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We narrowed to 2,481 results for: D10

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Showing: 1641 - 1660 of 2481 results

  8. Control click editor - pCMV-T7-PCV2-deadCas9-EcKlenow (EMK500)

    Plasmid
    #208944
    Purpose
    A control CE1 construct with catalytically inactivated SpCas9, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCV2-XTEN-dSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    dSpCas9(D10A/H840A);EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    208944-plasmid-map-sequence-id-415672

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  9. FRIG-myc-Sema4D-CD4

    Plasmid
    #51606
    Purpose
    expresses myc-tagged Sema4D/CD4 non-cleavable chimera with extracellular Sema4D, intracellular CD4 in lentiviral backbone
    Depositor
    Suzanne Paradis
    Publication
    Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do
    Insert
    Sema4D/CD4-FLAG (Sema4d Mouse, Human)
    Use
    Lentiviral
    Tags
    FLAG and myc
    Expression
    Mammalian
    Mutation
    Sema4D amino acids 660-861 were replaced with CD4…
    Promoter
    RSV
    Available since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. pFB1.HMBP-Prs.AEBP2iso2.mt2K

    Plasmid
    #247010
    Purpose
    Expresses human AEBP2iso2.mt2K (with twelve asp/glu-to-lys mutations) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.
    Depositor
    Chen Davidovich
    Publication
    Mucha et al EMBO J. 2025 Dec;44(23):6979-7020. doi:
    Insert
    AEBP2iso2.mt2K (AEBP2 Human)
    Tags
    6xHis-MBP
    Expression
    Insect
    Mutation
    E95K, D96K, E97K, D98K, E99K, E100K, E101K, D102K…
    Promoter
    Polyhedrin
    Available since
    Nov. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    247010-plasmid-map-sequence-id-493366

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  11. pFB1.HMBP-Prs.AEBP2iso2.mt2A

    Plasmid
    #247009
    Purpose
    Expresses human AEBP2iso2.mt2A (with fifteen asp/glu-to-ala mutations) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.
    Depositor
    Chen Davidovich
    Publication
    Mucha et al EMBO J. 2025 Dec;44(23):6979-7020. doi:
    Insert
    AEBP2iso2.mt2A (AEBP2 Human)
    Tags
    6xHis-MBP
    Expression
    Insect
    Mutation
    E95A, D96A, E97A, D98A, E99A, E100A, E101A, D102A…
    Promoter
    Polyhedrin
    Available since
    Nov. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    247009-plasmid-map-sequence-id-493364

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  16. dCas9-CBP HAT

    Plasmid
    #179547
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human CBP HAT domain (aa 1323-1701) driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Publication
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of human CBP HAT domain (aa 1323-1701) (CREBBP Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179547-plasmid-map-sequence-id-354768

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  17. pFB1.HMBP-Prs.AEBP2iso2.mt1K2K

    Plasmid
    #247013
    Purpose
    Expresses human AEBP2iso2.mt1K2K (with 27 asp/glu-to-lys mutations) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.
    Depositor
    Chen Davidovich
    Publication
    Mucha et al EMBO J. 2025 Dec;44(23):6979-7020. doi:
    Insert
    AEBP2iso2.mt1K2K (AEBP2 Human)
    Tags
    6xHis-MBP
    Expression
    Insect
    Mutation
    E37K, E38K, E39K, E40K, E41K, E42K, E43K, E44K, E…
    Promoter
    Polyhedrin
    Available since
    Nov. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    247013-plasmid-map-sequence-id-493367

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  18. pFB1.HMBP-Prs.AEBP2iso2.mt1A2A

    Plasmid
    #247012
    Purpose
    Expresses human AEBP2iso2.mt1A2A (with 27 asp/glu-to-ala mutations) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.
    Depositor
    Chen Davidovich
    Publication
    Mucha et al EMBO J. 2025 Dec;44(23):6979-7020. doi:
    Insert
    AEBP2iso2.mt1A2A (AEBP2 Human)
    Tags
    6xHis-MBP
    Expression
    Insect
    Mutation
    E37A, E38A, E39A, E40A, E41A, E42A, E43A, E44A, E…
    Promoter
    Polyhedrin
    Available since
    Nov. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    247012-plasmid-map-sequence-id-493365

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  19. pCMV-T7-TadCBEd-SpCas9(LWKYQS)-P2A-EGFP (BKS1014)

    Plasmid
    #223127
    Purpose
    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized TadA-CDd-SpCas9(LWKYQS)-2xUGI-P2A-EGFP
    Use
    CRISPR
    Tags
    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd
    Expression
    Mammalian
    Mutation
    TadA-CDd mutations; nSpCas9(D10A/LWKYQS)=D1135L, …
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223127-plasmid-map-sequence-id-447695

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  20. pCMV-T7-ABE8e-SpCas9(LWRYEK)-P2A-EGFP (BKS1028)

    Plasmid
    #223114
    Purpose
    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with LWRYEK amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized TadA8e-SpCas9(LWRYEK)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-P2A-EGFP and BPNLS-TadA8e
    Expression
    Mammalian
    Mutation
    TadA8e mutations; nSpCas9(D10A/LWRYEK)=D1135L, S1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223114-plasmid-map-sequence-id-447638

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Showing: 1641 - 1660 of 2481 results