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We narrowed to 1,094 results for: gibson assembly

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Showing: 781 - 790 of 1094 results

  1. pHS1625

    Plasmid
    #239834
    Purpose
    OrufIscB-REC E. coli expression for recombinant purification
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038
    Insert
    OrufIscB-REC
    Tags
    14xHis-TwinStrep-bdSUMO
    Expression
    Bacterial
    Mutation
    Insertion of REC domain from NbaCas9-1
    Promoter
    T7
    Available Since
    July 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    239834-plasmid-map-sequence-id-479361

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  3. pETSUMO2_Ideonella-bGSDM-WELQ

    Plasmid
    #222098
    Purpose
    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Ideonella species with a WELQut protease cleavage site for controlled activation.
    Depositor
    Philip Kranzusch
    Article
    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:
    Insert
    Ideonella-bGSDM-WELQ ORF
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    N-terminal methionine deleted and replaced by sin…
    Promoter
    T7
    Available Since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222098-plasmid-map-sequence-id-437096

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  6. pL0_AC-27877

    Plasmid
    #202110
    Purpose
    Level 0 plasmid containing the promoter from gene 27877 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    27877 promoter
    Use
    Synthetic Biology
    Mutation
    Promoter sequence mutated to remove sapI sites fo…
    Available Since
    Aug. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202110-plasmid-map-sequence-id-405522

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  7. pL0_CE-OriT

    Plasmid
    #202150
    Purpose
    Level 0 plasmid containing an origin of transfer sequence from pPtPBR11 required for making plasmids mobilizable for conjugation with CE overhangs used to build level 1 constructs.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    Origin of transfer
    Use
    Synthetic Biology
    Mutation
    OriT sequence mutated to remove sapI sites domest…
    Available Since
    Aug. 25, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202150-plasmid-map-sequence-id-405613

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  8. pL0_AC-Fld

    Plasmid
    #202125
    Purpose
    Level 0 plasmid containing the promoter from gene 23658 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    Fld promoter
    Use
    Synthetic Biology
    Mutation
    Promoter sequence mutated to remove bsaI site for…
    Available Since
    Aug. 24, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202125-plasmid-map-sequence-id-405541

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  9. pL0_AC-54730

    Plasmid
    #202109
    Purpose
    Level 0 plasmid containing the promoter from gene 54730 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    54730 promoter
    Use
    Synthetic Biology
    Mutation
    Promoter sequence mutated to remove bsaI site for…
    Available Since
    Aug. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202109-plasmid-map-sequence-id-405433

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  10. pL0_AC-51183

    Plasmid
    #202108
    Purpose
    Level 0 plasmid containing the promoter from gene 51183 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
    Depositor
    Christopher Dupont
    Article
    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi
    Insert
    51183 promoter
    Use
    Synthetic Biology
    Mutation
    Promoter sequence mutated to remove sapI sites fo…
    Available Since
    Aug. 23, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    202108-plasmid-map-sequence-id-405432

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Showing: 781 - 790 of 1094 results