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Plasmid#173100PurposeExpression of recombinant fusion protein in E. coliDepositorInsertPARP14 (PARP14 Human)
UseTagsmCeruleanExpressionBacterialMutationF1208-G1388PromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delZn3
Plasmid#173936PurposeBacterial expression of PARP1 lacking Zn3 domainDepositorInsertPARP1delZn3 (PARP1 Human)
UseTags6xHisExpressionBacterialMutationDeletion of residues 214-373; V762APromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
RP1B-PP1alpha
Plasmid#51768PurposeExpresses PP1 alpha residues 7-330DepositorInsertpp1 alpha
UseTagsHis, TEV protease site, and Thio-6ExpressionBacterialMutationContains PP1 alpha residues 7-330PromoterT7-lacAvailable sinceMarch 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
TRP1MX6-ins4
Plasmid#195041PurposepFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interferenceDepositorInsertTRP1
UseYeast genomic targetingTagsS. cerevisiae DEG1 terminator, A. gossypii TEF pr…ExpressionMutationPromoterAvailable sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
RP1B-PP1gamma
Plasmid#51770PurposeExpresses PP1 gamma residues 7-323DepositorInsertpp1 gamma
UseTagsHis, TEV protease site, and Thio-6ExpressionBacterialMutationContains PP1 gamma residues 7-323PromoterT7-lacAvailable sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1deltaART
Plasmid#174804PurposeBacterial expression of an inactive PARP1 mutant lacking the ART subdomainDepositorInsertPARP1deltaART (PARP1 Human)
UseTags6xHisExpressionBacterialMutationDeletion of residues 785-1014PromoterAvailable sinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delZn1
Plasmid#173933PurposeBacterial expression of PARP1 lacking Zn1 domainDepositorInsertPARP1delZn1 (PARP1 Human)
UseTags6xHisExpressionBacterialMutationDeletion of residues 1-96; V762APromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delZn2
Plasmid#173935PurposeBacterial expression of PARP1 lacking Zn2 domainDepositorInsertPARP1delZn2 (PARP1 Human)
UseTags6xHisExpressionBacterialMutationDeletion of residues 97-213 (V762A)PromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-HD
Plasmid#174809PurposeBacterial expression of isolated PARP1 HD subdomainDepositorInsertPARP1-HD (PARP1 Human)
UseTags6xHisExpressionBacterialMutationV762APromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD-TRP120
Plasmid#71732Purposeinducible expression of TRP120 in E. coliDepositorInsertTRP120
UseTags6xHIS and ThioredoxinExpressionBacterialMutationPromoterpBADAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only