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Showing: 1601 - 1620 of 1837 results

  2. pCAG-attBr-ZsGreen-attP

    Plasmid
    #51265
    Purpose
    Bxb1-responsive fluorescent reporter plasmid containing reverse complementary ZsGreen flanked by Bxb1 attB/P recognition sites in head-to-head orientation
    Depositor
    Pawel Pelczar
    Article
    Hermann et al Nucleic Acids Res. 2014 Jan 9.
    Insert
    attBr-ZsGreen-attP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    March 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    51265-plasmid-map-sequence-id-77875

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  3. p330 VQR

    Plasmid
    #101730
    Purpose
    Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs
    Depositor
    Andrew Holland
    Article
    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi
    Insert
    SpCas9 VQR
    Use
    Tags
    3xFLAG-NLS and NLS
    Expression
    Mammalian
    Mutation
    VQR (D1135V, R1335Q, and T1337R mutations in Cas9)
    Promoter
    Available since
    Oct. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    101730-plasmid-map-sequence-id-192072

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  4. p458 VRER

    Plasmid
    #101728
    Purpose
    Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs
    Depositor
    Andrew Holland
    Article
    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi
    Insert
    SpCas9 VRER
    Use
    Tags
    3xFLAG-NLS and NLS
    Expression
    Mammalian
    Mutation
    VRER (D1135V, G1218R, R1335E, and T1337R mutation…
    Promoter
    Available since
    Oct. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    101728-plasmid-map-sequence-id-196591

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  6. pCAG-attBr-Cre-rc-attP

    Plasmid
    #51264
    Purpose
    Component of the Bxb1-rcCre binary site-specific recombinase system, reverse complementary Cre CDS flanked by Bxb1 attB/P recognition sites in head-to-head orientation
    Depositor
    Pawel Pelczar
    Article
    Hermann et al Nucleic Acids Res. 2014 Jan 9.
    Insert
    attBr-Cre-rc-attP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only
    51264-plasmid-map-sequence-id-77874

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  9. pChlr-8CQ

    Plasmid
    #52171
    Purpose
    Encodes module 8 with amino acids 12C-16Q for recognition of nt 1A
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 284-319 (PUM1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Changed 12N to 12C in module 8
    Promoter
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. pChlr-7SE

    Plasmid
    #52168
    Purpose
    Encodes module 7 with amino acids 12S-16E for recognition of nt 2G
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 241-283 (PUM1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    52168-plasmid-map-sequence-id-80997

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  11. pChlr-8SE

    Plasmid
    #52172
    Purpose
    Encodes module 8 with amino acids 12S-16E for recognition of nt 1G
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 284-319 (PUM1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S and 16Q to 16E in module 8
    Promoter
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. pChlr-1SE

    Plasmid
    #52140
    Purpose
    Encodes module 1 with amino acids 12S-16E for recognition of nt 8G
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 25-60 (PUM1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Changed 16Q to 16E in Module 1
    Promoter
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. pChlr-2SE

    Plasmid
    #52144
    Purpose
    Encodes module 2 with amino acids 12S-16E for recognition of nt 7G
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 61-96 (PUM1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S and 16Q to 16E in Module 2
    Promoter
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  14. pChlr-3SE

    Plasmid
    #52148
    Purpose
    Encodes module 3 with amino acids 12S-16E for recognition of nt 6G
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 97-132 (PUM1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Changed 12C to 12S and 16Q to 16E in Module 3
    Promoter
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. pChlr-5SR

    Plasmid
    #52162
    Purpose
    Encodes module 5 with amino acids 12S-16R for recognition of nt 4C
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 169-204 (PUM1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Changed 12C to 12S and 16Q to 16R in Module 5
    Promoter
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. pChlr-6SE

    Plasmid
    #52164
    Purpose
    Encodes module 6 with amino acids 12S-16E for recognition of nt 3G
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 205-240 (PUM1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S and 16Q to 16E in Module 6
    Promoter
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  18. pChlr-4SE

    Plasmid
    #52152
    Purpose
    Encodes module 4 with amino acids 12S-16E for recognition of nt 5G
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 133-168 (PUM1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S and 16Q to 16E in Module 4
    Promoter
    Available since
    May 23, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. pChlr-8SR

    Plasmid
    #52174
    Purpose
    Encodes module 8 with amino acids 12S-16R for recognition of nt 1C
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 284-319 (PUM1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S and 16Q to 16R in Module 8
    Promoter
    Available since
    May 23, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. pChlr-1SR

    Plasmid
    #52142
    Purpose
    Encodes module 1 with amino acids 12S-16R for recognition of nt 8C
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 25-60 (PUM1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Changed 16Q to 16R in Module 1
    Promoter
    Available since
    May 5, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 1601 - 1620 of 1837 results