYBPC3 Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234221-plasmid-map-sequence-id-464186

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  13. pAIP4_4ACJ

    Plasmid
    #234212
    Purpose
    Heterologous protein expression of Wufb25h12 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4ACJ (oxr1a Zebrafish)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234212-plasmid-map-sequence-id-464177

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  14. pAIP4_1EYH

    Plasmid
    #234159
    Purpose
    Heterologous protein expression of epsin-1 (EPS-15-interacting protein 1) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1EYH (Epn1 Rat)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234159-plasmid-map-sequence-id-464125

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  15. MUT_S1-spacer-H1

    Plasmid
    #218399
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron following a neutral spacer, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Expression
    Mammalian
    Promoter
    tetracycline-responsive promoter
    Available Since
    June 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    218399-plasmid-map-sequence-id-436404

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  16. HS_NAM-B1

    Plasmid
    #154064
    Purpose
    Level M, P1 Golden Gate vector. OsAct::Hyg-nosT/HvHSP17::Cre-hspT/ZmUbi::loxP-GUS-loxP-NAM-B1-nosT
    Depositor
    Cristobal Uauy
    Article
    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:
    Inserts
    Hygromycin phosphotransferase
    Cre recombinase
    LoxP-flanked GUS and TtNAM-B1
    Use
    Cre/Lox and Synthetic Biology; Golden gate
    Expression
    Bacterial and Plant
    Mutation
    The TtNAM-B1 gene sequence was domesticated to re…
    Promoter
    HvHSP17, OsAct, and ZmUbi
    Available Since
    Sept. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    154064-plasmid-map-sequence-id-313309

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  18. IBMc460

    Plasmid
    #161940
    Purpose
    Carries Golden Gate substitution insert for IBM with gp41-1 intein. PPhlF as C-lobe promoter
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB1A3-BbsI-GY-gp-41-1N-cat-PPhlF-RiboJ-B32-gp41-1C-SS-SapI
    Expression
    Bacterial
    Promoter
    PPhlF
    Available Since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    161940-plasmid-map-sequence-id-329041

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  19. IBMc180

    Plasmid
    #161942
    Purpose
    Carries Golden Gate substitution insert for acVHH-assisted bisection mapping
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB1A3-BbsI-10aa-acVHH-T-cat-PBAD(SapI)-RiboJ-BCD22-acVHH-10aa-SapI
    Expression
    Bacterial
    Promoter
    PBAD
    Available Since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    161942-plasmid-map-sequence-id-329042

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  20. pAIP4_3RZY

    Plasmid
    #234156
    Purpose
    Heterologous protein expression of fatty acid-binding protein, adipocyte (ALBP) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3RZY (FABP4 Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234156-plasmid-map-sequence-id-464122

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Showing: 1521 - 1540 of 4045 results