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pKMW124_dCas9

Plasmid

#244821

Purpose

Mammalian expression of catalytically inactive Cas9 (dCas9)

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

dCas9 (WT)

Use

CRISPR

Tags

3xFLAG-SV40 NLS and Nucleoplasmin NLS

Expression

Mammalian

Mutation

D10A/H840A

Promoter

CAG, U6

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pHS26_10xHis_MBP_TEV_VRER-Cas9

Plasmid

#244829

Purpose

Bacterial expression of VRER Cas9 for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

VRER Cas9

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Mutation

D1135V/G1218R/R1335E/T1337R

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHS16_10xHis_MBP_TEV_xPBA-Cas9

Plasmid

#244830

Purpose

Bacterial expression of xPBA Cas9 for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

xPBA Cas9

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHS25_10xHis_MBP_TEV_EQR-Cas9

Plasmid

#244828

Purpose

Bacterial expression of EQR Cas9 for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

EQR Cas9

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Mutation

D1135E/R1335Q/T1337R

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pVSr-GhOMT1

Plasmid

#234932

Purpose

This virus-induced gene silencing (VIGS) reporter vector is used to evaluate the silencing of GhOMT1 gene

Depositor

Guoli Song

Article

Hao et al Plant Commun. 2025 May 19:101379. doi: 1

Insert

Partial coding region sequence of GhOMT1

Expression

Plant

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-48xHSPA1B array

Plasmid

#236378

Purpose

Expression of array of 48 crRNAs targeting HSPA1B

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

48xHSPA1B crRNA array (HSPA1B Human)

Use

CRISPR and Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-48xNFIL3 array

Plasmid

#236379

Purpose

Expression of array of 48 crRNAs targeting NFIL3

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

48xNFIL3 crRNA array (NFIL3 Human)

Use

CRISPR and Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-48xS100A10 array

Plasmid

#236381

Purpose

Expression of array of 48 crRNAs targeting S100A10

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

48xS100A10 crRNA array (S100A10 Human)

Use

CRISPR and Lentiviral

Available Since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-HyperdFnCas12a-EGFP-NLS-3xFlag

Plasmid

#236376

Purpose

Overexpression of HyperdFnCas12a in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

HyperdFnCas12a

Use

CRISPR and Lentiviral

Mutation

E184R, T279R, D340R, D409R

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dFnCas12a-EGFP-NLS-3xFlag

Plasmid

#236375

Purpose

Overexpression of dFnCas12a in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

Insert

dFnCas12a

Use

CRISPR and Lentiviral

Mutation

WT

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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