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pCFJ150-ets4

Plasmid

#80355

Purpose

Pmex-5:GFP::H2B::PEST::ets-4 3'UTR reporter construct used for microinjection

Depositor

Sean Ryder

Article

Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

Insert

Pmex-5:GFP::H2B::PEST::ets-4 3'UTR

Expression

Worm

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCFJ150-mex3

Plasmid

#80361

Purpose

Pmex-5:GFP::H2B::PEST::mex-3 3'UTR reporter construct used for microinjection

Depositor

Sean Ryder

Article

Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

Insert

Pmex-5:GFP::H2B::PEST::mex-3 3'UTR

Expression

Worm

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCFJ150-mbk2

Plasmid

#80360

Purpose

Pmex-5:GFP::H2B::PEST::mbk-2 3'UTR reporter construct used for microinjection

Depositor

Sean Ryder

Article

Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

Insert

Pmex-5:GFP::H2B::PEST::mbk-2 3'UTR

Expression

Worm

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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TU#1805_CRISPR_unc-73_exon2

Plasmid

#82359

Purpose

to create unc-73B null allele

Depositor

Martin Chalfie

Article

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Cas9 and sgRNA against unc-73 exon2 (unc-73 Nematode)

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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TU#1449_unc-73e_cDNA

Plasmid

#82363

Purpose

express unc-73e cDNA

Depositor

Martin Chalfie

Article

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

unc-73 e isoform (unc-73 Nematode)

Expression

Worm

Promoter

none

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only
pJL1702

Plasmid

#78195

Purpose

optogenetic activation of IL2 neuron in worms

Depositor

Junho Lee

Article

Lee et al Nat Neurosci. 2011 Nov 13;15(1):107-12.

Insert

ChR2(gf)

Expression

Worm

Promoter

lag-2

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only
pJL1400

Plasmid

#78190

Purpose

Ce hsp-16.1 expression in worms

Depositor

Junho Lee

Article

Lee et al J Biol Chem. 2013 Jan 18;288(3):1582-9.

Insert

hsp-16.1 (hsp-16.1 Nematode)

Expression

Worm

Promoter

hsp-16.1

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pJL1603

Plasmid

#78193

Purpose

Ce cha-1 expression in SMB, SIA neurons in worms

Depositor

Junho Lee

Article

Lee et al Nat Neurosci. 2011 Nov 13;15(1):107-12.

Insert

cha-1 (cha-1 Nematode)

Expression

Worm

Promoter

odr-2

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pJL580

Plasmid

#78185

Purpose

Ce rpy-1 expression in worms

Depositor

Junho Lee

Article

Nam et al J Biol Chem. 2009 Mar 20;284(12):8195-20

Insert

rpy-1 (rpy-1 Nematode)

Expression

Worm

Promoter

rpy-1

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pJL1602

Plasmid

#78192

Purpose

Ce cha-1 expression in SIA, RMD neurons in worms

Depositor

Junho Lee

Article

Lee et al Nat Neurosci. 2011 Nov 13;15(1):107-12.

Insert

cha-1 (cha-1 Nematode)

Expression

Worm

Promoter

trp-1

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pJL1601

Plasmid

#78191

Purpose

Ce cha-1 expression in IL2, SIA neurons in worms

Depositor

Junho Lee

Article

Lee et al Nat Neurosci. 2011 Nov 13;15(1):107-12.

Insert

cha-1 (cha-1 Nematode)

Expression

Worm

Promoter

osm-6

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only
pSR13

Plasmid

#69155

Purpose

read-outloxN mCherry to GFP switch, with swsn-1 promoter, gene (partially cDNA) and UTR, for integration on on ttTi5605, Mos Chr II

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Inserts

mCherry
swsn-1 (swsn-1 Nematode)
eGFP

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0, codon-optimzed index 1.…

Promoter

rps-27 and swsn-1

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pSR11

Plasmid

#69154

Purpose

read-outloxP mCherry to GFP switch, with fzr-1 promoter, gene and UTR, for integration on cxtTi10816, Mos Chr IV

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Inserts

mCherry
fzr-1 (fzr-1 Nematode)
eGFP

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0

Promoter

fzr-1 and rps-27

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pKPY737

Plasmid

#72673

Purpose

Encodes Thr412Gly-CePheRS Alpha Subunit (Gateway Entry Clone)

Depositor

David Tirrell

Insert

Thr412Gly-CePheRS Alpha Subunit::C. elegans CEOP5428 Intercistronic Region::HA-GFP [From pPD95.75 (+118 to +1000)] in pDONR221

Use

Gateway entry clone

Mutation

Thr412Gly in CePheRS Alpha Subunit

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pGC461

Plasmid

#71363

Purpose

distal tip cell expression of DAF-16 in C. elegans

Depositor

Jane Hubbard

Article

Michaelson et al Development. 2010 Feb;137(4):671-80. doi

Insert

daf-16 (daf-16 Nematode)

Tags

GFP(65C)

Expression

Worm

Promoter

Blelloch et al., 1999; Mathies et al., 2003

Available Since

Dec. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pClneoMyc'-cYAP-1

Plasmid

#66854

Purpose

Express Myc-tagged C.elegans YAP-1 in mammalian cells

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

Insert

YAP-1 (yap-1 Nematode)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pClneoMyc'-EGL-44

Plasmid

#66855

Purpose

Express Myc-tagged C.elegans EGL-44 in mammalian cells

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

Insert

EGL-44 (CELE_F28B12.2 Nematode)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pClneoEGFPC-let60 K16N

Plasmid

#66861

Purpose

Express GFP-tagged dominat negative let60, in which Lysine 16 is mutated to asparagine, in mammalian cells

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Feb 1;319(3):1-11. do

Insert

Let-60 (let-60 Nematode)

Tags

GFP

Expression

Mammalian

Mutation

Lysine 16 is mutated to asparagine

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pPD95.75 RSF-1

Plasmid

#66863

Purpose

Generate transgenic C..elegans expressing GFP-fused RSF-1

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Feb 1;319(3):1-11. do

Insert

RSF-1 (rsf-1 Nematode)

Tags

GFP

Expression

Worm

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
RGS-6xHis-LIN-29-pcDNA3.1-

Plasmid

#52515

Purpose

expresses C. elegans LIN-29 in mammalian cells with RGS-6xHis tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

Lin-29 b (lin-29 Nematode)

Tags

RGS-6xHis

Expression

Mammalian

Mutation

wt (changed coding sequence in 5' (5th codon…

Promoter

CMV

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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