We narrowed to 4,783 results for: Drosophila

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pCDNA3_dPKA-CAT1N17-mVenus

Plasmid

#168497

Purpose

Mammalian expression of the N-terminal 17 residues of Drosophila PKA catalytic subunit 1 C-terminally tagged by monomeric Venus.

Depositor

Haining Zhong

Article

Xiong et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

Insert

N-terminal 17 residues of Drosophila PKA catalytic subunit 1 C-terminally tagged by monomeric Venus

Expression

Mammalian

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pSN539

Plasmid

#226775

Purpose

Expresses Drosophila UNC-104(wild-type) in insect cells

Depositor

Kyoko Chiba

Article

Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

Insert

Drosophila unc-104 (unc-104 Fly)

Tags

sfGFP and 2xStrepII

Expression

Insect

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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OTV-dDAT

Plasmid

#32812

Depositor

Susan Amara

Insert

dDAT (DAT Fly)

Use

Oocyte expression

Promoter

T7

Available Since

Dec. 5, 2011

Availability

Academic Institutions and Nonprofits only
pQTEV-SUHW4

Plasmid

#34807

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

suppressor of hairy wing homolog 4 (Drosophila) (ZNF280D Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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UASp-Fz4

Plasmid

#52400

Purpose

Over-expression of Drosophila Frizzled 4

Depositor

Roel Nusse

Article

McElwain et al PLoS One. 2011;6(11):e26993. doi: 10.137

Insert

Drosophila Frizzled 4 (fz4 Fly)

Use

Drosophila expression

Promoter

UAS

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pCRII-Topo Diap3 in situ probe

Plasmid

#45602

Depositor

Jeffrey Macklis

Article

Arlotta et al Neuron. 2005 Jan 20;45(2):207-21.

Insert

Diap3 in situ probe (Diaph3 Mouse)

Use

In situ

Mutation

fragment contains bp# 1589-2321 of BC028920

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
w+attB

Plasmid

#30326

Depositor

Jeff Sekelsky

Insert

phiC31 attB, hsp70::w

Use

Drosophila transgenesis

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only

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pDCC5

Plasmid

#59984

Purpose

Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassette

Depositor

Peter Duchek

Article

Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

Insert

Cas9-D10A

Use

CRISPR

Tags

HA

Expression

Insect

Mutation

D10A

Promoter

hsp70Bb

Available Since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pBDP

Plasmid

#17566

Depositor

Gerald Rubin

Article

Pfeiffer et al Tools for neuroanatomy and neurogenetics

Insert

mini w+

Expression

Insect

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only
pRB14

Plasmid

#52522

Purpose

expresses a myc-tagged version of hCas9 in Drosophila

Depositor

Klaus Foerstemann

Article

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

S. pyogenes cas9 with humanized codon bias

Tags

myc

Expression

Insect

Promoter

tubulin

Available Since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR223-DLG3

Plasmid

#23451

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

DLG3 (DLG3 Human)

Use

Gateway Cloning

Mutation

G381A,G629T

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pActP-dtrpA1

Plasmid

#64713

Purpose

inducing calcium influx in cultured Drosophila S2 cells

Depositor

Liqun Luo

Article

Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

Insert

dtrpA1 (TrpA1 )

Expression

Insect

Available Since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

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pActP-GCaMP6m

Plasmid

#64714

Purpose

visualizing calcium in cultured Drosophila S2 cells

Depositor

Liqun Luo

Article

Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

Insert

GCaMP6m

Expression

Insect

Available Since

May 22, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-GGAC-ATGC

Plasmid

#60949

Purpose

Drosophila, backbone for donor plasmid assembly. CRISPR knock-in.

Depositor

Frank Schnorrer

Article

Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pAc-y1sgRNA-Cas9

Plasmid

#49331

Purpose

Expresses sgRNA targeting yellow gene and Cas9-Puro in Drosophila S2 cells

Depositor

Ji-Long Liu

Article

Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

Inserts

Cas9
dU6-sgRNA
y1 sgRNA (y Fly)

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Insect

Mutation

Human codon optimised

Promoter

Actin-5c and Drosophila U6

Available Since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only

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PRExpress

Plasmid

#122486

Purpose

A non-leaky heat-inducible gene expression system for Drosophila. (GenBank: MF058598.1)

Depositor

Renato Paro

Article

Akmammedov et al Sci Rep. 2017 Jul 31;7(1):6899. doi: 10.

Inserts

LacZ
poly-PRE

Expression

Insect

Available Since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-Cdh1wt (570)

Plasmid

#39877

Depositor

Jonathon Pines

Insert

FZR1 (FZR1 Human)

Tags

GST

Expression

Bacterial

Available Since

March 5, 2013

Availability

Academic Institutions and Nonprofits only
act-AsCpf1

Plasmid

#73917

Purpose

expression plasmid of AsCpf1

Depositor

Simon Bullock

Article

Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

Insert

hAsCpf1

Tags

NLS-3xHA

Expression

Insect

Promoter

act5C

Available Since

March 8, 2016

Availability

Academic Institutions and Nonprofits only

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pCFD3-ebony

Plasmid

#83380

Purpose

Drosophila gRNA expression plasmid targets ebony

Depositor

Richard Padgett

Article

Kane et al G3 (Bethesda). 2017 Jan 5;7(1):87-93. do

Insert

ebony (e Fly)

Use

CRISPR

Expression

Insect

Available Since

Oct. 28, 2016

Availability

Academic Institutions and Nonprofits only

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p3xP3-EGFP.vas-int.NLS

Plasmid

#60948

Purpose

Drosophila, p3xP3-EGFP.vas-int.NLS.attB was digested SpeI to get rid of the attB site. used for RMCE. CRISPR.

Depositor

Frank Schnorrer

Article

Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

Insert

vas-phiC31 integrase.NLS

Use

Rmce

Promoter

vasa

Available Since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only