We narrowed to 31,880 results for: Eng

1. pCIFR-4

   Plasmid

   #227664

   Purpose

   Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette (Sm) used for selecting for the integration can be removed in a later stage with pFNC.

   Depositor

   Pablo Ivan Nikel

   Article

   Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

   Insert

   msfGFP
   

   Expression

   Bacterial

   Promoter

   p14G-BCD2

   Available Since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCIFR-2

   Plasmid

   #227663

   Purpose

   Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette (Km) used for selecting for the integration can be removed in a later stage with pFNC.

   Depositor

   Pablo Ivan Nikel

   Article

   Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

   Insert

   msfGFP
   

   Expression

   Bacterial

   Promoter

   p14G-BCD2

   Available Since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFNC-8

   Plasmid

   #227670

   Purpose

   Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. AmR, easily curable via sucrose counterselection.

   Depositor

   Pablo Ivan Nikel

   Article

   Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

   Insert

   AmR
   

   Expression

   Bacterial

   Available Since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFNC-4

   Plasmid

   #227668

   Purpose

   Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. SmR, easily curable via sucrose counterselection.

   Depositor

   Pablo Ivan Nikel

   Article

   Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

   Insert

   SmR
   

   Expression

   Bacterial

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. SBC015898

   Plasmid

   #226285

   Purpose

   Expresses PhCFAT, ObEGS1, and ObEOMT1 from trc promoter. Biosynthesis of methylated phenylpropenes from monolignol precursors.

   Depositor

   Rainer Breitling

   Article

   Chua et al Metab Eng Commun. 2024 May 15;18:e00237.

   Inserts

   Coniferyl alcohol acyltransferase
   Eugenol synthase 1
   Eugenol O-methyltransferase
   

   Expression

   Bacterial

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. iNicSnFR12

   Plasmid

   #227142

   Purpose

   Genetically encoded fluorescent biosensor for nicotine detection

   Depositor

   Henry Lester

   Article

   Haloi et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

   Insert

   iNicSnFR12
   

   Tags

   6xHis-HA and Myc

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTC3cIfm

   Plasmid

   #193778

   Purpose

   Expresses frame-shifted cI under the control of PLTetO-1 promoter, p15A origin of replication, Chloramphenicol selection

   Depositor

   Sangram Bagh

   Article

   Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

   Insert

   Frame-shifted CI
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PLTetO-1

   Available Since

   April 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTS2341_Tier3(SB)-Puro

   Plasmid

   #169639

   Purpose

   Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-PuroR-pA-3'ITR)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PRPBSA-driven PuroR expression
   

   Expression

   Mammalian

   Promoter

   PRPBSA

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVH616

   Plasmid

   #169605

   Purpose

   Tier-2 vector encoding PmPGK1-driven Fluc and PCREm4-driven Nluc expression (PmPGK1-Fluc-pA::A2-pA::PCREm4-Nluc-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette
   

   Expression

   Mammalian

   Promoter

   PCREm4 / PPGK

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVH270-Tier1-OTtgO2-PCMVmin2-SEAP-p2A-iRFP670

    Plasmid

    #169592

    Purpose

    Tier-1 vector encoding PTtgO2-driven SEAP-p2A-iRFP670 expression (OTtgO2-PCMVmin-2-SEAP-p2A-iRFP670-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    phloretin-controlled SEAP and iRFP expression
    

    Expression

    Mammalian

    Promoter

    TtgO2-PCMVmin-2

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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