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We narrowed to 13,699 results for: TIM

Showing: 3161 - 3200 of 13699 results

  2. pCMV-T7-ABE8e-nSpCas9-VRQR-P2A-EGFP (KAC1309)

    Plasmid
    #185913
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized ABE8e-nSpCas9-VRQR-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185913-plasmid-map-sequence-id-367365

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  6. pET24a-ncOGT-FL

    Plasmid
    #190821
    Purpose
    For expression of full-length human ncOGT in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.
    Depositor
    Gerald Hart
    Article
    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.
    Insert
    O-GlcNAc Transferase (OGT Human)
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Codon optimized for expression in E.coli
    Promoter
    T7
    Available since
    Jan. 9, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    190821-plasmid-map-sequence-id-379184

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  7. pL

    Plasmid
    #196286
    Purpose
    35S promoter-driven expression of the positive-sense TSWV L RNA segment encoding codon-optimized L (RdRp)
    Depositor
    Zhenghe Li
    Article
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV L antigenome encoding codon-optimized L (RdRp)
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196286-plasmid-map-sequence-id-390014

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  11. pBAD-tdTEVDB_C-15A/G4C

    Plasmid
    #177205
    Purpose
    Permuted td intron with evolved initiation sequence for the expression of circular mRNA
    Depositor
    Stephen Fried
    Article
    Lee et al ACS Cent Sci. 2021 Oct 27;7(10):1736-175
    Insert
    Permuted GFP with evolved initiation sequence for circular mRNA
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Permuted sfGFP after residue 52 & Changed Cyt…
    Promoter
    araBAD
    Available since
    Sept. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177205-plasmid-map-sequence-id-373215

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  18. FRB-CD28TMD-CTEVp(WT)-CS(M)-tTA

    Plasmid
    #137827
    Purpose
    MESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal WT TEV protease, M cleavage sequence, and tTA transcription factor
    Depositor
    Joshua Leonard
    Article
    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d
    Insert
    FRB-CD28TMD-CTEVp(WT)-CS(M)-tTA
    Use
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    137827-plasmid-map-sequence-id-311923

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  19. pRDA_816

    Plasmid
    #201157
    Purpose
    EnAsCas12a with a deactivated DNAse domain (D908A), N' 5x tandem ALFA-tags and C' VP64 and NLS for CRISPRa
    Depositor
    John Doench
    Article
    Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:
    Insert
    5x-ALFA tag-dEnAsCas12a-VP64
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    D908A
    Promoter
    EFS
    Available since
    June 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    201157-plasmid-map-sequence-id-396590

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  21. pUC57-mitoTPNOX

    Plasmid
    #87854
    Purpose
    This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.
    Depositor
    Vamsi Mootha
    Article
    Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/
    Insert
    mitoTPNOX
    Use
    Tags
    C-terminal FLAG tag with a linker and mitochondri…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    87854-plasmid-map-sequence-id-175127

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  22. pKM7

    Plasmid
    #154164
    Purpose
    mCherry expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.
    Depositor
    Kevin Maringer
    Article
    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0
    Insert
    mCherry
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    IE1 promoter and hr5 enhancer from Autographa cal…
    Available since
    Aug. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    154164-plasmid-map-sequence-id-309401

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  30. pET28a-HsSENP2

    Plasmid
    #71466
    Purpose
    Encodes human full length SENP2 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).
    Depositor
    Jorge Eduardo Azevedo
    Article
    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:
    Insert
    SUMO1/sentrin specific peptidase 2 (SENP2 Human)
    Use
    Tags
    Hexahistidine
    Expression
    Bacterial
    Mutation
    Gene optimised for E. coli expression (Genscript)
    Promoter
    T7
    Available since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  35. pVDL9.3

    Plasmid
    #168299
    Purpose
    Secretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyA
    Depositor
    Víctor de Lorenzo
    Article
    Tzschaschel et al Nat Biotechnol. 1996 Jun;14(6):765-9. do
    Insert
    Segment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    LacI-Plac
    Available since
    June 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    168299-plasmid-map-sequence-id-335286

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  37. pET28a-HsSENP1

    Plasmid
    #71465
    Purpose
    Encodes human full length SENP1 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).
    Depositor
    Jorge Eduardo Azevedo
    Article
    Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:
    Insert
    SUMO1/sentrin specific peptidase 1 (SENP1 Human)
    Use
    Tags
    Hexahistidine
    Expression
    Bacterial
    Mutation
    Gene optimised for E. coli expression (Genscript)
    Promoter
    T7
    Available since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  39. pCas9D10A_GFP

    Plasmid
    #44720
    Purpose
    Co-expression of human codon-optimized Cas9 (D10A) mutant nickase and GFP, plasmid optimized for expression in human pluripotent stem cells
    Depositor
    Kiran Musunuru
    Article
    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.
    Insert
    Cas9D10A-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP
    Expression
    Mammalian
    Mutation
    D10A mutation- nickase mutant
    Promoter
    CAG
    Available since
    May 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    44720-plasmid-map-sequence-id-179979

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Showing: 3161 - 3200 of 13699 results