Addgene

We narrowed to 40,542 results for: tro

Showing: 3161 - 3200 of 40542 results

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pEPYC1CB0325

Plasmid

#154670

Purpose

MoClo Level 1 luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

35s(ΔRWPRK2) Firefly luciferase expression cassette [35s(ΔRWPRK2)::TMV::LucF::Flag::ocsT]

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0189

Plasmid

#154653

Purpose

MoClo Level 1 luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_058 Firefly luciferase expression cassette [MinSyn_058::TMV::LucF::Flag::ocsT]

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0321

Plasmid

#154666

Purpose

MoClo Level 1 luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

35s(ΔHSF+) Firefly luciferase expression cassette [35s(ΔHSF+)::TMV::LucF::Flag::ocsT]

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0453

Plasmid

#154709

Purpose

MoClo Level 1 luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_022 Firefly luciferase expression cassette [MinSyn_022::TMV::LucF::Flag::ocsT]

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0473

Plasmid

#154722

Purpose

MoClo Level 1 luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MinSyn_107 Firefly luciferase expression cassette [MinSyn_107::TMV::LucF::Flag::ocsT]

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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GtCCR1_EYFP_pcDNA3.1

Plasmid

#159120

Purpose

This plasmid encodes GtCCR1 rhodopsin domain

Depositor

John Spudich

Article

Sineshchekov et al Proc Natl Acad Sci U S A. 2017 Nov 7;114

Insert

GtCCR1

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMX234

Plasmid

#23006

Depositor

Linda Wordeman

Article

Stumpff et al Dev Cell. 2008 Feb . 14(2):252-62.

Insert

mRFP-CenpB

Use

Tags

mRFP1.0

Expression

Mammalian

Mutation

deleted CenpB amino acids 169-606, T145S

Promoter

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-SYNZIP7

Plasmid

#80663

Purpose

Encodes the synthetic coiled coil peptide SYNZIP7

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP7

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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Pu.1 NGFR

Plasmid

#80132

Purpose

overexpression of Pu.1

Depositor

Ellen Rothenberg

Article

Del Real et al Development. 2013 Mar;140(6):1207-19. do

Insert

Pu.1 (Spi1 Mouse)

Use

Retroviral

Tags

IRES-Ngfr

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pICSL80012

Plasmid

#50331

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, YFP variant of GFP (A. victoria)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

March 7, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL00002

Plasmid

#50347

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

Linker for joining modules with 3' TTCG overhangs to modules with 5' GTGG overhangs

Use

Plant expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH71292

Plasmid

#50280

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter and 5'UTR, RbcS2 (S. lycopersicum)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH71311

Plasmid

#50282

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter and 5'UTR, RbcS3A (S. lycopersicum)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL00001

Plasmid

#50346

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

Linker for joining modules with 3' GTGG overhangs to modules with 5' TTCG overhangs

Use

Plant expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL50012

Plasmid

#50311

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

C terminal V5 tag (Simian Virus 5)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL50013

Plasmid

#50312

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

C terminal T7 tag (bacteriophage T7 gene 10)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH71301

Plasmid

#50281

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter and 5'UTR, RbcS2 (S. lycopersicum)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH37431

Plasmid

#50306

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

signal peptide, RAmy3A (O. sativa)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH42760

Plasmid

#50257

Depositor

Nicola Patron, Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter, spm (Z. mays)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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RCAS (A) Flu cTbx4 (CT#700)

Plasmid

#13925

Depositor

Cliff Tabin

Article

Logan et al Development. 1998 Aug . 125(15):2825-35.

Insert

Tbx4 (TBX4 Chicken)

Use

Retroviral; Avian expression

Tags

Flu (3xHA)

Expression

Mutation

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pENTR-SYNZIP8

Plasmid

#80664

Purpose

Encodes the synthetic coiled coil peptide SYNZIP8

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP8

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-SYNZIP22

Plasmid

#80676

Purpose

Encodes the synthetic coiled coil peptide SYNZIP22

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP22

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pMDLg/pRRE

Plasmid

#12251

Purpose

3rd generation lentiviral packaging plasmid; Contains Gag and Pol; also requires pRSV-Rev (Addgene#12253) and envelope expressing plasmid (Addgene#12259)

Depositor

Didier Trono

Article

Dull et al J Virol. 1998 Nov . 72(11):8463-71.

Insert

HIV-1 GAG/POL

Use

Lentiviral; Packaging

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pNFkB:eGFP

Plasmid

#44922

Depositor

John Rawls

Article

Kanther et al Gastroenterology. 2011 Jul;141(1):197-20

Insert

EGFP

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pGL0_24 [pBBR1-UP ORI]

Plasmid

#198937

Purpose

Level 0 part

Depositor

Charlie Gilbert, Cultivarium Tools, Nili Ostrov

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pBBR1-UP ORI

Use

Synthetic Biology

Tags

Expression

Mutation

S100L (C299T)

Promoter

Available since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSK9065

Plasmid

#84345

Purpose

tightly controlled expression of mRFP fusion proteins in S. aureus

Depositor

Neville Firth

Article

Brzoska et al Appl Environ Microbiol. 2013 May;79(9):3

Type

Empty backbone

Use

Tags

mRFPmars

Expression

Bacterial

Mutation

Promoter

Pxyl/tet

Available since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV PRDM16

Plasmid

#15504

Depositor

Bruce Spiegelman

Article

Seale et al Cell Metab. 2007 Jul . 6(1):38-54.

Insert

PRDM16 (isoform 4) (Prdm16 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only

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pAct-dCBEevoCDA1

Plasmid

#195515

Purpose

Ubiquitous expression of dCBEevoCDA1 in Drosophila

Depositor

Michael Boutros

Article

Doll et al Sci Adv. 2023 Sep;9(35):eadj1568. doi: 1

Insert

dCBE-evoCDA1

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

act5C

Available since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pEZYmyc-His

Plasmid

#18701

Purpose

pcDNA3.1(-)/Myc-His B converted Gateway Destination vector for expressing C-terminal myc-His tagged fusion proteins in mammalian cells

Depositor

Yu-Zhu Zhang

Article

Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

Type

Empty backbone

Use

Tags

Myc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 26, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_nMagHigh1-EGFP-CAAX

Plasmid

#67300

Purpose

Blue light dependent photoswitching system

Depositor

Moritoshi Sato

Article

Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

Insert

nMagHigh1-EGFP-CAAX

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL1_37

Plasmid

#199044

Purpose

Level 1 plasmid, barcoded ORI

Depositor

Charlie Gilbert, Cultivarium Tools, Nili Ostrov

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

RSF1010 ORI plus NGS barcode 14

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL1_4

Plasmid

#199036

Purpose

Level 1 plasmid, barcoded ORI

Depositor

Charlie Gilbert, Cultivarium Tools, Nili Ostrov

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pIP501 ORI plus NGS barcode 4

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL0_9 [pUB110 ORI]

Plasmid

#198924

Purpose

Level 0 part

Depositor

Charlie Gilbert, Cultivarium Tools, Nili Ostrov

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pUB110 ORI

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Ac5-STABLE2-neo

Plasmid

#32426

Depositor

James Sutherland, Rosa Barrio

Article

Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

Insert

Flag-mCherry-T2A-GFP-T2A-neo

Use

Tags

Expression

Insect

Mutation

Promoter

Actin5C

Available since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pAL120-TK

Plasmid

#22053

Depositor

Maria Castro

Article

King et al J Virol. 2008 May . 82(9):4680-4.

Insert

HSV Type 1 thymidine kinase

Use

Adenoviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4-TO-Hygromycin-sfGFP-MAP

Plasmid

#44100

Depositor

Dannel McCollum

Article

Ma et al Mol Cell Proteomics. 2012 Aug;11(8):501-

Type

Empty backbone

Use

Tags

sfGFP-MAP

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pME-GAL4-ERT2-VP16

Plasmid

#82588

Purpose

Middle entry vector containing GAL4 fused to ERT2 and the VP16 transcriptional activation domain for tamoxifen-inducible UAS-promoted transgene expression

Depositor

Kryn Stankunas

Article

Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

Insert

GAL4-ERT2-VP16

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL0_12 [pAMβ1 ORI]

Plasmid

#198926

Purpose

Level 0 part

Depositor

Charlie Gilbert, Cultivarium Tools, Nili Ostrov

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pAMβ1 ORI

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
myc-NLRC5

Plasmid

#37509

Depositor

Thomas Kufer

Article

Neerincx et al J Immunol. 2012 May 15;188(10):4940-50.

Insert

NLRC5 (NLRC5 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 9, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pIVT2

Plasmid

#122140

Purpose

For making cRNA appropriate for microinjection

Depositor

Carmen Williams

Article

Winuthayanon et al Elife. 2015 Dec 1;4:e10453. doi: 10.7554

Type

Empty backbone

Use

For in vitro transcription

Tags

Expression

Mutation

Promoter

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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