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pKG3777_pGEEC584_pShip-EFS(promoter)-CMV(UTR)-mRuby2-bGH

Plasmid

#239746

Purpose

Plasmid expressing mRuby2 from the EFS canonical promoter with the CMV 5' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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pKG3668_pGEEC578_pShip-EFS-rtTA-P2A-iRFP720-bGH

Plasmid

#239741

Purpose

Plasmid expressing a doxycycline-inducible transcriptional activator

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

rtTA-P2A-iRFP720

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG3831_pGEEC579_pShip-UbC(no_intron)-mRuby2-bGH

Plasmid

#239742

Purpose

Plasmid expressing mRuby from a UbC promoter lacking an intron, used for modRNA production

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG3774_pGEEC580_pShip-EF1a(no_intron)-mRuby2-bGH

Plasmid

#239743

Purpose

Plasmid expressing mRuby from an EF1a promoter lacking an intron, used for modRNA production

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG3775_pGEEC582_pShip-hPGK(promoter)-CMV(UTR)-mRuby2-bGH

Plasmid

#239744

Purpose

Plasmid expressing mRuby2 from the hPGK canonical promoter with the CMV 5' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG3776_pGEEC583_pShip-hPGK(promoter)-EFS(UTR)-mRuby2-bGH

Plasmid

#239745

Purpose

Plasmid expressing mRuby2 from the hPGK canonical promoter with the EFS 5' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG3778_pGEEC585_pShip-EFS(promoter)-hPGK(UTR)-mRuby2-bGH

Plasmid

#239747

Purpose

Plasmid expressing mRuby2 from the EFS canonical promoter with the hPGK 5' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG3779_pGEEC586_pShip-CMV(promoter)-EFS(UTR)-mRuby2-bGH

Plasmid

#239748

Purpose

Plasmid expressing mRuby2 from the CMV canonical promoter with the EFS 5' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG3780_pGEEC587_pShip-CMV(promoter)-hPGK(UTR)-mRuby2-bGH

Plasmid

#239749

Purpose

Plasmid expressing mRuby2 from the CMV canonical promoter with the hPGK 5' UTR

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG2436_pGEEC532_pShip-EF1a-mRuby2-P2A-PuroR-bGH

Plasmid

#239728

Purpose

Plasmid expressing mRuby2 and PuroR with the EF1a promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2-P2A-PuroR

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG2437_pGEEC533_pShip-EFS-mRuby2-P2A-PuroR-bGH

Plasmid

#239729

Purpose

Plasmid expressing mRuby2 and PuroR with the EFS promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2-P2A-PuroR

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG2438_pGEEC534_pShip-CMV-mRuby2-P2A-PuroR-bGH

Plasmid

#239730

Purpose

Plasmid expressing mRuby2 and PuroR with the CMV promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2-P2A-PuroR

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG2439_pGEEC535_pShip-hPGK-mRuby2-P2A-PuroR-bGH

Plasmid

#239731

Purpose

Plasmid expressing mRuby2 and PuroR with the hPGK promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2-P2A-PuroR

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG2441_pGEEC537_pShip-CAG-mRuby2-P2A-PuroR-bGH

Plasmid

#239733

Purpose

Plasmid expressing mRuby2 and PuroR with the CAG promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2-P2A-PuroR

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG3660_pGEEC570_pShip-ZF10.BSx8-ybTATA-spacer-mRuby2-bGH

Plasmid

#239739

Purpose

Plasmid expressing mRuby2 from a SynZiFTR promoter, Induced with pKG2309

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG2309_pGEEC524_pShip-EFS-ZF10-NS3-p65-bGH

Plasmid

#239722

Purpose

Plasmid expressing a grazoprevir-inducible transcriptional activator

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

ZF10-NS3-p65

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG1367_pPV1-hPGK

Plasmid

#239691

Purpose

BsaI Golden Gate assembly plasmid encoding the hPGK promoter

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

hPGK promoter

Use

Synthetic Biology

Mutation

Removal of BsaI cut sites

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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pKG2387_pPV2-mRuby2-P2A-PuroR

Plasmid

#239696

Purpose

BsaI Golden Gate assembly plasmid encoding the mRuby2-P2A-PuroR CDS

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

mRuby2-P2A-PuroR

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG3659_pPV2-rtTA-P2A-iRFP720

Plasmid

#239698

Purpose

BsaI Golden Gate assembly plasmid encoding the rtTA-P2A-iRFP720 CDS

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Insert

rtTA-P2A-iRFP720

Use

Synthetic Biology

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
ADEPT-pCas9

Plasmid

#238032

Purpose

Encodes Cas9 (ssrA degradation-tagged) under a tetracycline promoter (pTet) on a low-copy replication origin (SC101) as part of the ADEPT system.

Depositor

Lingchong You

Article

Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

Insert

spCas9

Use

Synthetic Biology

Tags

ssrA

Promoter

tet

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR2b-Paxillin-EGFP

Plasmid

#215529

Purpose

Gateway entry vector with EGFP-tagged paxillin

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PXN (PXN Human)

Use

Gateway entry vector

Tags

EGFP

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pENTR2b-Src(WT)

Plasmid

#215459

Purpose

Gateway entry vector with Src wild-type (WT)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Src (SRC Chicken)

Use

Gateway entry vector

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
ADEPT-pTarget-Msp4

Plasmid

#238037

Purpose

Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.

Depositor

Lingchong You

Article

Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

Insert

sfGFP

Use

Synthetic Biology

Promoter

lac

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
ADEPT-pTarget-Msp1

Plasmid

#238034

Purpose

Encodes sfGFPunder lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.

Depositor

Lingchong You

Article

Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

Insert

sfGFP

Use

Synthetic Biology

Promoter

lac

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
ADEPT-pTarget-Msp2

Plasmid

#238035

Purpose

Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.

Depositor

Lingchong You

Article

Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

Insert

sfGFP

Use

Synthetic Biology

Promoter

lac

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
ADEPT-pTarget-Msp3

Plasmid

#238036

Purpose

Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.

Depositor

Lingchong You

Article

Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

Insert

sfGFP

Use

Synthetic Biology

Promoter

lac

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
ADEPT-pTarget-Msp6

Plasmid

#238039

Purpose

Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.

Depositor

Lingchong You

Article

Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

Insert

sfGFP

Use

Synthetic Biology

Promoter

lac

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
ADEPT-pTarget-Msp5

Plasmid

#238038

Purpose

Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.

Depositor

Lingchong You

Article

Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

Insert

sfGFP

Use

Synthetic Biology

Promoter

lac

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4TO_24xGCN4_PCC_24xPP7

Plasmid

#236094

Purpose

Constitutively encodes 24xGCN4-PCC-24xPP7 stem loops, for visualization of the Pcc mRNA.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

24xGCN4-PCC-24xPP7

Expression

Mammalian

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4TO_24xGCN4_MUT_24xPP7

Plasmid

#236093

Purpose

Constitutively encodes 24xGCN4-MUT-24xPP7 stem loops, for visualization of the Mut mRNA.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

24xGCN4-MUT-24xPP7

Expression

Mammalian

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge