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Plasmid#120353PurposepiggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.DepositorInsertOKMS cassette
UseCRISPR; Piggybac transposonTagsExpressionMammalianMutationKlf4 [10-483]PromoterAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-OKMS-KRAB-dCas9
Plasmid#120356PurposepiggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.DepositorInsertOKMS cassette
UseCRISPR; Piggybac transposonTagsExpressionMammalianMutationKlf4 [10-483]PromoterAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKG139
Plasmid#69755PurposeRetroviral expression of CENP-CDepositorInsertCENP-C (CENPC Human)
UseRetroviralTagsmCherry-TEV-SExpressionMammalianMutationL341F, N570D relative to NP_001803.2 (numbering b…PromoterAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA_RGG-RFP-RGG
Plasmid#124934PurposeExpression of RGG-RFP-RGGDepositorInsertRGG-RFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR_RAF1_94
Plasmid#111826PurposeKnockout Raf1DepositorInsertgRNA targeting RAF1 94-114 (RAF1 Human)
UseCRISPRTagsmCherryExpressionBacterial and MammalianMutationPromoterU6Available sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSin_EF1a_CreN59_nMag_NLS_P2A_mCh_IRES_PuroR
Plasmid#176310PurposeExpresses CreN59-nMag-NLS with mCherry marker.DepositorInsertCreN59_nMag_NLS_P2A_mCherry_IRES_puroR
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman EF1alphaAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRE-BI-aaAFB2
Plasmid#207839PurposeInducible AFB2 Plasmids for rapid depletion of GFP-tagged proteins in mammalian cellsDepositorInsertsatAFB2
mCherry
miniIAA7
VHH-GFP4
UseTags3X FLAG-Tag and weak NLSExpressionMammalianMutationAll K mutated to RPromoterTRE3G BI promoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDZ149
Plasmid#159359PurposeExpresses cinI and mCherry2 (both tagged with ssrA) from the PlLacO1 promoterDepositorInsertcinI
UseSynthetic BiologyTagsExpressionMutationPromoterPlLacO1Available sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6-pegRNA-GG-acceptor
Plasmid#132777PurposePrime editing in mammalian cellsDepositorInsertExchangeable cassette
UseTagsExpressionMammalianMutationSee manuscriptPromoterU6Available sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-double floxed-Gad67-WPRE-HGHpA
Plasmid#184632PurposeExpresses Gad67, driven by the Ef1a promoter, in a Cre-dependent fashionDepositorInsertGad67 (Gad1 Mouse)
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-double floxed-Gad65-WPRE-HGHpA
Plasmid#184631PurposeExpresses Gad65, driven by the Ef1a promoter, in a Cre-dependent fashionDepositorInsertGad65 (Gad2 Mouse)
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-double floxed-Gat1-HA-WPRE-HGHpA
Plasmid#184635PurposeExpresses the GABA membrane transporter Gat1 fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashionDepositorInsertGat1 (Slc6a1 Mouse)
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPS0382
Plasmid#133232PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with constitutive promoter PsNifH (pOGG043), mCherry (EC15071) and T-pharma (pOGG003)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceApril 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pASPIre3
Plasmid#154844PurposeDerivative of pASPIre2 lacking mCherry but containing a silent 150 bp DNA spacer flanked by attB/P sites; main uASPIre plasmid used in this studyDepositorInsertBxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked silent discriminator
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPS0378
Plasmid#133229PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with constitutive promoter pNeo (pOGG001), mCherry (EC15071) and T-pharma (pOGG003)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPS0384
Plasmid#133234PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with NoP (pOGG113), mCherry (EC15071) and T-pharma (pOGG003)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceApril 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPS0380
Plasmid#133230PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with constitutive promoter Rlv3841pnifH (pOGG082), mCherry (EC15071) and T-pharma (pOGG003)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET_RGG-RFP-RGG
Plasmid#124944PurposeExoression of RGG-RFP-RGGDepositorInsertRGG-RFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
UseTagsHis and mCherryExpressionBacterialMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYG026
Plasmid#65328Purposereporter plasmids used in YeastFab work, in which promoters could be inserted just upstream YFP(YEVenus) to drive its expression, also contains an mCherry gene driven by TEF2 promoter.DepositorInsertccdB
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-OK+9MS-Cas9
Plasmid#120354PurposepiggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.DepositorInsertOK+9MS cassette
UseCRISPR; Piggybac transposonTagsExpressionMammalianMutationKlf4 [1-483]PromoterAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only