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Showing: 2461 - 2490 of 74627 results

  6. SNV-memTdTomato-2AcytoGFP

    Plasmid
    #53124
    Purpose
    Retrovirus vector backbone that co-expresses GFP and a gene of interest fused to memTdTomato, driven by the Spleen Necrosis Virus promoter; for use in chicken cells
    Depositor
    Jeanette Hyer
    Article
    Venters et al Dev Dyn. 2008 Nov;237(11):3260-9. doi: 1
    Type
    Empty backbone
    Use
    Retroviral; Expression in chicken cells
    Tags
    2A-cyto-GFP and memTdTomato
    Expression
    Mutation
    Promoter
    SNV (spleen necrosis virus)
    Available since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only

  7. pcDNA3.1 Flag YFP hNKCC1 A720C C723S C724V (NT534)

    Plasmid
    #50899
    Purpose
    Expresses human NKCC1 A720C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    A720C C723S C724V in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50899-plasmid-map-sequence-id-76372

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  8. pcDNA3.1 Flag YFP hNKCC1 L718C C723S C724V (NT532)

    Plasmid
    #50897
    Purpose
    Expresses human NKCC1 L718C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    L718C C723S C724V in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50897-plasmid-map-sequence-id-76370

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  9. pcDNA3.1 Flag YFP hNKCC1 L722C C723S C724V (NT536)

    Plasmid
    #50901
    Purpose
    Expresses human NKCC1 L722C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    L722C C723S C724V in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50901-plasmid-map-sequence-id-76374

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  10. pcDNA3.1 Flag YFP hNKCC1 S716C C723S C724V (NT530)

    Plasmid
    #50895
    Purpose
    Expresses human NKCC1 S716C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    S716C C723S C724V in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50895-plasmid-map-sequence-id-76368

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  11. pcDNA3.1 Flag YFP hNKCC1 I721C C723S C724V (NT535)

    Plasmid
    #50900
    Purpose
    Expresses human NKCC1 I721C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    I721C C723S C724V in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50900-plasmid-map-sequence-id-76373

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  12. pcDNA3.1 Flag YFP hNKCC1 C723S C724V W732C (NT546)

    Plasmid
    #50908
    Purpose
    Expresses human NKCC1 C723S C724V W732C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    C723S C724V W732C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50908-plasmid-map-sequence-id-76381

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  13. pcDNA3.1 Flag YFP hNKCC1 C723S C724V I725C (NT538)

    Plasmid
    #50903
    Purpose
    Expresses human NKCC1 C723S C724V I725C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    C722S C723V I724C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50903-plasmid-map-sequence-id-76376

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  14. pcDNA3.1 Flag YFP hNKCC1 C723S C724V V726C (NT539)

    Plasmid
    #50904
    Purpose
    Expresses human NKCC1 C723S C724V V726C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    C723S C724V V726C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50904-plasmid-map-sequence-id-76377

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  15. pcDNA3.1 Flag YFP hNKCC1 G719C C723S C724V (NT533)

    Plasmid
    #50898
    Purpose
    Expresses human NKCC1 G719C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    G719C C723S C724V in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50898-plasmid-map-sequence-id-76371

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  16. pcDNA3.1 Flag YFP hNKCC1 N-terminal deletion (NT53)

    Plasmid
    #49062
    Purpose
    Expresses human NKCC1 truncation mutant (aa13-222) with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    N-terminal deletion of amino acids 13-222 in hNKC…
    Promoter
    CMV
    Available since
    Nov. 1, 2013
    Availability
    Academic Institutions and Nonprofits only
    49062-plasmid-map-sequence-id-72184

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  20. pCJR01

    Plasmid
    #187888
    Purpose
    Escherichia coli plasmid with cloned regions of homology to Campylobacter jejuni chromosome flanking a NotI restriction site. pCJR01 functions as a suicide vector for integrating cloned NotI DNA.
    Depositor
    Gaylen Uhlich
    Article
    Uhlich et al Biotechniques. 2022 Apr 13. doi: 10.2144
    Insert
    DNA insert has homology to a rRNA region of the Campylobacter jejuni chromosome with an added central NotI restriction site
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    none
    Available since
    Aug. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    187888-plasmid-map-sequence-id-368412

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  22. M13-kmR

    Plasmid
    #206861
    Purpose
    M13KE from NEB modified with kanamycin resistance gene and NotI restriction site on g3p ( M13KE-NotI-Kan)
    Depositor
    Irene Chen
    Article
    Lin et al PLoS One. 2011;6(5):e19991. doi: 10.1371
    Insert
    Kanamycin resistance gene
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lac
    Available since
    Nov. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    206861-plasmid-map-sequence-id-412778

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  23. mycBioID2-13X Linker-MCS

    Plasmid
    #92308
    Purpose
    To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a 25nm GS linker are present downstream of BioID2 and myc tags.
    Depositor
    Kyle Roux
    Article
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    Humanized A. aeolicus BioID2 with R40G and Myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    92308-plasmid-map-sequence-id-216421

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  29. pCMV6-AC EJ7-GFP

    Plasmid
    #113617
    Purpose
    Reporter for canonical-NHEJ. Reporter for end joining between two double-strand breaks, induced with the 7a and 7b sgRNAs with CAS9. EJ between the distal ends w/o indel mutations restores GFP
    Depositor
    Jeremy Stark
    Article
    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:
    Insert
    EJ7-GFP variant of EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    113617-plasmid-map-sequence-id-218254

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Showing: 2461 - 2490 of 74627 results