Addgene

Showing: 1561 - 1580 of 15152 results

Results per page:

Sort results by:

pMM0288

Plasmid

#128976

Purpose

Reporter plasmid, pZF(3BSc)-yEVENUS

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

pZF(3BSc)-yEVENUS

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM0287

Plasmid

#128975

Purpose

Reporter plasmid, pZF(3BS)-yEVENUS

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

pZF(3BS)-yEVENUS

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM0286

Plasmid

#128974

Purpose

Reporter plasmid, pZF(3oBS)-yEVENUS

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

pZF(3oBS)-yEVENUS

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM0619

Plasmid

#128994

Purpose

Spacer cassette plasmid flanked by ConL2 and ConRE

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL2-Spacer-ConRE-AmpR-Col-E1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM0289

Plasmid

#128977

Purpose

Reporter plasmid, pZF(4BSc)-yEVENUS

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

pZF(4BSc)-yEVENUS

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM0285

Plasmid

#128973

Purpose

Reporter plasmid, pZF(1BS)-yEVENUS

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

pZF(1BS)-yEVENUS

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-NFIA1.1

Plasmid

#112698

Purpose

Expression of NFIA1.1 protein for transient transfection assays

Depositor

Richard Gronostajski

Article

Chaudhry et al J Biol Chem. 1999 Mar 12;274(11):7072-81

Insert

Nuclear Factor I A1.1 (Nfia Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV0286

Plasmid

#149333

Purpose

Bacterial expression of SENP_EuB protease

Depositor

Dirk Görlich

Article

Vera Rodriguez et al J Cell Biol. 2019 Jun 3;218(6):2006-2020

Insert

SENP_EuB protease

Use

Tags

His14 and Tev protease cleavage site

Expression

Bacterial

Mutation

Promoter

Available since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCherry-C1 mCherry-NLS

Plasmid

#58476

Purpose

Expresses a nuclear-targeted mCherry control construct, on a CMV reporter

Depositor

Dyche Mullins

Article

Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

Insert

3X NLS with 3X stop codons

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pET22b RNase A

Plasmid

#58903

Purpose

Bacterial expression of bovine pancreatic ribonuclease A

Depositor

Ronald Raines

Article

delCardayre et al Protein Eng. 1995 Mar;8(3):261-73.

Insert

RNase A (RNASE1 Bovine)

Use

Tags

pelB sequence

Expression

Bacterial

Mutation

See Depositor Comments below

Promoter

T7

Available since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pZW1-snoVector

Plasmid

#73174

Purpose

Nuclear expression of RNAs

Depositor

Ling-Ling Chen

Article

Yin et al Nucleic Acids Res. 2015 Jan;43(1):e5. do

Inserts

SNORD116-13 (SNORD116-13 Human)
SNORD116-14 (SNORD116-14 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCherry-C1-RanQ69L

Plasmid

#30309

Depositor

Jay Brenman

Article

Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

Insert

RanQ69L (RAN N/A)

Use

Tags

FLAG-Cherry

Expression

Mammalian

Mutation

Glutamine 69 is changed to Leucine

Promoter

Available since

July 7, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pRFP-NLS

Plasmid

#105296

Purpose

To visualize nuclear dynamics in living cells

Depositor

Kenneth Yamada

Article

Petrie et al Science. 2014 Aug 29;345(6200):1062-5. d

Insert

Rac1 (RAC1 Human)

Use

Tags

RFP

Expression

Mammalian

Mutation

aa encoding 172-192

Promoter

Available since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSP618

Plasmid

#169235

Purpose

Spear-ATAC lentiviral backbone - includes a mU6-sgRNA cassette with flanking Nextera sequencing adapters

Depositor

William Greenleaf

Article

Pierce et al Nat Commun. 2021 May 20;12(1):2969. doi:

Insert

mU6-sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
H2B-mScarlet- hMeikin(1-332)-mNeonGreen

Plasmid

#174714

Purpose

Stable expression of fluorescent Separase cleavage sensor with human Meikin

Depositor

Iain Cheeseman

Article

Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

Insert

Meikin

Use

Retroviral

Tags

H2B-mScarlet and mNeonGreen

Expression

Mammalian

Mutation

1-332

Promoter

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV0679

Plasmid

#149689

Purpose

Bacterial expression of SENP_EuH protease

Depositor

Dirk Görlich

Article

Vera Rodriguez et al J Cell Biol. 2019 Jun 3;218(6):2006-2020

Insert

SENP_EuH protease

Use

Tags

His14-tag and Tev protease cleavage site

Expression

Bacterial

Mutation

Promoter

Available since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
hMSH2 Clone E3 (full-length)

Plasmid

#16453

Depositor

Bert Vogelstein

Article

Leach et al Cell. 1993 Dec 17. 75(6):1215-25.

Insert

MSH2 (MSH2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination

Plasmid

#40132

Purpose

Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (ELD).

Depositor

Pawel Pelczar

Article

Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

Type

Empty backbone

Use

Tags

FokI nuclease (ELD)

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 31, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination

Plasmid

#40131

Purpose

Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses heterodimeric FokI domain (KKR).

Depositor

Pawel Pelczar

Article

Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791

Type

Empty backbone

Use

Tags

FokI nuclease (KKR)

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 31, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
Venus-tagged Apollo-NADP+

Plasmid

#71797

Purpose

Expresses Venus-tagged Apollo-NADP+ in mammalian cells as a reporter for cytoplasmic NADP+

Depositor

Jonathan Rocheleau

Article

Cameron et al Nat Methods. 2016 Feb 15. doi: 10.1038/n

Insert

Apollo-NADP+

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge