We narrowed to 2,565 results for: organization Addgene

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aTY178_pUAS_Dkk1-IRES-tagBFP2_WPRE

Plasmid

#225353

Purpose

Lentiviral vector for DKK1 and BFP expression

Depositor

Wendell Lim

Article

Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

Insert

Dkk1 (Dkk1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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aTY135_mtk02_pCAG-nfGFPtether_P2A_H2B-IRFP_SV40pA

Plasmid

#225350

Purpose

piggyback backbone for non-fluorescent GFP tether and H2B-IRFP expression

Depositor

Wendell Lim

Article

Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

Insert

H2B (H2B Synthetic, Human)

Tags

miRFP and non-fluorescent GFP (Y67F)-P2A

Expression

Mammalian

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-Ctr1-gRNA-EGFP

Plasmid

#226000

Purpose

Negative control for CRISPRi-KD

Depositor

Miguel Ramalho-Santos

Article

Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

Insert

negtive control sgRNA of no specific targets in human genome

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-Ctr2-gRNA-EGFP

Plasmid

#226001

Purpose

Negative control for CRISPRi-KD

Depositor

Miguel Ramalho-Santos

Article

Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

Insert

negtive control sgRNA of no specific targets in human genome

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-hL1-gRNA1-EGFP

Plasmid

#226002

Purpose

CRISPRi-KD of human LINE1

Depositor

Miguel Ramalho-Santos

Article

Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

Insert

sgRNA targeting human L1HS

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGL3-hL1-gRNA2-EGFP

Plasmid

#226003

Purpose

CRISPRi-KD of human LINE1

Depositor

Miguel Ramalho-Santos

Article

Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

Insert

sgRNA targeting human L1HS

Expression

Mammalian

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5 Myc-tagged PSK (K57A)

Plasmid

#197150

Purpose

Expression of kinase-deficient MYC-tagged PSK1-alpha (K57A) / TAOK2 (K57A) in mammalian cells

Depositor

Jonathan Morris

Article

Moore et al J Biol Chem. 2000 Feb 11;275(6):4311-22.

Insert

TAOK2 (TAOK2 Human)

Tags

Myc

Expression

Mammalian

Mutation

Changed K 57 to A

Promoter

SV40

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pST_CAHS1_PARRC_150-189 (pBS0658)

Plasmid

#185201

Purpose

For the mammalian expression of the tardigrade protein CAHS1_PARRC_150-189. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CAHS1_PARRC_150-189

Expression

Mammalian

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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PvLEA4_repeats_k3_26_mCh-SspB (pBS1075)

Plasmid

#185299

Purpose

Mammalian expression of sleeping chironomid protein PvLEA4_repeats_k3_26 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation.

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_shuffle_3

Expression

Mammalian

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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DHN_VrDHN1a_mCh-SspB (pBS1074)

Plasmid

#185298

Purpose

Mammalian expression of riverbank grape plant protein DHN_VrDHN1a attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation.

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

PvLEA4_repeats_k3_26

Expression

Mammalian

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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