Skip to main content
Addgene
Showing: 4151 - 4200 of 4899 results

  2. AA427

    Plasmid
    #215961
    Purpose
    Fragmid fragment: (guide cassette) enables iBar UMIs
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; BsmBI_v0; BsmBI_v5; trRNA_v6 [Sp]
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215961-plasmid-map-sequence-id-431415

    Enlarge

  4. pShCAST(Cas12k-TniQ)-sgRNA_entry (CJT11)

    Plasmid
    #181787
    Purpose
    Expresses 3-component ShCAST containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    ShTnsB, ShTnsC, ShCas12k-ShTniQ
    Use
    Tags
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    181787-plasmid-map-sequence-id-354706

    Enlarge

  6. pEasyG3_nat

    Plasmid
    #184918
    Purpose
    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_nat recombines in vivo with a PCR product from pEasyG3_mic.
    Depositor
    Jeferson Gross
    Article
    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184918-plasmid-map-sequence-id-368408

    Enlarge

  7. pEasyG3_mic

    Plasmid
    #184920
    Purpose
    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_mic recombines in vivo with a PCR product from pEasyG3_zeo/nat/hph.
    Depositor
    Jeferson Gross
    Article
    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184920-plasmid-map-sequence-id-368413

    Enlarge

  8. pIK208

    Plasmid
    #65630
    Purpose
    sgRNA targeting dpy-10 (Arribere et al., 2014) expressed from a C. elegans U6 promoter (Friedland et al., 2013), in a "flipped and extended" sgRNA backbone (Chen et al., 2013)"
    Depositor
    Iskra Katic
    Article
    Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0
    Insert
    U6 promoter::dpy-10 sgRNA with a "flipped and extended" sgRNA backbone
    Use
    CRISPR
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    June 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    65630-plasmid-map-sequence-id-111541

    Enlarge

  9. pGS1034

    Plasmid
    #63796
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal CBP-3xFLAG-TEV-ProtA triple tag and a LEU2 selection marker
    Depositor
    Bjoern Sander, Monika Golas
    Article
    Lin et al Biochim Biophys Acta. 2015 Mar;1854(3):1
    Insert
    CBP-3xFLAG-TEV-Protein A followed by KlLEU2
    Use
    E. coli cloning vector
    Tags
    CBP-3xFLAG-TEV-Protein A
    Expression
    Mutation
    Promoter
    none
    Available since
    April 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    63796-plasmid-map-sequence-id-110311

    Enlarge

  12. pPPC001

    Plasmid
    #171138
    Purpose
    For integration of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method
    Depositor
    Jesse Zalatan
    Article
    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(
    Insert
    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    SoxS has R93A and S101A mutations
    Promoter
    Sp.pCas9, BBa_J23107
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    171138-plasmid-map-sequence-id-344761

    Enlarge

  14. pGS1022

    Plasmid
    #59576
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a URA3 selection marker
    Depositor
    Bjoern Sander, Monika Golas
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    Twin-Strep tag optimized for PCR amplification followed by URA3 selection marker
    Use
    E. coli cloning vector
    Tags
    Twin-Strep tag
    Expression
    Mutation
    Promoter
    None
    Available since
    Aug. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    59576-plasmid-map-sequence-id-92983

    Enlarge

  15. ATP1A1_G8_Dual_sgRNA

    Plasmid
    #178104
    Purpose
    Coselection for PE3 in human cells. Vector for tandem expression of ATP1A1 G8 sgRNA with a user-specified PE3 nick sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G8 sgRNA + user-specified sgRNA
    Use
    CRISPR; Prime editing
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Available since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178104-plasmid-map-sequence-id-350193

    Enlarge

  17. kiCAP-AAV-M.Mus.1

    Plasmid
    #196681
    Purpose
    Rep/Cap plasmid for the production of M.Mus.1, an AAV capsid with CNS tropism in mice.
    Depositor
    Pardis Sabeti
    Article
    Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    WVLPSGG insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    April 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196681-plasmid-map-sequence-id-389742

    Enlarge

  18. kiCAP-AAV-MDV1B

    Plasmid
    #196680
    Purpose
    Rep/Cap plasmid for the production of MDV1B, an AAV capsid with CNS tropism in mice.
    Depositor
    Pardis Sabeti
    Article
    Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    KTVGTVY insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    196680-plasmid-map-sequence-id-386956

    Enlarge

  19. attB‐mCh­‐Poly(A)-­PuroR

    Plasmid
    #65529
    Purpose
    PuroR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.
    Depositor
    Sebastian Bultmann
    Article
    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv
    Insert
    attB-mCherry
    Use
    Mouse Targeting; Bxb1
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    65529-plasmid-map-sequence-id-111228

    Enlarge

  20. pEasyG2_mic

    Plasmid
    #184916
    Purpose
    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_mic recombines in vivo with a PCR product from pEasyG2_zeo/nat/hph.
    Depositor
    Jeferson Gross
    Article
    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-
    Insert
    gRNA scaffold
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184916-plasmid-map-sequence-id-371408

