We narrowed to 4,083 results for: Mcc;

1. pIRGAL3C

   Plasmid

   #218275

   Purpose

   Introduction of a LowTempGAL Turbo module (GAL3C) to accelerate induction of GAL promoter induction

   Depositor

   Claudia Vickers

   Article

   Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

   Insert

   fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2>GAL3C(S509P)>tENO2-fcy1(454-778)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   GAL3 (S509P)

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLC025 - pL0_StopCodon (CT)

   Plasmid

   #203905

   Purpose

   MoClo compatible L0 plasmid containing a stop codon. To allow for proper fusion with CDS1ns L0 plasmids, this plasmid adds an additional glycine and serine to the C-terminal end of a CDS and then adds a stop codon to terminate translation.

   Depositor

   Carolyn Lee-Parsons

   Article

   Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

   Insert

   Stop codon
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUN381 - pTRV2-CHLH

   Plasmid

   #203886

   Purpose

   Viral silencing plasmid targeting the CHLH gene from C. roseus. For use as a positive control for VIGS experiments.

   Depositor

   Carolyn Lee-Parsons

   Article

   Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

   Insert

   CHLH fragment
   

   Use

   RNAi

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUN1243 - pL0_pT3O (pro + 5U)

   Plasmid

   #203891

   Purpose

   T3O promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

   Depositor

   Carolyn Lee-Parsons

   Article

   Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

   Insert

   T3O promoter and 5'UTR
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUN1198 - pL0_pD4H (pro + 5U)

   Plasmid

   #203894

   Purpose

   D4H promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

   Depositor

   Carolyn Lee-Parsons

   Article

   Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

   Insert

   D4H promoter and 5'UTR
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-ALDH1A3gRNA3

   Plasmid

   #189748

   Purpose

   The construct was used for expression of gRNA for knocking out of the ALDH1A3 gene in Cas9 expressing cells.

   Depositor

   Robert Sobol

   Article

   Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

   Insert

   ALDH1A3 gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVX-anti-MIR328-3p

   Plasmid

   #153319

   Purpose

   Expresses zsGreen fused to a miR-328-3p sponge and mCherry in mammalian cells.

   Depositor

   Robert Judson-Torres

   Article

   McNeal et al Elife. 2021 Nov 23;10. pii: 70385. doi:

   Insert

   zsGreen with MIR328-3p sponge
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-anti-MIR211-5p

   Plasmid

   #153318

   Purpose

   Expresses zsGreen fused to a miR-211-5p sponge and mCherry in mammalian cells.

   Depositor

   Robert Judson-Torres

   Article

   McNeal et al Elife. 2021 Nov 23;10. pii: 70385. doi:

   Insert

   zsGreen with MIR211-5p sponge
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-Hygro-EF1A-RG-Linker-eGFP

   Plasmid

   #176071

   Purpose

   EGFP fused to the C-terminus of a RG domain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   RG
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Feb. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLentiCRISPRv2-POLB-KO-g1

    Plasmid

    #176090

    Purpose

    Cas9 plus POLB gRNA #1; contains a puromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    POLB sgRNA #1 (POLB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSMT3-FBF2 SS/R

    Plasmid

    #166976

    Purpose

    Expresses FBF-2 with SS/R mutations in repeat 5 in E. coli for purification and crystallization studies

    Depositor

    Traci Hall

    Article

    Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1

    Insert

    FBF-2 (fbf-2 Nematode)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    FBF-2 PUM RNA binding domain; amino acids 164-575…

    Promoter

    T7

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSMT3-FBF2 AQ/Y

    Plasmid

    #166975

    Purpose

    Expresses FBF-2 with AQ/Y mutations in repeat 5 in E. coli for purification and crystallization studies

    Depositor

    Traci Hall

    Article

    Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1

    Insert

    FBF-2 (fbf-2 Nematode)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    FBF-2 PUM RNA binding domain; amino acids 164-575…

    Promoter

    T7

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPQ134-STU-As

    Plasmid

    #138103

    Purpose

    Golden Gate entry vector for 4th crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymes

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQ132-STU-As

    Plasmid

    #138097

    Purpose

    Golden Gate entry vector for 2nd crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymes

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQ133-STU-As

    Plasmid

    #138100

    Purpose

    Golden Gate entry vector for 3rd crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymes

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQE30-VRN1

    Plasmid

    #159531

    Purpose

    Expresses Arabidopsis thaliana VERNALIZATION1 in E. coli

    Depositor

    Joshua Mylne

    Article

    King et al J Biol Chem. 2013 Feb 1;288(5):3198-207.

    Insert

    VERNALIZATION1 (VRN1 Mustard Weed)
    

    Use

    Tags

    Six-Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMAL-c2X-ORF24-NTD-3L_A

    Plasmid

    #138466

    Purpose

    Expresses MBP-tagged ORF24-NTD 3L_A in bacteria

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    ORF24 (ORF24 KSHV (HHV-8))
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Amino acids 1-201; amino acids L73-75 mutated to …

    Promoter

    tac

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQLink-GST-2xCTD

    Plasmid

    #138470

    Purpose

    Expresses GST-tagged 2xCTD heptapeptide repeat in bacteria

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    Rpb1 (POLR2A Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    2x CTD repeat

    Promoter

    T5

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA4/TO-ORF24 L73A_L75A-3xFLAG

    Plasmid

    #138429

    Purpose

    Expresses full-length ORF24 L73A/L75A with a C-terminal 3xFLAG tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    ORF24 (ORF24 KSHV (HHV-8))
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Amino acids L73, L75 mutated to alanine

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA4/TO-ORF24 L74A_L75A-3xFLAG

    Plasmid

    #138430

    Purpose

    Expresses full-length ORF24 L74A/L75A with a C-terminal 3xFLAG tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    ORF24 (ORF24 KSHV (HHV-8))
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Amino acids L74, L75 mutated to alanine

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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