We narrowed to 8,589 results for: Set

1. AAVS1_Puro_hPGK1_EBFP_Donor

   Plasmid

   #178089

   Purpose

   HDR donor plasmid to introduce the EBFP (T65S/F145Y) cassette to AAVS1 using AAVS1 T2 sgRNA.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   EBFP (T65S/F145Y)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Human PGK1

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-KO-PGK-Blast

   Plasmid

   #227265

   Purpose

   Empty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a PGK-Blast cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK1035

   Plasmid

   #8832

   Depositor

   David Waugh

   Article

   Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

   Insert

   TVMV protease
   

   Tags

   His and MBP (cleaved)

   Expression

   Bacterial

   Mutation

   synthetic gene with codons optimized for E. coli

   Available Since

   Feb. 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT-GEM(2)

   Plasmid

   #62894

   Purpose

   Trojan-Gal4 Expression Module in Phase 2. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Type

   Empty backbone

   Available Since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT-GEM(1)

   Plasmid

   #62893

   Purpose

   Trojan-Gal4 Expression Module in Phase 1. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Type

   Empty backbone

   Available Since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL4.10/RSV_XS3B-miniXon_Luciferase

   Plasmid

   #217302

   Purpose

   Plasmid containing the SF3B3-XS3B/Firefly luciferase cassette, a modification of the SF3B3-miniXon system. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-XS3B by L

   Depositor

   Beverly L. Davidson

   Insert

   XS3B-miniXon
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   Kozak ATG initiation sequence in middle exon

   Promoter

   RSV

   Available Since

   June 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKI-cjACTB-IRES-ERT2CreERT2-IRES-Hyg

   Plasmid

   #159927

   Purpose

   Gene targeting

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Stem Cell Res. 2021 Jan 8;51:102164. doi

   Insert

   ERT2CreERT2
   

   Use

   Marmoset targeting

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFvL118

   Plasmid

   #64752

   Purpose

   Recombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 epitope tag, Hygro selection

   Depositor

   Fred van Leeuwen

   Article

   Verzijlbergen et al Proc Natl Acad Sci U S A. 2010 Jan 5;107

   Insert

   RITE cassette
   

   Use

   Rite cassette

   Promoter

   none

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTW081

   Plasmid

   #64755

   Purpose

   Recombination-Induced Tag Exchange (RITE) Cassette, T7 to an HA epitope tag, Hygro selection

   Depositor

   Fred van Leeuwen

   Article

   Verzijlbergen et al Proc Natl Acad Sci U S A. 2010 Jan 5;107

   Insert

   RITE cassette
   

   Use

   Rite cassette

   Promoter

   none

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHPK1212

    Plasmid

    #8834

    Depositor

    David Waugh

    Article

    Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

    Insert

    tobacco vein mottling virus protease, catalytic domain
    

    Tags

    MBP

    Expression

    Bacterial

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti_dNmeCas9

    Plasmid

    #120077

    Purpose

    dNmeCas9 with no sgRNA cassette lenti construct

    Depositor

    Erik Sontheimer

    Article

    Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

    Insert

    dNmeCas9
    

    Use

    Lentiviral

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. 3087 pBS Bcl-2-Ig

    Plasmid

    #8809

    Depositor

    Stanley Korsmeyer

    Article

    Seto et al EMBO J 1988 Jan;7(1):123-31.

    Insert

    Bcl-2-Ig (BCL2 Human)
    

    Expression

    Bacterial

    Available Since

    Nov. 14, 2005

    Availability

    Academic Institutions and Nonprofits only

13. C-term AAV-SpCas9-VRQR-gRNA_entry (HES1477)

    Plasmid

    #242660

    Purpose

    CBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and pU6 SpCas9 gRNA entry cassette

    Depositor

    Benjamin Kleinstiver

    Insert

    pAAV-pCbh-BPNLS-NpuC-SpCas9-VRQR-BPNLS-WPRE-bGH_PA-SpCas9_gRNA_entrycassette
    

    Use

    AAV and CRISPR

    Tags

    BPNLS

    Mutation

    VRQR mutations in SpCas9(S55R/D1135V/G1218R/R1335…

    Promoter

    CBh

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. S111

    Plasmid

    #174549

    Purpose

    Constitutive PhCMV-driven mammalian expression vector of CDH-1-TF cassette (PhCMV-Gal4-Bcl-XL-P2A-LD3-Rel65-pA)

    Depositor

    Bruno Correia

    Article

    Shui et al Nat Commun. 2021 Oct 1;12(1):5754. doi:

    Insert

    BclxL and designed protein LD3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. S112

    Plasmid

    #174550

    Purpose

    Constitutive PhCMV-driven mammalian expression vector of CDH-2-TF cassette (PhCMV-Gal4-Bcl2-P2A-LD3-Rel65-pA)

    Depositor

    Bruno Correia

    Article

    Shui et al Nat Commun. 2021 Oct 1;12(1):5754. doi:

    Insert

    Bcl2 and designed protein LD3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTW087

    Plasmid

    #64756

    Purpose

    Recombination-Induced Tag Exchange (RITE) Cassette, HA-6xHIS to a T7 epitope tag, Hygro selection

    Depositor

    Fred van Leeuwen

    Article

    De Vos et al EMBO Rep. 2011 Sep 1;12(9):956-62. doi:

    Insert

    RITE cassette
    

    Use

    Rite cassette

    Promoter

    none

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28 His6-mEGFP-D4

    Plasmid

    #226372

    Purpose

    Production of recombinant mEGFP-D4, a fluorescent probe binding cholesterol

    Depositor

    Catherine Tomasetto

    Article

    Eichler et al Methods Mol Biol. 2025;2888:101-118. doi

    Insert

    D4 (pfoA Clostridium perfringens)
    

    Tags

    His6 and monomeric EGFP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOSIP-KH-RBS2-dCas9

    Plasmid

    #182740

    Purpose

    Integrative plasmid at the HK022 site in E. coli carrying dCas9 under the control of a Ptet promoter

    Depositor

    David Bikard

    Article

    Rousset et al PLoS Genet. 2018 Nov 7;14(11):e1007749.

    Insert

    dCas9
    

    Use

    CRISPR; Integrative vector

    Promoter

    Ptet

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDOC-GG

    Plasmid

    #149377

    Purpose

    Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring

    Depositor

    Mark Pallen

    Article

    Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi

    Inserts

    Fragment containing a kanamycin resistance cassette
    Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
    Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
    Fragment containing an I-SceI recognition sequence
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX-evoCas9

    Plasmid

    #107550

    Purpose

    Expresses the M495V/Y515N/K526E/R661Q (evoCas9) SpCas9 variant in mammalian cells

    Depositor

    Anna Cereseto

    Article

    Casini et al Nat Biotechnol. 2018 Jan 29. pii: nbt.40

    Insert

    Humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    M495V/Y515N/K526E/R661Q

    Promoter

    CBh

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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