We narrowed to 2,383 results for: LRE

1. pJRH029

   Plasmid

   #171060

   Purpose

   Plasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in between

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Insert

   Gag-spyCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   2x SV40 NLS and 3x FLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAVS1-TetOn-dCas9-KRAB

   Plasmid

   #115545

   Purpose

   Doxycycline inducible dCas9-KRAB targeting construct for AAVS1 locus

   Depositor

   Rene Maehr

   Article

   Genga et al Cell Rep. 2019 Apr 16;27(3):708-718.e10.

   Type

   Empty backbone

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMK287 (mAID-Hygro)

   Plasmid

   #72825

   Purpose

   C-terminal tagging with mAID using CRISPR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mAID-Hygro
   

   Use

   CRISPR

   Tags

   mAID

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19_CRISPR_DpmrA

   Plasmid

   #160903

   Purpose

   CRISPR_Cas9, lambda red recombineering

   Depositor

   Anne-Catrin Uhlemann

   Article

   McConville et al Cell Rep. 2020 Oct 27;33(4):108313. doi:

   Inserts

   Cas9
   Lambda Red Recombineering Genes
   Zeocin Resistance Cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   araBAD

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-PD-1-SNAP

   Plasmid

   #223576

   Purpose

   Lentiviral expression of human PD-1 with SNAP tag in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

   Insert

   PD-1 (PDCD1 Human)
   

   Use

   Lentiviral; mammalian expression

   Tags

   SNAP

   Expression

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAGM55261

   Plasmid

   #153210

   Purpose

   Level 2 cloning vector for a single guide RNA, already contains Cas9, Bar selection cassette and FAST marker

   Depositor

   Sylvestre Marillonnet

   Article

   Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. eSpCas9(1.1)_No_FLAG_AAVS1_T2

   Plasmid

   #79888

   Purpose

   Expresses the AAVS1 T2 gRNA in combination with FLAGless eSpCas9(1.1) to target the AAVS1 "safe harbor" locus.

   Depositor

   Yannick Doyon

   Article

   Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Untagged eSpCas9(1.1)

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. 3xFLAG-Halo-Dyskerin

   Plasmid

   #207578

   Purpose

   CRISPR-Cas9 genome editing encoding a FLAG-HaloTag at the endogenous dyskerin locus

   Depositor

   Jens Schmidt

   Article

   Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

   Insert

   HaloTag DKC1
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. EGFR-FR

   Plasmid

   #179263

   Purpose

   Human EGFR C-terminally labeled with FusionRed

   Depositor

   Jared Toettcher

   Article

   Farahani et al Cell Rep. 2021 Dec 28;37(13):110181. doi

   Insert

   EGFR (EGFR Human)
   

   Use

   Lentiviral

   Tags

   FusionRed

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Feb. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAVS1_Puro_PGK1_3xFLAG_Twin_Strep

    Plasmid

    #68375

    Purpose

    Doyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging.

    Depositor

    Yannick Doyon

    Article

    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and TALEN; Zfn

    Tags

    3xFLAG_Twin_Strep

    Expression

    Mammalian

    Mutation

    Promoter

    human PGK1

    Available since

    Oct. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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