Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 8,624 results for: FIE

Showing: 3241 - 3280 of 8624 results

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pFUW-TetO-PRDM1

Plasmid

#207196

Purpose

doxycycline-inducible expression of Human PRDM1 in mammalian cells

Depositor

Filipe Pereira

Article

Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

Insert

pFUW-TetO-PRDM1

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-RBPJ

Plasmid

#207197

Purpose

doxycycline-inducible expression of Human RBPJ in mammalian cells

Depositor

Filipe Pereira

Article

Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

Insert

pFUW-TetO-RBPJ

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-Stat4

Plasmid

#207199

Purpose

doxycycline-inducible expression of Human Stat4 in mammalian cells

Depositor

Filipe Pereira

Article

Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

Insert

pFUW-TetO-Stat4

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-TCF7

Plasmid

#207200

Purpose

doxycycline-inducible expression of Human TCF7 in mammalian cells

Depositor

Filipe Pereira

Article

Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

Insert

pFUW-TetO-TCF7

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-TGIF1

Plasmid

#207201

Purpose

doxycycline-inducible expression of Human TGIF1 in mammalian cells

Depositor

Filipe Pereira

Article

Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

Insert

pFUW-TetO-TGIF1

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-TRPS1

Plasmid

#207202

Purpose

doxycycline-inducible expression of Human TRPS1 in mammalian cells

Depositor

Filipe Pereira

Article

Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

Insert

pFUW-TetO-TRPS1

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUW-TetO-ARID3A

Plasmid

#207204

Purpose

doxycycline-inducible expression of Human ARID3A in mammalian cells

Depositor

Filipe Pereira

Article

Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

Insert

pFUW-TetO-ARID3A

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-ARID5A

Plasmid

#207205

Purpose

doxycycline-inducible expression of Human ARID5A in mammalian cells

Depositor

Filipe Pereira

Article

Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

Insert

pFUW-TetO-ARID5A

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-Aebp2

Plasmid

#207183

Purpose

doxycycline-inducible expression of Mouse Aebp2 in mammalian cells

Depositor

Filipe Pereira

Article

Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

Insert

pFUW-TetO-AEBP2

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-ARID5B

Plasmid

#207184

Purpose

doxycycline-inducible expression of Human ARID5B in mammalian cells

Depositor

Filipe Pereira

Article

Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

Insert

pFUW-TetO-ARID5B

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-BHLHE40

Plasmid

#207186

Purpose

doxycycline-inducible expression of Human BHLHE40 in mammalian cells

Depositor

Filipe Pereira

Article

Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

Insert

pFUW-TetO-BHLHE40

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-E2F2

Plasmid

#207187

Purpose

doxycycline-inducible expression of Human E2F2 in mammalian cells

Depositor

Filipe Pereira

Article

Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

Insert

pFUW-TetO-E2F2

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-Egr2

Plasmid

#207188

Purpose

doxycycline-inducible expression of Mouse Egr2 in mammalian cells

Depositor

Filipe Pereira

Article

Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

Insert

pFUW-TetO-EGR2

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-HLX

Plasmid

#207189

Purpose

doxycycline-inducible expression of Human HLX in mammalian cells

Depositor

Filipe Pereira

Article

Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

Insert

pFUW-TetO-HLX

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-KCNIP3

Plasmid

#207190

Purpose

doxycycline-inducible expression of Human KCNIP3 in mammalian cells

Depositor

Filipe Pereira

Article

Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

Insert

pFUW-TetO-KCNIP3

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2025

Availability

Academic Institutions and Nonprofits only

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TTR C10S/S85C/A25T

Plasmid

#238115

Purpose

bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Sun et al J Am Chem Soc. 2024 Jan 10;146(1):532-54

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S/S85C/A25T

Promoter

T7

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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TTR C10S/S85C/A120L

Plasmid

#238112

Purpose

bacterial expression of human TTR mutant

Depositor

Peter Wright

Article

Sun et al Protein Sci. 2024 Sep;33(9):e5101. doi:

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S/S85C/A120L

Promoter

T7

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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TTR C10S/S85C/A120S

Plasmid

#238113

Purpose

bacterial expression of human TTR mutant

Depositor

Peter Wright

Article

Sun et al Protein Sci. 2024 Sep;33(9):e5101. doi:

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S/S85C/A120S

Promoter

T7

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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His6-tev-G(TTR A25T/I26V)

Plasmid

#238117

Purpose

bacterial expression of human TTR mutants

Depositor

Peter Wright

Article

Sun et al J Am Chem Soc. 2024 Jan 10;146(1):532-54

Insert

TTR (TTR Human)

Tags

His6tev-G

Expression

Bacterial

Mutation

A25T/I26V

Promoter

T7

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-delta848-918

Plasmid

#238313

Purpose

ScVip1-536-1107-delta848-918 protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-delta848-918

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-R547A-H548A-R551A

Plasmid

#238317

Purpose

ScVip1-536-1107-R547A-H548A-R551A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-R547A-H548A-R551A

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-K554A-K556A-K644A

Plasmid

#238328

Purpose

ScVip1-536-1107-K554A-K556A-K644A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-K554A-K556A-K644A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pBB3-ScVip1-536-1107-P553V-G646V-G647A

Plasmid

#238322

Purpose

ScVip1-536-1107-P553V-G646V-G647A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-P553V-G646V-G647A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-K817S-D819A-S821A

Plasmid

#238326

Purpose

ScVip1-536-1107-K817S-D819A-S821A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-K817S-D819A-S821A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-TRE3G-UCP1

Plasmid

#234979

Purpose

Expresses UCP1 in mouse cell lines

Depositor

Denis Titov

Article

Choe et al Cell Chem Biol. 2025 Apr 17;32(4):620-63

Insert

UCP1 (Ucp1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.BC(p1-10)-hDLXI56i-minBG-CI-EGFP-W3SL-BC(p1-10)

Plasmid

#231360

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from the minBG promoter with hDLXI56i enhancer.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.BC(p1-10)-mscRE4-minBG-CI-EGFP-W3SL-BC(p1-10)

Plasmid

#231361

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from the minBG promoter with mscRE4 enhancer.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.minBG-EGFP-W3SL_BbsI(GGA)

Plasmid

#231362

Purpose

minBG-driven EGFP, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

EGFP

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.Ple155-CI-EGFP-W3SL_BbsI(GGA)

Plasmid

#231363

Purpose

Ple155-driven EGFP, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

EGFP

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.U6-sasgRNA(SapI)_Ple155-CI-EGFP-W3SL_BbsI(GGA)

Plasmid

#231367

Purpose

Ple155-driven EGFP, also encoding U6-driven sgRNA cassette, with SapI sites to clone crRNA sequence

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

EGFP

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.U6-sasgRNA(SapI)_Ple155-CI-mRuby2-W3SL_BbsI(GGA)

Plasmid

#231368

Purpose

Ple155-driven mRuby2, also encoding U6-driven sgRNA cassette, with SapI sites to clone crRNA sequence

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

mRuby2

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.BC(p1-10)-Ple155-CI-EGFP-W3SL-BC(p1-10)

Plasmid

#231351

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from Ple155 element, active in Purkinje cells.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.BC(p1-10)-CMVe-SCP1-TagBFP2-W3SL-BC(p1-10)

Plasmid

#231354

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses TagBFP from SCP1 promoter and CMV enhancer.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.CAG-NLS-EGFP-WPRE-SV40pA-T7-T3-BC(p1-14)

Plasmid

#231345

Purpose

Single stranded AAV genome with components for tracking AAV genome via AAV-Zombie. Also expresses a nuclear localized EGFP from CAG promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7/T3-BC(p1-14)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3_5UTR_Myc_FLuc RBM42 deletion

Plasmid

#229503

Purpose

Myc 5' untranslated region firefly luciferase reporter with deletion of region bound by RBM42.

Depositor

Davide Ruggero

Article

Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

Insert

Myc 5'UTR_Del 363

Expression

Mammalian

Mutation

Deletion of bp 363-394

Promoter

SV40

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3_5UTR_Myc_FLuc Compensatory Mutant

Plasmid

#229504

Purpose

Myc 5' untranslated region firefly luciferase reporter with compensatory mutations in the region bound by RBM42

Depositor

Davide Ruggero

Article

Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

Insert

Myc 5'UTR_CompMutant

Expression

Mammalian

Mutation

Mutations in bp 363-394

Promoter

SV40

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-eA3A-BE5-(NG)-PX458-EGFP

Plasmid

#229687

Purpose

CRISPR CBE plasmid (C to T edits): eA3A-BE5-NG engineered to include the sgRNA cloning backbone from PX458 and EGFP. Includes BbsI sites for sgRNA cloning downstream of U6 promoter.

Depositor

Deborah Marsh

Article

Dickson et al Hum Mol Genet. 2023 Jun 27:ddad105. doi:

Type

Empty backbone

Use

CRISPR

Tags

EGFP

Expression

Mammalian

Available Since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-MKI-MUT-P2A-GFP

Plasmid

#229406

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MKI-MUT-P2A-GFP

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

interaction mutant L109G; L143G (Control mutant)

Promoter

EFS

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-MKI-MUT-P2A-GFP-DOX-N

Plasmid

#229408

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MKI-MUT-P2A-GFP

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

interaction mutant L109G; L143G (Control mutant)

Promoter

TRE3G

Available Since

Dec. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-MAP4K1

Plasmid

#229396

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MAP4K1 (MAP4K1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

EFS

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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