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Plasmid#171620PurposeM13-TevC with CreDepositorInsertM13-TevC-IRES-SP6-Cre
UseAAV and Synthetic BiologyTags6x HisExpressionMammalianMutationPromoterhSynAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-TA Golgin84-IRES-Puromycin
Plasmid#133028PurposeExpresses EGFP-tagged Golgi apparatus markerDepositorInsertGolgin84 (GOLGA5 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationdeleted amino acids 1-673PromoterHuman elongation factor 1 alphaAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1alpha-nCoV2019-N-meGFP-IRES-Puro
Plasmid#185451PurposeExpresses SARS-CoV-2 nucleocapsid protein with a c-terminal monomeric EGFP in mammalian cellsDepositorInsertNucleocapsid (N SARS-CoV-2)
UseLentiviralTagsmEGFPExpressionMammalianMutationPromoterEF1alphaAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV-BCR-ABL1-p190-IRES-EGFP
Plasmid#219964PurposeExpress human BCR-ABL1-P190 in mammalian cellsDepositorInsertBCR-ABL1-P190 (ABL1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-ERGIC53-IRES-Puromycin
Plasmid#134859PurposeExpresses EGFP-tagged ERGIC markerDepositorInsertERGIC53 (Lman1 Mouse)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-IRES-Puro GFP-3xFLAG
Plasmid#201636PurposeOver-expression of 3xFLAG-tagged GFPDepositorInsertGFP
UseLentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
tetO-GFPnls-mPGK-rtTA-IRES-Puro
Plasmid#69545PurposeTet-On system expressing GFP tagged with 2x nuclear localization signals.DepositorInserteGFP
UseLentiviralTags2x nuclear localization sequence and T2A "sk…ExpressionMutationPromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-C-Myc-DDK-IRES-Puro HEXIM1
Plasmid#200726PurposeLentiviral expression of Flag-Tagged human HEXIM1DepositorInsertHEXIM1 (HEXIM1 Human)
UseLentiviralTagsMyc-DDK from backbone plasmidExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUbC-OsTIR1-myc-IRES-scFv-sfGFP
Plasmid#84563PurposeBicistronic vector for expression of O. sativa E3 ubiquitin ligase and single chain variable fragment of GCN4 antibody fused to superfolder GFPDepositorInsertTransport inhibitor response 1 (E3 ligase domain), IRES, single chain variable fragment-superfolder GFP
UseLentiviralTagsmyc tag (9x)ExpressionMutationPromoterAvailable sinceDec. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-DDdCas9VPH-T2A-GFP-IRES-Neo
Plasmid#102886PurposePiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
UseCRISPRTagsExpressionMammalianMutationD10A, H840APromoterCAGAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine
Plasmid#50454PurposeDouble floxed Gq-coupled hM3D-IRES-mCitrine under the control of human synapsin promoterDepositorUseAAVTagsHAExpressionMutationSee supplemental documents for DREADD mutationsPromoterhuman Synapsin 1Available sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-rtTA-IRES-GFP-WPRE-pA
Plasmid#183709PurposeAAV designed to express rtTA and eGFP from a CMV promoterDepositorInsertrtTA
UseAAVTagsExpressionMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2 LRP6-APEX2-Ires-eGFP-PAC
Plasmid#180142Purposeused to biotinylate targets that are recruited near the receptor during Wnt signaling at different time periodsDepositorInsertHuman LRP6 (LRP6 Human)
UseTagsExpressionMammalianMutationPromoterSP6Available sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti_sgRNA(MS2)_MCP-VP64-IRES-zsGreen1
Plasmid#138461Purposelenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-VP64DepositorInsertMCP-VP64-IRES-zsGreen1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1AAvailable sinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-RAB3C-IRES-Puromycin
Plasmid#134857PurposeExpresses EGFP-tagged synaptic vesicle markerDepositorInsertRas-related protein 3C
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_3C-Avi-His6_IRES-HA-BirA-ER
Plasmid#113898PurposeMammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of HA-tagged ER-resident E. coli biotin ligaseDepositorTypeEmpty backboneUseLentiviralTags3C-Avi-His6ExpressionMutationPromoterCMV-MIE-TetO2Available sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSH-EFIRES-P-AtAFB2-mCherry-weak NLS
Plasmid#129717PurposeAAVS1 safe harbor targeting vector expressing AtAFB2-mCherry-weak NLSDepositorInsertAFB2 (AFB2 Mustard Weed)
UseCRISPR and TALEN; Human safe harbor locus (a avs1…TagsmCherry-weak NLSExpressionMammalianMutationcodon optimizedPromoterEF1aAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-CMV-TetO2_3C-Avi-His6__IRES-EmGFP
Plasmid#113888PurposeMammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic EmGFPDepositorTypeEmpty backboneUseLentiviralTags3C-Avi-His6ExpressionMutationPromoterCMV-MIE-TetO2Available sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-TfR20-SNAP-IRES-Puro
Plasmid#171018PurposeLentiviral expression of a transferrin receptor-SNAP-tag fusion in mammalian cellsDepositorInsertTransferrin receptor (Human) - SNAP-tag (TFRC Human)
UseLentiviralTagsSNAPtag and TfRExpressionMammalianMutationPromoterEF1Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1328 LV EF1a-CD20 IRES-EGFP
Plasmid#201918Purpose2nd generation lentiviral transfer plasmid for engineering cells to express human CD20DepositorInsertCD20
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only