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pCMK103

Plasmid

#226803

Purpose

Integrates KanR into NS1, 500 bp homology, one homologous HIP1 site in left homology arm

Depositor

David Nielsen

Article

Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCMK104

Plasmid

#226804

Purpose

Integrates KanR into NS2, 500 bp homology, one homologous HIP1 site in right homology arm

Depositor

David Nielsen

Article

Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pCMK105

Plasmid

#226805

Purpose

Integrates KanR into glpK, 570 bp homology, three homologous HIP1 sites in left homology arm

Depositor

David Nielsen

Article

Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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GH-t2a GFP

Plasmid

#194883

Purpose

an overexpression vector of the killifish growth hormone

Depositor

Itamar Harel

Article

Moses et al eLife. 2023 Oct 24;12:e85960. doi: 10.75

Insert

growth hormone

Use

Lentiviral

Tags

t2a-GFP

Promoter

CMV

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pBEST-PrOR1OR2-MGapt-UTR1-deGFP

Plasmid

#227648

Purpose

PrOR1OR2-driven expression of MGapt and deGFP for quantifying cell-free RNA and protein synthesis.

Depositor

Richard Murray

Article

Kapasiawala et al bioRxiv 2023.03.22.533877

Insert

MG aptamer (MGapt) and deGFP

Use

Synthetic Biology

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pBEST-PTet-MGapt-UTR1-deGFP

Plasmid

#227647

Purpose

pTet-driven expression of MGapt and deGFP for quantifying cell-free RNA and protein synthesis.

Depositor

Richard Murray

Article

Kapasiawala et al bioRxiv 2023.03.22.533877

Insert

MG aptamer (MGapt) and deGFP

Use

Synthetic Biology

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pJL1-V3-FETCH1

Plasmid

#210650

Purpose

In vitro expression of V3-FETCH reporter from the T7 promoter

Depositor

Michael Jewett

Article

Willi et al ACS Synth Biol. 2024 Jul 19;13(7):2253-2

Insert

V3-FETCH1

Use

Synthetic Biology

Promoter

T7

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pJL1-FETCH2

Plasmid

#210645

Purpose

In vitro expression of FETCH reporter from the T7 promoter

Depositor

Michael Jewett

Article

Willi et al ACS Synth Biol. 2024 Jul 19;13(7):2253-2

Insert

FETCH2

Use

Synthetic Biology

Promoter

T7

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-4

Plasmid

#221490

Purpose

NKX2-4 lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-4 (NKX2-4 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-4

Plasmid

#221478

Purpose

Gateway cloning of NKX2-4 overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-4 (NKX2-4 Human)

Use

Gateway donor vector

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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