We narrowed to 8,596 results for: Set

1. pLV-Hygro-EF1A-PBM-Linker-eGFP

   Plasmid

   #176069

   Purpose

   EGFP fused to the C-terminus of a PBM domain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   PBM
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBetaActin-FKBP-mScarlet-KIFC1motor

   Plasmid

   #191334

   Purpose

   Expresses the fluorescently labeled minus-end directed KIFC1 motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

   Depositor

   Frank Bradke

   Article

   Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

   Insert

   FKBP-mScarlet-KIFC1 (KIFC1 Human)
   

   Expression

   Mammalian

   Mutation

   motor domain aa 125-673; N593K

   Available Since

   Jan. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCC_01 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRE

   Plasmid

   #139086

   Purpose

   Expresses human codon-optimized SpCas9 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   SpCas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTMiniTrex-mCherry-Shark2

   Plasmid

   #233144

   Purpose

   Serves as a template plasmid for amplifying Shark2 (Tet-ON) hammerhead aptazyme-3xTy-tagging cassette

   Depositor

   Ronald Etheridge

   Article

   Wiedeman et al Proc Natl Acad Sci U S A. 2025 Feb 25;12

   Inserts

   mCherry
   Shark2
   

   Use

   Prokaryotic expression

   Tags

   3x Ty tag

   Mutation

   Codon optimized for Trypanosoma cruzi

   Available Since

   March 20, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pLC-HA active Nedd8-P2A-Hygro

   Plasmid

   #124307

   Purpose

   Lentiviral vector for expression of HA tagged active or C-terminal cleaved Nedd8-P2A-Hygro casette from a CMV promoter

   Depositor

   Eva Gottwein

   Article

   Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

   Insert

   Nedd8 (NEDD8 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Five C-terminal amino acids missing compared to w…

   Promoter

   CMV

   Available Since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAVS1-PdTK-CAG-mCh-[uBglII]

   Plasmid

   #107280

   Purpose

   puro-deltaTK gene-trap selection cassette (AAVS1 donor vector backbone) with constitutive mCherry

   Depositor

   Knut Woltjen

   Article

   Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

   Insert

   AAVS1 puro-deltaTK; CAG-mCherry (PPP1R12C )
   

   Available Since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF2048

   Plasmid

   #143332

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   PRDM9 (PRDM9 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. sgRNA1_ASCL1

   Plasmid

   #64129

   Purpose

   Photoactivatable transcription system. Lentiviral expression of ASCL1 sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette.

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA1_ASCL1 (ASCL1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLC-CUL4A WT-P2A-Puro

   Plasmid

   #124304

   Purpose

   Lentiviral vector for expression of wild-type CUL4A-P2A-Puro casette from a CMV promoter

   Depositor

   Eva Gottwein

   Article

   Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

   Insert

   CUL4A (CUL4A Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. AA145

    Plasmid

    #215937

    Purpose

    Fragmid fragment: (guide cassette) guide expression; Chen F+E trRNA (preferred)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v4 [Sp]
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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