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Plasmid#75297PurposeExpresses Sacsin sr1 (SIRPT1 repeat) domain (84-339) in fusion with GST (bacterial)DepositorInsertHuman Sacsin 84-339
UseTagsGST-TagExpressionBacterialMutationPromoterAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pH7WG2B-OsMIR390-B/c
Plasmid#61465PurposePlant expression vector (OsUBI promoter) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.DepositorInsertOsMIR390-B/c
UseTagsExpressionPlantMutationO. sativa MIR390 precursor sequence including a c…PromoterOsUBIAvailable sinceMarch 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
gRNA_DNMT3b
Plasmid#41823PurposeExpresses a guide RNA (gRNA) to target DNMT3b for genome engineeringDepositorInsertgRNA_DNMT3b (DNMT3B Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAP03-act1p
Plasmid#120233PurposeCarries promoter cassette containing act1p promoter and apramycin resistance gene that can be amplified with homology sequence for refactoring.DepositorInsertpromoter cassette containing act1p promoter and apramycin resistance gene
UseSynthetic BiologyTagsExpressionMutationaac(3)IV (apramycin resistance gene)PromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
NG0750 pCI-TPI-PTC48-4H
Plasmid#65803PurposeNMD reporter mRNADepositorInsertTPI (TPI1 Human)
UseTags4H (4 copies of short beta-globin 3'UTR for …ExpressionMammalianMutationpremature stop codon at 48PromoterCMVAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P41nmt1-GFP
Plasmid#39290DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutation-1163 to +6 of nmt1 locus with a 4bp deletion (TA…PromoterAvailable sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBtPIPLC His
Plasmid#173808PurposeExpression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cellsDepositorInsertBacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
UseTags6XHisExpressionBacterialMutationnonePromoterT7Available sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKM60
Plasmid#154159PurposeFirefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, with 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutation4 wt NF-κB sites were replaced with Rel1A-specifi…PromoterAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
Brg1 Del1 pET28a
Plasmid#122115PurposeExpression Brg1 amino acids 1-381 in bacteriaDepositorInsertBrg1 (SMARCA4 Human)
UseTagsHis-T7ExpressionBacterialMutationPromoterAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P81nmt1-GFP
Plasmid#39291DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutation-1163 to +6 of nmt1 locus with a 7bp deletion (AT…PromoterAvailable sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMDC32B-OsMIR390-B/c
Plasmid#61467PurposePlant expression vector (2x35S) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.DepositorInsertOsMIR390-B/c
UseTagsExpressionPlantMutationO. sativa MIR390 precursor sequence including a c…Promoter2x35SAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCI-TPI_PTC160-xrRNA-4H
Plasmid#108369PurposeExpresses TPI reporter with premature termination codon (PTC160); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorInsertPTC160 TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
UseTagsExpressionMammalianMutationPTC160PromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMDC123SB-OsMIR390-B/c
Plasmid#61466PurposePlant expression vector (2x35S) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.DepositorInsertOsMIR390-B/c
UseTagsExpressionPlantMutationO. sativa MIR390 precursor sequence including a c…Promoter2x35SAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
ET1-human LRRC33
Plasmid#111603PurposeExpress human LRRC33 in 293T cellsDepositorInserthuman LRRC33 (NRROS Human)
UseTagsPreScission/TEV/ProteinC/6xHIS/SBP/ExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-OsMIR390-B/c
Plasmid#61468PurposeGateway-compatible entry vector for direct cloning of artificial microRNAs into rice MIR390 precursor.DepositorInsertOsMIR390-B/c
UseGateway entry vectorTagsExpressionMutationO. sativa MIR390 precursor sequence including a c…PromoterAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
DT034A
Plasmid#159395PurposeConstitutive LuxR expression, controlled under E.coli hybrid (J23117-J23106) promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoterDepositorInsertpr.J23117/J23106-LuxR-pr.LuxR-PFO-pdt
UseSynthetic BiologyTagsFLAG/FLAG and pdt#3 from Collins Nat. Biotech. 20…ExpressionBacterialMutationPromoterJ23117 and pLux promoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
NG1237 pCI-TPI-PTC160-4H
Plasmid#65804PurposeNMD reporter mRNADepositorInsertTPI (TPI1 Human)
UseTags4H (4 copies of short beta-globin 3'UTR for …ExpressionMammalianMutationpremature stop codon at 160PromoterCMVAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAA3
Plasmid#154163PurposeFirefly luciferase under the control of 5 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutation20 wt NF-κB sites were replaced with Rel1A-specif…PromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-FluoSTEP-EKAR
Plasmid#181858PurposeGenetically encoded FRET-based sensor for monitoring ERK activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.DepositorInsertFluoSTEP-EKAR
UseTagsmRuby2 and sfGFP(1-10)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
33-G-X1-pEF1-puro
Plasmid#111604PurposeExpress human 33-G-X1 in 293T cellsDepositorInserthuman LRRC33 and GARP chimeria (NRROS Human)
UseTagsFlag tagExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only