We narrowed to 2,603 results for: ubiquitin

1. pcDNA-FRT/T0-eGFPnls-53BP1 1220-1631 WT

   Plasmid

   #60814

   Purpose

   mammalian expression vector

   Depositor

   Daniel Durocher

   Article

   Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

   Insert

   53BP1 (TP53BP1 Human)
   

   Use

   Tags

   eGFP-NLS (SV40)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 10, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. GFP-ataxin-3 C14A

   Plasmid

   #89977

   Purpose

   Expresses catalytic inactive GFP-tagged ataxin-3

   Depositor

   Nico Dantuma

   Article

   Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

   Insert

   ataxin-3 (ATXN3 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   changed Cysteine 14 to Alanine

   Promoter

   Available since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5 FRT/TO SF-HERC2 C4762S (ShB-R)

   Plasmid

   #55614

   Purpose

   Dox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP tagged C4762S (catalytically inactive) HERC2 in Flp-In T-Rex cells

   Depositor

   David Chan

   Article

   Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

   Insert

   3xFLAG tagged HERC2 (C4762S) (HERC2 Human)
   

   Use

   Tags

   3xFLAG and STREP

   Expression

   Mammalian

   Mutation

   C4762 mutated to serine to render HERC2 catalytic…

   Promoter

   Available since

   Sept. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-EFS-BirAopt-GSQ-RNF4ca-P2A-BLAST

   Plasmid

   #208047

   Purpose

   Enables constitutive expression of N-terminal BirAopt-fused RNF4ca (catalytically inactive RING mutant); to perform bioE3; selection with blasticidin

   Depositor

   James Sutherland, Rosa Barrio

   Article

   Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

   Insert

   RNF4 (RNF4 Human)
   

   Use

   Lentiviral

   Tags

   BirAopt

   Expression

   Mutation

   Cys>Ala mutations in conserved RING domain

   Promoter

   EFS

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. TRIPZ-bioGEF-SUMO2nc-PURO

   Plasmid

   #208043

   Purpose

   Enables inducible expression of low-affinity Avitag-SUMO2nc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin

   Depositor

   James Sutherland, Rosa Barrio

   Article

   Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

   Insert

   SUMO2nc (SUMO2 Human)
   

   Use

   Lentiviral

   Tags

   Low-affinity Avitag

   Expression

   Mutation

   Q90P Mutation near C-terminus suppresses cleavage…

   Promoter

   tetO/UBC

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. PHAGE-P CMVt N-HA hE6-AP I (AA 366-852)

   Plasmid

   #119315

   Purpose

   lentiviral vector for expression of HA-Tagged E6-AP I AA 366-852 in mammalian cells

   Depositor

   Peter Howley

   Article

   Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

   Insert

   E6-AP I (UBE3A Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Only AA 366-852

   Promoter

   CMV

   Available since

   March 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOPINK-OTUD4 (OTU, aa 22-196)

   Plasmid

   #61413

   Purpose

   Expresses human OTUD4 (OTU domain) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD4 (OTU domain-containing protein 4) (OTUD4 Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-21 and aa 197-1114.

   Promoter

   T7

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSCVpuro-6xHis_SUMO2

   Plasmid

   #164938

   Purpose

   Expresses 6xHis tagged human Small Ubiquitin Like Modifier 2 (SUMO2) wild type cDNA

   Depositor

   Ji Luo

   Article

   Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   Small Ubiquitin Like Modifier 2 (SUMO2 Human)
   

   Use

   Retroviral

   Tags

   6XHIS

   Expression

   Mutation

   Promoter

   PGK promoter

   Available since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. HA-USPL1-C236A-pcDNA3.1

   Plasmid

   #85764

   Purpose

   mammalian expression of full length HA tagged USPL1, catalytic inactive variant C236A

   Depositor

   Frauke Melchior

   Article

   Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

   Insert

   USPL1, full length, catalytic inactive variant variant C236A (USPL1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   catalytic inactive mutant C236A

   Promoter

   CMV

   Available since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. USPL1cat-C236S-pEYFP_C1

    Plasmid

    #85767

    Purpose

    mammalian expression of USPL1 catalytic domain (aa 212-514) as YFP fusion, catalytic inactive variant C236S

    Depositor

    Frauke Melchior

    Article

    Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

    Insert

    USPL1, catalytic domain (aa 212-514), catalytic inactive variant C236S (USPL1 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    catalytic domain (aa 212-514), catalytic inactiv…

    Promoter

    CMV

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. HA-USPL1-C236S-pcDNA3.1

    Plasmid

    #85763

    Purpose

    mammalian expression of full length HA tagged USPL1, catalytic inactive variant C236S

    Depositor

    Frauke Melchior

    Article

    Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

    Insert

    USPL1, full length, catalytic inactive variant variant C236S (USPL1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    catalytic inactive mutant C236S

    Promoter

    CMV

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV10-Flag-Ovtm

    Plasmid

    #51888

    Purpose

    expression vectore in mammalian cells

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

    Insert

    mFbxl3 (Fbxl3 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    ovtm (I364T)

    Promoter

    CMV

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

13. PHAGE-P CMVt N-HA hE6-AP III (AA 1-84)

    Plasmid

    #119319

    Purpose

    lentiviral vector for expression of HA-Tagged E6-AP III AA 1-84 in mammalian cells

    Depositor

    Peter Howley

    Article

    Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

    Insert

    E6-AP III (UBE3A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Only AA 1-84

    Promoter

    CMV

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-ataxin-3 N-t

    Plasmid

    #89980

    Purpose

    Expresses the GFP-tagged N-terminus (Josephin domain) of ataxin-3

    Depositor

    Nico Dantuma

    Article

    Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

    Insert

    ataxin-3 (ATXN3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    construct contains aa 1-182; deleted aa 183-361

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-HA2-Cul4A-deltaROC

    Plasmid

    #20713

    Depositor

    Yue Xiong

    Article

    McCall et al Mol Cell Biol. 2008 Sep . 28(18):5621-33

    Insert

    Cullin 4A (CUL4A Human)
    

    Use

    Tags

    HA2

    Expression

    Mammalian

    Mutation

    Deletion of residues 594 to 612 from N-terminus

    Promoter

    Available since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

16. GFP-ataxin-3 C-t

    Plasmid

    #89981

    Purpose

    Expresses the GFP-tagged C-terminus of ataxin-3

    Depositor

    Nico Dantuma

    Article

    Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

    Insert

    ataxin-3 (ATXN3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-182

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. GP805

    Plasmid

    #164545

    Purpose

    Expresses Smo-FLAG K348, 360, 434, 444, 448, 543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R

    Depositor

    Gregory Pazour

    Article

    Desai et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    Smoothened K348, 360, 434, 444, 448, 543, 565, 568, 569, 575, 579, 628, 671, 676, 677, 678, 681, 682, 683, 684, 717R (Smo Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    All 21 cytoplamic Lysines of Smoothened changed t…

    Promoter

    GgCryD1

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pfast bac cry1myc

    Plasmid

    #51892

    Purpose

    Backbone plasmid is pfastBacHTa from invitrogen

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

    Insert

    cry1 (Cry1 Mouse)
    

    Use

    Tags

    3x Flag and myc

    Expression

    Mutation

    Promoter

    polyhedrin promoter

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pGEX-6P-1 p37 SHPmut

    Plasmid

    #113503

    Purpose

    Bacterial expression of GST-tagged p37 that is p97 binding deficient due to mutations in the SHP box

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    p37 (Ubxn2b Mouse)
    

    Use

    Tags

    GST-PreScission protease site

    Expression

    Bacterial

    Mutation

    F215A/E218A/QKL220-222AAA

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. PHAGE-P CMVt N-HA hE6-AP I (AA 1-500)

    Plasmid

    #119312

    Purpose

    lentiviral vector for expression of HA-Tagged E6-AP I AA 1-500 in mammalian cells

    Depositor

    Peter Howley

    Article

    Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

    Insert

    E6-AP I (UBE3A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Only AA 1-500

    Promoter

    CMV

    Available since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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