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VV073: 1xCox8 - GCaMP5G in fubi

Plasmid

#58509

Purpose

Expresses the calcium sensor GCaMP5G in the mitochondria

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

1xCox8 - GCaMP5G

Use

Lentiviral

Expression

Mammalian

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pRAM29

Plasmid

#234315

Purpose

G264A Cat-RNA U1367, Catalytically dead ribozyme control

Depositor

James Chappell

Article

Kalvapalle et al Nat Biotechnol. 2025 Mar 18. doi: 10.103

Insert

Splicing-deficient mutant of ribozyme

Use

Synthetic Biology

Promoter

P. CymRC

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pET30-T7-lacO-His-sfGFP

Plasmid

#180754

Purpose

pET30 vector with sfGFP-S-tag inserted between the Bgl II and T7 terminator

Depositor

Michael Black

Article

Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

Insert

superfolder green fluorescent protein

Tags

strep tag

Expression

Bacterial

Mutation

G140C- Please see depositor comments

Available Since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pBEVY-U

Plasmid

#51230

Purpose

bi-directional expression vector for yeast, constitutively active, URA3 selection

Depositor

Charles Miller

Article

Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

Type

Empty backbone

Expression

Yeast

Promoter

GPD/ADH1

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pmEGFP-N1

Plasmid

#217486

Purpose

Expression of monomeric Enhanced Green Fluorescent Protein

Depositor

Bertrand Collet

Article

Chaumont et al BMC Genomics. 2024 Jun 29;25(1):650. doi

Insert

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMC-1-AtUbi10-6

Plasmid

#197700

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

AtUbi10 promoter

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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BFP dest clone

Plasmid

#71825

Purpose

Lentiviral introduction of BFP reporter (Point mutation H66 in mEGFP results in BFP expression)

Depositor

Jacob Corn

Article

Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pMC-9-35S-11

Plasmid

#197759

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

35S terminator

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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GST-Pin1

Plasmid

#19027

Depositor

Michael Yaffe

Article

Yaffe et al Science. 1997 Dec 12. 278(5345):1957-60.

Insert

Pin1 (PIN1 Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only

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pGRACE

Plasmid

#230910

Purpose

Tracing of CRISPR activity in whole animals.

Depositor

Michael Boutros

Article

Port et al bioRxiv 2024.11.26.625385

Insert

GFP

Use

CRISPR

Expression

Insect

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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