    Enlarge

  22. ATP1A1_G8_MTOR_Nick_Exon-53_Dual_sgRNA

    Plasmid
    #178107
    Purpose
    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G8 and MTOR Nick exon-53 sgRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G8 + MTOR nick exon-53 sgRNAs
    Use
    CRISPR; Prime editing
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Available since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178107-plasmid-map-sequence-id-350194

    Enlarge

  24. pGfa28-nLac

    Plasmid
    #53130
    Purpose
    Expression of LacZ driven by partial human gfa2 promoter, expression is localized to a subset of astrocytes in mice.
    Depositor
    Michael Brenner
    Article
    Lee et al Glia. 2006 May;53(7):677-87.
    Insert
    Gfa2 (GFAP Human)
    Use
    Mouse Targeting
    Tags
    LacZ, NLS, and mP1
    Expression
    Mammalian
    Mutation
    contains the A and B regions (bp -1757 to -1489) …
    Promoter
    human Gfa2
    Available since
    July 30, 2014
    Availability
    Academic Institutions and Nonprofits only
    53130-plasmid-map-sequence-id-82730

    Enlarge

  26. pRP(Exp)-CMV> ORF_924bp (Azgp1):dTomato:IRES:Puro

    Plasmid
    #110831
    Purpose
    Expresses both zinc alpha-2 glycoprotein (ZAG) and dTomato (dT) reporter, where the fluorescent dT reporter is fused with the ZAG protein.
    Depositor
    Ande Satyanarayana
    Article
    Elattar et al FASEB J. 2018 Mar 23:fj201701465RR. doi:
    Insert
    Azgp1 (Azgp1 Mouse)
    Use
    Non-viral gene expression vector
    Tags
    dTomato
    Expression
    Mutation
    Promoter
    CMV
    Available since
    June 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    110831-plasmid-map-sequence-id-214111

    Enlarge

  28. ATP1A1_G3_RUNX1_Nick-38_Dual_sgRNA

    Plasmid
    #173214
    Purpose
    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and RUNX1 nick-38 sgRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G3 sgRNA + RUNX1 Nick-38 sgRNA
    Use
    CRISPR; Prime editing (pe3)
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173214-plasmid-map-sequence-id-339507

    Enlarge

  31. pYI2206

    Plasmid
    #228348
    Purpose
    Constitutive rPhlTA mutant expression
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    phlTA mutant
    Use
    Synthetic Biology
    Tags
    Three copies of VP16 (VP48)-Nuclear localization …
    Expression
    Yeast
    Mutation
    P5S, S6P, K86T, F109L, Q117R, E143K
    Promoter
    KpGAPDH promoter
    Available since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228348-plasmid-map-sequence-id-457096

    Enlarge

  32. pMT1026

    Plasmid
    #228299
    Purpose
    Constitutive expression of PhlTA mutant with weak promoter and terminator
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    phlTA mutant
    Use
    Synthetic Biology
    Tags
    Three copies of VP16 (VP48)-Nuclear localization …
    Expression
    Yeast
    Mutation
    E41G
    Promoter
    KpYPT1 promoter
    Available since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228299-plasmid-map-sequence-id-457081

    Enlarge

  33. pMT478

    Plasmid
    #228286
    Purpose
    Constitutive mutant rPhlTA expression with strong promoter
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    rphlTA mutant
    Use
    Synthetic Biology
    Tags
    Three copies of VP16 (VP48)-Nuclear localization …
    Expression
    Yeast
    Mutation
    P5S, S6P, K86T, F109L, Q117R, E143K
    Promoter
    KpGAPDH promoter
    Available since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228286-plasmid-map-sequence-id-457102

    Enlarge

  48. pDM097

    Plasmid
    #216819
    Purpose
    Aspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for N-terminal or internal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mStayGold
    Use
    Tags
    FLAG-(SGGS)x2-XTEN16-(GGGGS)x3 and c4-(GGGGS)x2-X…
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216819-plasmid-map-sequence-id-430144

    Enlarge

  49. pILGFPEasy9R

    Plasmid
    #218291
    Purpose
    Plasmid-free in-situ promoter cloning and characterisation system in S. cerevisiae, allows the characterisation of a bidirectional promoter using yEGFP and E2-Crimson as reporters.
    Depositor
    Claudia Vickers
    Article
    McDonnell et al bioRxiv 2024.03.26.586389
    Insert
    pURA3>KlURA3>tKlURA3-tPGK1KanMX>tAgTEF-pMAL32>MazF(RPL28i)-E2Crimson>tPDC1-pDDI2>ISceI>tSynth3>ura3(4,191)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218291-plasmid-map-sequence-id-433736

    Enlarge

  50. pToxAmp21R

    Plasmid
    #218287
    Purpose
    A complete plasmid for ToxAmp (Toxin-antitoxin-driven gene amplification) for the expression of AeBlue
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    HO(-955, -789)-LoxP>pKlLEU2>KlLEU2>PCRT1>RelB>tPDC1-pTDH3>AeBlue>tSYNth7-ARS712-HO(-731, -264)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218287-plasmid-map-sequence-id-433689

    Enlarge

Showing: 4151 - 4200 of 4899 results