We narrowed to 9,921 results for: pET

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Ec dnaN-pET(11a)

Plasmid

#237238

Purpose

over-express E. coli dnaN (Pol III beta sliding clamp subunit)

Depositor

Michael O'Donnell

Insert

dnaN (beta) (dnaN E. coli)

Expression

Bacterial

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pET-His-E1GFP

Plasmid

#233885

Purpose

Bacterial Expression of recombinant His-tagged E1GFP

Depositor

Francesco Cardarelli

Publication

De Lorenzi et al Commun Biol. 2025 Feb 25;8(1):304. doi:

Insert

E1GFP

Tags

His

Expression

Bacterial

Mutation

T65S and T203Y compared to EGFP

Promoter

T7 promoter

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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YjdC-R82A_pET-14b

Plasmid

#233276

Purpose

Overexpress his-tag YjdC protein with a site directed mutagenesis, arginine at position 82 was substituted with alanine (R82A)

Depositor

Mohan Babu, Andrew Emili

Publication

Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

Insert

YjdC (yjdC )

Expression

Bacterial

Mutation

Arginine was replaced with Alanine at position 82…

Promoter

T7 promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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YjdC-R64A_pET-14b

Plasmid

#233263

Purpose

Overexpress his-tag YjdC protein with a site directed mutagenesis, arginine at position 64 was substituted with alanine (R64A)

Depositor

Mohan Babu, Andrew Emili

Publication

Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

Insert

YjdC (yjdC )

Expression

Bacterial

Mutation

Arginine was replaced with Alanine at position 64…

Promoter

T7 promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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HemH-R212A_pET-14b

Plasmid

#233262

Purpose

Overexpress his-tag HemH protein with a site directed mutagenesis, arginine at position 212 was substituted with alanine (R121A)

Depositor

Mohan Babu, Andrew Emili

Publication

Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

Insert

HemH (hemH )

Expression

Bacterial

Mutation

Arginine was replaced with Alanine at position 21…

Promoter

T7 promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET-MhCINS-6xHis

Plasmid

#194304

Purpose

Bacterial expression of MhCINS protein with a C terminal His-tag and transit peptide removed at N terminal

Depositor

Richard Saunders

Publication

Liu et al J Ecology 2024; 00, 1–20. doi: 10.1111/1

Insert

MhCINS

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pET-Cas9-FCPF

Plasmid

#220132

Purpose

Expresses Cas9-FCPF in E.coli

Depositor

Xinlai Cheng

Publication

Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

Insert

Cas9-FCPF (cas9 S. pyogenes)

Tags

His and NLS

Expression

Bacterial

Promoter

T7

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pET-dCas9-FCPF

Plasmid

#220133

Purpose

Expresses dCas9-FCPF in E.coli

Depositor

Xinlai Cheng

Publication

Altinbay et al Nucleic Acids Res. 2024 Sep 24:gkae798.

Insert

dCas9-FCPF (cas9 S. pyogenes, Synthetic)

Tags

6xHis

Expression

Bacterial

Mutation

FCPF sequence inserted on C-terminal

Promoter

T7

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET-NIR-FbLAG30

Plasmid

#220748

Purpose

NIR-FbLAG30 expression in bacteria.

Depositor

Vladislav Verkhusha

Publication

Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

Insert

NIR-FbLAG30

Use

Affinity Reagent/ Antibody

Expression

Bacterial

Mutation

For bacterial cytoplasmic expression, the pelB si…

Promoter

T7

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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SUMO-LPQTG-pET

Plasmid

#221851

Purpose

Expresses SUMO-2 with sortase motif and His tag

Depositor

Artur Krężel

Publication

Antonenko et al ACS Omega. 2024 Oct 30;9(45):45127-45137

Insert

SUMO-2 (SUMO2 Human)

Expression

Bacterial

Mutation

Gln88Leu, Gln89Pro

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET-His6-en1dFnCas9GFP

Plasmid

#201950

Purpose

Expression of en1dFnCas9-GFP in bacterial cells

Depositor

Debojyoti Chakraborty

Publication

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

Expression of en1dFnCas9-GFP in bacterial cells

Use

CRISPR

Tags

6x His, HA, NLS, and mEGFP

Expression

Bacterial

Mutation

E1369R on FnCas9

Promoter

T7

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pET-His6-en15dFnCas9GFP

Plasmid

#201951

Purpose

Expression of en15dFnCas9-GFP in bacterial cells

Depositor

Debojyoti Chakraborty

Publication

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

Expression of en15dFnCas9-GFP in bacterial cells

Use

CRISPR

Tags

6x His, HA, NLS, and mEGFP

Expression

Bacterial

Mutation

E1603H on FnCas9

Promoter

T7

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-syt1 5W

Plasmid

#213623

Purpose

Bacterial expression of SUMO-tagged rat syt1 5W

Depositor

Edwin Chapman

Publication

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

syt1 5W (Syt1 Rat)

Tags

SUMO

Expression

Bacterial

Mutation

M173W, F231W, F234W, C277A, V304W, Y364W, I367C

Available since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-syt1 s7+

Plasmid

#213624

Purpose

Bacterial expression of SUMO-tagged rat syt1 s7+

Depositor

Edwin Chapman

Publication

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

syt1 s7+ (Syt1 Rat)

Tags

SUMO

Expression

Bacterial

Mutation

L171K, H198K, K236R, C277A, N333R, Y364K, I 367C,…

Available since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-FL-syt1 5W

Plasmid

#213629

Purpose

Bacterial expression of SUMO-tagged rat FL-syt1 5W

Depositor

Edwin Chapman

Publication

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

FL-syt1 5W (Syt1 Rat)

Tags

SUMO

Expression

Bacterial

Mutation

M173W, F231W, F234W, C277A, V304W, Y364W, I367C

Available since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_04

Plasmid

#194665

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_04 in bacteria

Depositor

Kai Johnsson

Publication

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_04

Tags

His-tag and Strep-tag

Expression

Bacterial

Promoter

T7

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_07

Plasmid

#194668

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_07 in bacteria

Depositor

Kai Johnsson

Publication

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_07

Tags

His-tag and Strep-tag

Expression

Bacterial

Promoter

T7

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_08

Plasmid

#194669

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_08 in bacteria

Depositor

Kai Johnsson

Publication

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_08

Tags

His-tag and Strep-tag

Expression

Bacterial

Promoter

T7

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_10

Plasmid

#194671

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_10 in bacteria

Depositor

Kai Johnsson

Publication

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_10

Tags

His-tag and Strep-tag

Expression

Bacterial

Promoter

T7

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_11

Plasmid

#194672

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_11 in bacteria

Depositor

Kai Johnsson

Publication

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_11

Tags

His-tag and Strep-tag

Expression

Bacterial

Promoter

T7

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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