We narrowed to 3,852 results for: SPL;

1. pEF_dCas9

   Plasmid

   #68416

   Purpose

   Transient expression of Sp-dCas9 in mammalian cells, under an EF1-alpha promoter.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D10A/H841A

   Promoter

   human EF1[alpha]

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUBC_V5_MS2-mCherry

   Plasmid

   #68420

   Purpose

   Transient expression of humanized MS2 (V75E/A81G), fused to mCherry in mammalian cells, under a UBC promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   MS2
   

   Use

   Synthetic Biology

   Tags

   V5 and mCherry

   Expression

   Mammalian

   Mutation

   MS2: V75E/A81G

   Promoter

   human UbC

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-hNox5

   Plasmid

   #69354

   Purpose

   expresses human Nox5 in mammalian cells

   Depositor

   Karl-Heinz Krause

   Article

   Banfi et al J Biol Chem. 2001 Oct 5;276(40):37594-60

   Insert

   hNox5 (NOX5 Human)
   

   Use

   Tags

   no

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-MP2

   Plasmid

   #36097

   Purpose

   3rd generation lentiviral plasmid

   Depositor

   Pantelis Tsoulfas

   Article

   Enomoto et al Exp Neurol. 2013 Oct;248:170-82. doi: 10

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP_sfCherry2(11)_H2B

   Plasmid

   #82605

   Purpose

   Expresses sfCherry2(11) tagged H2B in mammalian cells

   Depositor

   Bo Huang

   Article

   Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

   Insert

   sfCherry2(11)_H2B
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed Gly12 to Ala in sfCherry11+

   Promoter

   Available since

   June 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP_TOMM20_sfCherry2(11)

   Plasmid

   #83033

   Purpose

   Expresses sfCherry2(11) tagged TOMM20 (human) in mammalian cells cells

   Depositor

   Bo Huang

   Article

   Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

   Insert

   TOMM20_sfCherry+11
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-Tat101(AD8)-Flag

   Plasmid

   #115777

   Purpose

   pCMV-Tat101AD8-Flag allows the expression of Tat101(AD8) protein with a C-terminal flag-tag.

   Depositor

   Damian Purcell

   Article

   Khoury et al J Vis Exp. 2019 Jan 22;(143). doi: 10.37

   Insert

   HIV-1 Tat protein (AD8)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. PX856

   Plasmid

   #62888

   Purpose

   C-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64)

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Insert

   SpCas9 (aa536-1368)
   

   Use

   CRISPR

   Tags

   FKBP12, NLS, NLS SV40, and VP64

   Expression

   Mammalian

   Mutation

   Asparagine 863 to Alanine (N863A)

   Promoter

   CBh

   Available since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. PX855

   Plasmid

   #62887

   Purpose

   N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Insert

   SpCas9 (aa 2-535)
   

   Use

   CRISPR

   Tags

   FRB and NES PTK2

   Expression

   Mammalian

   Mutation

   Aspartic acide 10 to Alanine (D10A)

   Promoter

   CBh

   Available since

   March 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-SC35-cMyc

    Plasmid

    #44721

    Depositor

    Kathleen Scotto

    Article

    Shi et al Mol Cell Biol. 2008 Jan;28(2):883-95. Ep

    Insert

    SC35 (SRSF2 Human)
    

    Use

    Tags

    his and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. CMVTO-GFP-ECL-3TM-tevs-KKMP V3

    Plasmid

    #179640

    Purpose

    TEVP-inducible membrane-bound GFP RELEASE construct

    Depositor

    Xiaojing Gao

    Article

    Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

    Insert

    Membrane-bound GFP RELEASE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. CMV-dCasRx-ccdB

    Plasmid

    #221003

    Purpose

    Destination vector for Gateway cloning of ORFs into the C-terminus of dCasRx. CMV promoter. Transient expression.

    Depositor

    Benjamin Blencowe, Mikko Taipale

    Article

    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBS-HxB.S

    Plasmid

    #65415

    Purpose

    contains complete cDNA for TNC small splice variant flanked by NotI-KpnI

    Depositor

    Harold Erickson

    Article

    Aukhil et al J Biol Chem. 1993 Feb 5;268(4):2542-53.

    Insert

    tenascin C small splice variant (TNC Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAC1802_pmax-RBFOX1N-dCasRx-C

    Plasmid

    #118635

    Purpose

    Transient transfection; Expresses RBFOX1N-dCasRx-C; CAGGS promoter

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    RBFOX1N-dCasRx-C
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSCV-OPA1

    Plasmid

    #26047

    Depositor

    David Chan

    Article

    Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

    Insert

    OPA1 (OPA1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLDPuro-hsnSR100N

    Plasmid

    #35172

    Depositor

    Benjamin Blencowe

    Article

    Raj et al Mol Cell. 2011 Sep 2;43(5):843-50.

    Insert

    SRRM4 (SRRM4 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. SFG.CNb30_opt.IRES.eGFP

    Plasmid

    #22493

    Depositor

    Martin Pule

    Article

    Brewin et al Blood. 2009 Sep 21. ():.

    Insert

    Codon optimised Calcineurin B mutant 30 (PPP3R1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    L124T; K125-LA-Ins

    Promoter

    Available since

    Dec. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-NL1(-)

    Plasmid

    #15260

    Depositor

    Peter Scheiffele

    Article

    Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

    Insert

    Neuroligin 1 (Nlgn1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-Flag-Tipin

    Plasmid

    #22889

    Depositor

    Aziz Sancar

    Article

    Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

    Insert

    Tipin (TIPIN Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

20. TC1541A

    Plasmid

    #164875

    Purpose

    Split GFP reporter expressing GFP(beta1-10) following C>U editing of the target site

    Depositor

    Tian Chi

    Article

    Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

    Insert

    Split GFP(beta1-10)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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