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Showing: 1621 - 1640 of 24603 results

  2. ATP1A1_G3_EGFP_Nick_Dual_sgRNA

    Plasmid
    #178095
    Purpose
    Control vector for coselection for PE3b in human cells. Tandem expression of ATP1A1 G3 and EGFP Nick sgRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G3 + EGFP nick sgRNAs
    Use
    CRISPR; Prime editing
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Available since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178095-plasmid-map-sequence-id-350125

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  4. SIN40C.EFS.eGFP.pre

    Plasmid
    #169276
    Purpose
    Constitutive overexpression of GFP under the control of EFS promoter; for miRNA 3'UTR binding assays.
    Depositor
    Dirk Heckl
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169276-plasmid-map-sequence-id-337928

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  6. pILGFP105

    Plasmid
    #165060
    Purpose
    Integrative expression of nucleus-targeting yEGFP-AID*(mini auxin inducible degron)-CUP1
    Depositor
    Claudia Vickers
    Article
    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:
    Insert
    P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>SV40NSL>T-PGK1- T-URA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    WT
    Promoter
    Available since
    March 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    165060-plasmid-map-sequence-id-327175

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  8. Nup54_mEGFP494(-6)

    Plasmid
    #163434
    Purpose
    Mammalian expression of Nup54 with a mEGFP (missing the first 6 amino acids) at amino acid position 494
    Depositor
    Sanford Simon
    Insert
    Nup54 (NUP54 Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    163434-plasmid-map-sequence-id-321676

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  9. Nup133_mEGFP(-9b)

    Plasmid
    #163420
    Purpose
    Mammalian expression of Nup133 fused at its carboxyl-terminus to mEGFP with no linker and a total net fusion of (-9 amino acids)
    Depositor
    Sanford Simon
    Insert
    Nup133 (NUP133 Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    163420-plasmid-map-sequence-id-321639

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  10. Nup133_mEGFP(-8b)

    Plasmid
    #163418
    Purpose
    Mammalian expression of Nup133 fused at its carboxyl-terminus to mEGFP with no linker and a total net fusion of (-8 amino acids)
    Depositor
    Sanford Simon
    Insert
    Nup133 (NUP133 Human)
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    163418-plasmid-map-sequence-id-321635

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  11. pBz172_CsoS2_N1_GFP

    Plasmid
    #140852
    Purpose
    His-tagged first N-peptide of NTD fused to Superfolder GFP ([N1]-GFP) used for BLI and MST, pET-14b backbone
    Depositor
    David Savage
    Article
    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-
    Insert
    CsoS2 NTD repeat 1 fused to GFP
    Use
    Tags
    Hexahistidine tag, Strep-Tag II, and Superfolder …
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    140852-plasmid-map-sequence-id-300476

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  16. Unc104_K560GFP

    Plasmid
    #129773
    Purpose
    Expresses worm Kinesin-3 Unc104 head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP tagged
    Depositor
    William Hancock
    Insert
    Unc104 (unc-104 Nematode)
    Use
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    Truncation at 361, fused to kinesin-1 starting Al…
    Promoter
    Available since
    Nov. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    129773-plasmid-map-sequence-id-263568

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Showing: 1621 - 1640 of 24603 results