We narrowed to 13,284 results for: sequence
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Plasmid#135938PurposeExpresses NanoLuciferase targeted to the mitochondira in mammalian cells.DepositorInsertNanoLuciferase
TagsT2 mitochondrial localization sequenceExpressionMammalianPromoterEF1aAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRI007-pGEM-PgpdA-Cas12aScaffold-BsmbI-TtrpC
Plasmid#140200PurposeCloning vector for LbCas12a crRNAs ( Step 1 of the 2-step cloning alternative). Full PgpdA sequence is reconstituted when amplifying the expression cassette for cloning in a fungal vector.DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsdLbCas12a crRNA scaffoldPromoterPgpdAAvailable SinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV-Cx35-Halo-C-in
Plasmid#71511PurposeMammalian expression plasmid encoding perch Cx35 with an internal HaloTag between amino acids 285 and 286 in the C-terminus.DepositorInsertConnexin 35
TagsHaloTag (internal to sequence)ExpressionMammalianAvailable SinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-GPI-pA
Plasmid#109490PurposeMuscle specific zebrafish expression of mKate2 fused to the 5' and 3' sequences from zebrafish folate receptor 1DepositorInsertactc1b-mKate2-GPI-pA
UseZebrafish plasmidsAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
HOXD12 (murine) pRSETa
Plasmid#8554DepositorInsertHOXD12 (Hoxd12 Mouse)
TagsHIS and XPressExpressionBacterialMutationFusion sequence is: ggatccATGAvailable SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Kp-2
Plasmid#163144PurposepLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-amylase signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionYeastPromoterAOX1Available SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCru5-ACC/ACC/ACC/ACC-mEGFP-IRES-mCherry
Plasmid#49218PurposeRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseRetroviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianPromoterViral LTR (or CMV)Available SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
MTK4b_004
Plasmid#123843PurposeEncodes spacer sequence as a Type 4b part to be used in the MTK systemDepositorInsertSpacer
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmLSm4_A
Plasmid#145883PurposeInsect Expression of DmLSm4DepositorInsertDmLSm4 (LSm-4 Fly)
TagsV5-6xHisExpressionInsectMutationtwo silent mutations compared to the sequence of …Available SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ABKAR-Kras
Plasmid#84637PurposeUnimolecular, FRET-based AMPK/BRSK activity reporter; plasma membrane targetedDepositorInsertCerulean3-FHA1-AMPKsub-cpVenus[E172]-Kras
Tags6xHis, C-terminal targeting sequence from Kras, C…ExpressionMammalianPromoterCMVAvailable SinceFeb. 15, 2022AvailabilityAcademic Institutions and Nonprofits only