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pAWp9-2

Plasmid

#73853

Purpose

pFUGW-UBCp-RFP-CMVp-GFP-U6p-RFP-sg1

Depositor

Timothy Lu

Article

Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

Insert

UBCp-RFP-CMVp-GFP-U6p-RFP-sg1

Use

Lentiviral

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pGC461

Plasmid

#71363

Purpose

distal tip cell expression of DAF-16 in C. elegans

Depositor

Jane Hubbard

Article

Michaelson et al Development. 2010 Feb;137(4):671-80. doi

Insert

daf-16 (daf-16 Nematode)

Tags

GFP(65C)

Expression

Worm

Promoter

Blelloch et al., 1999; Mathies et al., 2003

Available Since

Dec. 8, 2015

Availability

Academic Institutions and Nonprofits only

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phRL-TKlk-lin-41_LCS-wt

Plasmid

#26542

Depositor

Chang-Zheng Chen

Article

Trujillo et al EMBO J. 2010 Oct 6;29(19):3272-85. doi:

Insert

lin-41_LCS-wt

Expression

Mammalian

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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pDP-let-7_LPA

Plasmid

#26559

Depositor

Chang-Zheng Chen

Article

Trujillo et al EMBO J. 2010 Oct 6;29(19):3272-85. doi:

Insert

cel-pre-let-7_LPA

Use

Transcription vector

Mutation

loop mutant A

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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pDP-let-7_LPC

Plasmid

#26561

Depositor

Chang-Zheng Chen

Article

Trujillo et al EMBO J. 2010 Oct 6;29(19):3272-85. doi:

Insert

cel-pre-let-7_LPC

Use

Transcription vector

Mutation

loop mutant C

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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pDP-let-7_LPD

Plasmid

#26562

Depositor

Chang-Zheng Chen

Article

Trujillo et al EMBO J. 2010 Oct 6;29(19):3272-85. doi:

Insert

cel-pre-let-7_LPD

Use

Transcription vector

Mutation

loop mutant D

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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ced-4S in pXHA

Plasmid

#134970

Purpose

ced-4S cDNA cloned into pXHA backbone, used for production of antibodies

Depositor

Robert Horvitz

Article

Chen et al Science. 2000 Feb 25;287(5457):1485-9. d

Insert

ced-4 (ced-4 Nematode)

Availability

Academic Institutions and Nonprofits only

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pS241

Plasmid

#139645

Purpose

full length ced-4L cDNA

Depositor

Robert Horvitz

Article

Shaham et al Cell. 1996 Jul 26;86(2):201-8. doi: 10.1

Insert

ced-4 (ced-4 Nematode)

Use

Cloning vector

Expression

Bacterial

Mutation

addition of K212 and R213 compared to NCBI refere…

Availability

Academic Institutions and Nonprofits only

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pSKI

Plasmid

#232484

Purpose

pSKI can be used for single-copy transgene expression within the SKI PLACE System or for extrachromosomal arrays. It contains an MCS with 47 single cuts and two synthetic 900 bp homology arms.

Depositor

Carlos Silva García

Article

Dinneen et al G3 (Bethesda). 2025 Sep 19:jkaf220. doi:

Insert

pSKI

Expression

Worm

Available Since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

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pAW12.lentiguide.GFP

Plasmid

#104374

Purpose

GFP vector for cloning in guides

Depositor

Richard Young

Article

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pAS3-rab-3P::AI::ChR2(H134R)

Plasmid

#232601

Purpose

Pan-neuronal expression of opsin 'ChR2(H134R)', regulated by rab-3 promoter and unc-54 3' UTR

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

Channelrhodopsin2

Expression

Worm

Mutation

H134R

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pLJ57 (Pmec-17_GCaMP8f_SL2_mKate2_let-858_3'UTR)

Plasmid

#239865

Purpose

expression of GCaMP8f in C. elegans under mec-17-promoter control

Depositor

Monika Scholz

Article

Liu et al Genetics. 2024 Oct 7;228(2):iyae125. doi

Inserts

GCaMP8f
mKate2

Tags

6xHis

Expression

Worm

Mutation

codon optimized with introns for C. elegans

Promoter

mec-17

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pDD104 (Peft-3::Cre)

Plasmid

#47551

Purpose

Cre recombinase expression plasmid for removal of floxed selectable markers in the C. elegans germline

Depositor

Bob Goldstein

Article

Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

Insert

Cre Recombinase

Use

Cre/Lox

Expression

Worm

Promoter

eef-1A.1 (eft-3)

Available Since

Sept. 9, 2013

Availability

Academic Institutions and Nonprofits only

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pCFJ782 - HygroR

Plasmid

#190933

Purpose

Hygromycin resistance co-injection marker

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

Hygromycin resistance gene

Expression

Worm

Promoter

rps-0

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLAG-YY1

Plasmid

#104396

Purpose

FLAG-YY1

Depositor

Richard Young

Article

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Insert

YY1

Tags

FLAG

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCFJ601 - Peft-3 Mos1 transposase

Plasmid

#34874

Purpose

Mos1 transposase expression vector for use in creating targeted transgene insertions and targeted deletions in C. elegans

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

Mos1 transpoase

Use

Worm targeting

Promoter

eef-1A.1 (eft-3)

Available Since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pHIP_RNA1

Plasmid

#61259

Purpose

Orsay virus RNA1 genome segment expression plasmid for virus reverse genetics

Depositor

David Wang

Article

Jiang et al J Virol. 2014 Oct 15;88(20):11774-81. do

Insert

Orsay RNA1

Expression

Worm

Promoter

heat shock promoter

Available Since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pHIP_RNA2

Plasmid

#61260

Purpose

Orsay virus RNA2 genome segment expression plasmid for virus reverse genetics

Depositor

David Wang

Article

Jiang et al J Virol. 2014 Oct 15;88(20):11774-81. do

Insert

Orsay RNA2

Expression

Worm

Promoter

heat shock promoter

Available Since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAW13.lentiguide.mCherry

Plasmid

#104375

Purpose

mCherry vector for cloning in guides

Depositor

Richard Young

Article

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pGFPmAID

Plasmid

#174213

Purpose

GFP::mAID donor backbone

Depositor

Masato Kanemaki

Article

Negishi et al Genetics. 2021 Dec 2. pii: 6448232. doi:

Type

Empty backbone

Expression

Worm

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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