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pDEST-GST-FBW7 alpha

Plasmid

#197456

Purpose

The plasmid expresses GST-tagged FBW7 human isoform 1. Used for in vitro translation of FBW7alpha.

Depositor

Omar Khan

Article

Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

Insert

FBW7 (FBXW7 Human)

Use

In vitro translation

Tags

GST

Expression

Bacterial

Mutation

See depositor comments below

Promoter

T7

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMuLE Lenti Dest iRFP

Plasmid

#62176

Purpose

MuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses iRFP under the control of a PGK promoter. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

Lentiviral; Mule gateway destination vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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362 pCS Cherry DEST

Plasmid

#13075

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

Cherry

Use

Tags

Expression

Mutation

Promoter

Available since

June 18, 2010

Availability

Academic Institutions and Nonprofits only

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pDEST-mCherry-LacR-BRCA1

Plasmid

#71115

Purpose

mammalian expression of mCherry-LacR-BRCA1 siRNA resistant construct

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

BRCA1 (BRCA1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV/TO

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST40-2XFL-Src-K298M

Plasmid

#140315

Purpose

Expresses Flag-tagged human Src-K298M in mammalian cells for structure-function studies

Depositor

Mark Moasser

Article

Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

Insert

Src mutant (SRC Human)

Use

Tags

2X Flag

Expression

Mammalian

Mutation

K298M

Promoter

CMV

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMuLE Lenti Dest Neo

Plasmid

#62178

Purpose

MuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses Neomycin cassette under the control of a PGK promoter. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

Lentiviral; Mule gateway destination vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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LB901: pMVP/PB/Blast-DEST

Plasmid

#121873

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ Blast selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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223 pCS EGFP DEST

Plasmid

#13071

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

EGFP

Use

Tags

Expression

Mutation

Promoter

Available since

June 18, 2010

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/FLAG/HA-DEST PABPC4

Plasmid

#19882

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Oct 31. ():.

Insert

Poly(A) binding protein, cytoplasmic 4 (PABPC4 Human)

Use

Tags

FLAG/HA

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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IZ501: pMVP/Lenti-DEST

Plasmid

#121848

Purpose

pMVP destination vector, empty Lentivirus vector backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Lentiviral; Pmvp gateway destination vector

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pFRT/FLAG/HA-DEST QKI

Plasmid

#19891

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Oct 31. ():.

Insert

quaking homolog, KH domain RNA binding (QKI Human)

Use

Tags

FLAG/HA

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pDEST-mCherry-LacR-PALB2

Plasmid

#71112

Purpose

mammalian expession of mCherry-LacR-PALB2

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

PALB2 (PALB2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV/TO

Available since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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JN302: pMVP/SB/Puro-DEST

Plasmid

#121865

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Puro selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2/V5-DEST MTFR1L-V5

Plasmid

#69822

Purpose

Expresses MTFR1L-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Mitochondrial fission regulator 1-like (MTFR1L Human)

Use

Retroviral

Tags

v5

Expression

Mammalian

Mutation

Promoter

cmv

Available since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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LB701: pMVP/PB/mCherry-DEST

Plasmid

#121871

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ mCherry selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pQCXIH CMV/TO DEST (w382-1)

Plasmid

#17394

Purpose

Retroviral Gateway destination vector, Expression, CMV/TO promoter, Hygro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Retroviral; Destination vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti X2 Zeo DEST (749-3)

Plasmid

#21562

Purpose

3rd gen lentiviral Gateway destination vector, RNAi, 3'LTR insertion, Zeo

Depositor

Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA pDEST40 Cas9-POLD3

Plasmid

#183194

Purpose

Expression clone

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Insert

POLD3

Use

CRISPR

Tags

Cas9WT

Expression

Mammalian

Mutation

n/a

Promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pFRT/FLAG/HA-DEST EIF2C2

Plasmid

#19888

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Oct 31. ():.

Insert

Eukaryotic translation initiation factor 2C, 2 (AGO2 Human)

Use

Tags

FLAG/HA

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pDestTol2pA2-blasticidin--3.5ubb:plin2-tdtomato

Plasmid

#172535

Purpose

Zebrafish ubiquitin promoter driving zebrafish PLIN2-tdTOMATO fusion (lipid droplet reporter) with em7:bsd for blasticidin resistance

Depositor

Richard White

Article

Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

Insert

-3.5ubb:plin2-tdtomato

Use

Tags

Expression

Mutation

S80R, A81S, E144D, I148F and R415H mutations obse…

Promoter

zebrafish ubb

Available since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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