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pPOTv7-hygro-mScarletI

Plasmid

#179820

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScarlet-I

Use

Unspecified

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-g418-mNG

Plasmid

#179821

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNG

Use

Unspecified

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-g418-mScarletI

Plasmid

#179822

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScarlet-I

Use

Unspecified

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-g418-mScarlet

Plasmid

#179823

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScarlet

Use

Unspecified

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-10Ty

Plasmid

#179807

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

10xTy

Use

Unspecified

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-hygro-10Ty

Plasmid

#179808

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

10xTy

Use

Unspecified

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-SNAPtag

Plasmid

#179786

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

SNAP

Use

Unspecified

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pre_IV_CS6_4323_v1

Plasmid

#126859

Purpose

4323 b scaffold from compatibility group 4, made by method IV, insert based on de Bruijn sequence with the an order of 7

Depositor

Hendrik Dietz

Publication

Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

Insert

IV_CS6_4323_v1

Use

Synthetic Biology

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pre_IV_CS10_1033_v1

Plasmid

#126860

Purpose

4x 1033 b long scaffolds divided by Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 10, made by method IV, yields linear 1033 b long scaffolds after Zn incubation

Depositor

Hendrik Dietz

Publication

Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

Insert

IV_CS10_1033_v1

Use

Synthetic Biology

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-3Ty

Plasmid

#221047

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

3xTy epitopes

Use

Unspecified

Promoter

read through

Available since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pPOTv7-g418-10FLAG

Plasmid

#179812

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

10x FLAG

Use

Unspecified

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSB1A3_GFP_dT25

Plasmid

#89664

Purpose

expression of doubleTAL staple protein dT25 with N-terminally fused cycle3-GFP

Depositor

Hendrik Dietz

Publication

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

GFP_dT25

Tags

His6

Expression

Bacterial

Promoter

T7

Available since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pSB1A3_GFP_dT19

Plasmid

#89661

Purpose

expression of doubleTAL staple protein dT19 with N-terminally fused cycle3-GFP

Depositor

Hendrik Dietz

Publication

Praetorius et al Science. 2017 Mar 24;355(6331). pii: eaa

Insert

GFP_dT19

Tags

His6

Expression

Bacterial

Promoter

T7

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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ACVA

Plasmid

#29421

Depositor

Steven Vogel

Publication

Koushik et al PLoS One. 2009 Nov 25;4(11):e8031. doi:

Insert

Amber-Cerulean-Venus-Amber

Use

Stoichiometry standard

Expression

Mammalian

Available since

June 23, 2011

Availability

Academic Institutions and Nonprofits only
VCAA

Plasmid

#29422

Depositor

Steven Vogel

Publication

Koushik et al PLoS One. 2009 Nov 25;4(11):e8031. doi:

Insert

Venus-5-Cerulean-5-Amber-6-Amber

Use

Stoichiometry standard

Expression

Mammalian

Available since

June 23, 2011

Availability

Academic Institutions and Nonprofits only
ACAV

Plasmid

#29420

Depositor

Steven Vogel

Publication

Koushik et al PLoS One. 2009 Nov 25;4(11):e8031. doi:

Insert

Amber-Cerulean-Amber-Venus

Use

Stoichiometry standard

Expression

Mammalian

Available since

June 23, 2011

Availability

Academic Institutions and Nonprofits only
pLEW100v5-NEO-T7-sgRNA

Plasmid

#245118

Purpose

Plasmid backbone to for inserting a T7 promoter driven guide RNA into the rDNA spacer of T. brucei

Depositor

Monica Mugnier

Publication

Smith et al Nature. 2026 Apr 8. doi: 10.1038/s41586-

Type

Empty backbone

Use

CRISPR

Available since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pPOTv6-blast-3Ty:mScarletI:3Ty

Plasmid

#201090

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScaret-I

Use

Unspecified

Promoter

read through

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-BFP

Plasmid

#190258

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Sue Vaughan

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

BFP

Use

For endogenous gene tagging

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-hygro-BFP

Plasmid

#190260

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Sue Vaughan

Publication

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

BFP

Use

For endogenous gene tagging

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge