We narrowed to 8,420 results for: construct

1. pMECOM.1.0-gDNA

   Plasmid

   #132445

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   MECOM (MECOM Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKLF10.1.0-gDNA

   Plasmid

   #132433

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   KLF10 (KLF10 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJK103

   Plasmid

   #73265

   Purpose

   Produces Acetobacter aceti 1023 hypothetical acetic acid resistance protein B, C-terminal His6 tag (AaAarBH6)

   Depositor

   T. J. Kappock

   Insert

   hypothetical acetic acid resistance protein B
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJK540

   Plasmid

   #72446

   Purpose

   Produces Acetobacter aceti 1023 small hypothetical protein with N-terminal His6 tag (H6AaOrfX); N5-carboxyaminoimidazole ribonucleotide mutase (AaPurE1); NCAIR synthetase (AaPurK)

   Depositor

   T. J. Kappock

   Inserts

   hypothetical protein
   N5-carboxyaminoimidazole ribonucleotide mutase
   N5-carboxyaminoimidazole ribonucleotide synthetase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7 and n/a

   Available Since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJK552

   Plasmid

   #72451

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with inserted Val2 (AaPurE1-V2ins); N5-carboxyaminoimidazole ribonucleotide synthetase, Val319>Phe mutant (AaPurK-V319F)

   Depositor

   T. J. Kappock

   Inserts

   N5-carboxyaminoimidazole ribonucleotide mutase
   N5-carboxyaminoimidazole ribonucleotide synthetase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Val319 to Phe and inserted Val2

   Promoter

   T7

   Available Since

   March 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJK141

   Plasmid

   #73316

   Purpose

   Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with Pas2p signal sequence, c-myc tag, and His6-tag

   Depositor

   T. J. Kappock

   Insert

   delta-12 fatty acid acetylenase
   

   Use

   Tags

   His6, Pas2p signal sequence, and c-myc

   Expression

   Yeast

   Mutation

   synthetic gene

   Promoter

   AOX1

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJK586

   Plasmid

   #72459

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Ser57>Thr/Trp165>Phe mutant (AaPurE1fwf-S57T)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Mutation changes tryptophan-34 to phenylalanine, …

   Promoter

   T7

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJK565

   Plasmid

   #72454

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/His59>Ala/Trp165>Phe mutant (AaPurE1fwf-H59A)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Mutation changes tryptophan-34 to phenylalanine, …

   Promoter

   T7

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJK578

   Plasmid

   #72457

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Trp34>Phe/Ser57>Ala/Trp165>Phe mutant (AaPurE1fwf-S57A)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Mutation changes tryptophan-34 to phenylalanine, …

   Promoter

   T7

   Available Since

   Feb. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJK569

    Plasmid

    #72455

    Purpose

    Produces Franciscella tularensis N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal TEV protease-cleavable His6 tag (H6tFtPurE1), synthetic gene

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    synthetic gene with ~51 silent substitutions

    Promoter

    T7

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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