We narrowed to 31,880 results for: Eng

1. pVH266-Tier1-OTetO7-PCMVmin2-SEAP-sTRSV

   Plasmid

   #169589

   Purpose

   Tier-1 vector encoding PTetO7-driven SEAP expression with a 3'UTR sTRSV ribozyme (OTetO7-PCMVmin-2-SEAP-3'UTRsTRSV-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   tetracycline-controlled SEAP expression
   

   Expression

   Mammalian

   Promoter

   TetO7-PCMVmin-2

   Available Since

   July 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTS2393-Tier1-PhCMV-p2a_fastFT

   Plasmid

   #169530

   Purpose

   Tier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "fast" maturation fastFT fused to a "self-cleaving" peptide p2a (PhCMV-p2a_medFT-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven P2A-fused fluorescent timer, fast maturation time
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   July 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pVH1001-Tier2(ColE1 ori)

   Plasmid

   #169599

   Purpose

   Tier-2 expression vector (A1-pA::A2-pA::A3-pA). The tier-2 expression vector consists of a custom MCS cassette containing the three Tier-1 compatible acceptor cassettes (A1, A2, and A3) each with its own non-homologous polyadenylation (pA) signals in a minimal backbone (AmpR and ColE1 ori).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   July 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTS2351_Tier3(SB)-Zeo

   Plasmid

   #169648

   Purpose

   Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a ZeoR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-ZeoR-pA-3'ITR)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PRPBSA-driven ZeoR expression
   

   Expression

   Mammalian

   Promoter

   PRPBSA

   Available Since

   July 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVH614

   Plasmid

   #169603

   Purpose

   Tier-2 vector encoding PmPGK1-driven Fluc and PCREm4-driven Nluc expression (PmPGK1-Fluc-pA::PCREm4-Nluc-pA::A3-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette
   

   Expression

   Mammalian

   Promoter

   PCREm4 / PPGK

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVH267-Tier1-OTetO7-PCMVmin2-SEAP-env140

   Plasmid

   #169590

   Purpose

   Tier-1 vector encoding PTetO7-driven SEAP expression with a 3'UTR env140 ribozyme (OTetO7-PCMVmin-2-SEAP-3'UTRenv140-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   tetracycline-controlled SEAP expression
   

   Expression

   Mammalian

   Promoter

   TetO7-PCMVmin-2

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTS2392-Tier1-PhCMV-p2a_medFT

   Plasmid

   #169529

   Purpose

   Tier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "medium" maturation medFT fused to a "self-cleaving" peptide p2a (PhCMV-p2a_medFT-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven P2A-fused fluorescent timer, medium maturation time
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTS1046-Tier1-PhCMV-4xCox8-mRuby2

   Plasmid

   #169537

   Purpose

   Tier-1 vector encoding PhCMV-driven mRuby2 localized to the mitochondrial matrix via a four-fold Cox8 localization signal (PhCMV-4xCox8-mRuby2-pA)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven miochondria-localized monomeric red fluorescent protein mRuby2
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVH269-Tier1-OVanO2-PCMVmin2-SEAP-p2A-iRFP670

   Plasmid

   #169667

   Purpose

   Tier-1 vector encoding PVanO2-driven SEAP-p2A-iRFP670 expression (OVanO2-PCMVmin-2-SEAP-p2A-iRFP670-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   vanillic acid-controlled SEAP and iRFP expression
   

   Expression

   Mammalian

   Promoter

   VanO2-PCMVmin

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVH321-2-Tier1-PhCMV-NLS-dCas9-3xNLS

    Plasmid

    #169596

    Purpose

    Tier-1 vector encoding PhCMV-driven NLS-dCas9-3xNLS expression (PhCMV-NLS-dCas9-3xNLS-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    dead S.pyogenes Cas9
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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