We narrowed to 15,323 results for: pET

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pET-28a mScarlet

Plasmid

#178543

Purpose

The mScarlet fluorescent protein with an N-terminal 6xHis-tag in pET28a.

Depositor

Siegfried Musser

Article

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

mScarlet

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pET29b-IPP1-His

Plasmid

#124137

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Inorganic pyrophosphatase (IPP1 Budding Yeast)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET-28a_6H-MMLV_RT_D524N-6H

Plasmid

#166945

Purpose

Bacterial expression of M-MLV reverse transcriptase

Depositor

Robert Tjian

Article

Graham et al PLoS One. 2021 Feb 3;16(2):e0246647. doi

Insert

gag-pol

Tags

6X His

Expression

Bacterial

Mutation

D524N, H8Y

Promoter

T7

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pET14b – E. coli Exonuclease I

Plasmid

#104552

Purpose

bacterial expression of Exonuclease I

Depositor

Charles Bell

Article

Korada et al Nucleic Acids Res. 2013 Jun;41(11):5887-

Insert

Exonuclease I (sbcB E.coli)

Tags

6X-His tag followed by thrombin site

Expression

Bacterial

Promoter

T7

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-pro-sumo1

Plasmid

#25101

Depositor

Guy Salvesen

Article

Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

Insert

pro-SUMO1 (SUMO1 Human)

Tags

His

Expression

Bacterial

Mutation

Changed Serine 2 to Alanine

Available Since

June 22, 2010

Availability

Academic Institutions and Nonprofits only
pET-pAG-Hia5

Plasmid

#174373

Purpose

Plasmid for IPTG-inducible expression and purification of protein A/G-Hia5-6xHis (pAG-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

Depositor

Aaron Straight

Article

Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

Insert

protein AG - hia5

Tags

6xHis and Protein A/G

Expression

Bacterial

Available Since

Sept. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-AviTag-SnoopLigase

Plasmid

#105626

Purpose

Plasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with AviTag for biotinylation

Depositor

Mark Howarth

Article

Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

Insert

AviTag-SnoopLigase

Expression

Bacterial

Promoter

T7

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pET21a-Streptavidin-Alive

Plasmid

#20860

Purpose

Alive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.

Depositor

Alice Ting

Article

Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

Insert

Streptavidin-Alive

Tags

His

Expression

Bacterial

Mutation

Wt gene

Available Since

April 21, 2009

Availability

Academic Institutions and Nonprofits only

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pET-45b(+)-Tn5

Plasmid

#112112

Purpose

Expresses hyperactive Tn5 transposase for next generation sequencing library preparation

Depositor

Frank Pugh

Article

Rossi et al Nat Commun. 2018 Jul 20;9(1):2842. doi:

Insert

Tn5

Tags

His6

Expression

Bacterial

Mutation

E54K E110K P242A L372P

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a SpyCatcher-SnoopCatcher

Plasmid

#72324

Purpose

Bacterial expression of SpyCatcher linked to SnoopCatcher.

Depositor

Mark Howarth

Article

Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

Insert

pET28a SpyCatcher-SnoopCatcher

Tags

N-terminal His6 tag

Expression

Bacterial

Mutation

35 amino acids at the N-term of SpyCatcher have b…

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pET21a-Streptavidin-Dead

Plasmid

#20859

Purpose

Dead subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Use in combination with Streptavidin-Alive (construct 20860).

Depositor

Alice Ting

Article

Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

Insert

Streptavidin-Dead

Expression

Bacterial

Mutation

Triple mutant of wt Streptavidin N23A,S27D,S45A

Available Since

April 21, 2009

Availability

Academic Institutions and Nonprofits only

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BARD1 26-327 pET28a

Plasmid

#12646

Depositor

Rachel Klevit

Article

Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

Insert

BRCA1 binding partner for a Ring domain E3 Ub-ligase (BARD1 Human)

Expression

Bacterial

Available Since

July 31, 2007

Availability

Academic Institutions and Nonprofits only

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pET28b-6His-AcpS

Plasmid

#145376

Purpose

Expression of E. coli AcpS

Depositor

Robert Tomko

Article

Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

Insert

AcpS (acpP E. coli)

Tags

6-His

Expression

Bacterial

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pET-CatC-HA-CfaNm

Plasmid

#241424

Purpose

Protein Transposon Cassette Containing CatC and CfaCNm

Depositor

Thomas Muir

Article

Hua et al Science. 2025 Apr 4;388(6742):68-74. doi

Insert

His5-Sumo-CatC-HA-CfaNm

Tags

His5

Expression

Bacterial

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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BirA in pET28a (w400-2)

Plasmid

#26624

Depositor

Eric Campeau

Insert

BirA

Tags

His

Expression

Bacterial

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only

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pET30a-AlkB-D135S

Plasmid

#79051

Purpose

Express mutant AlkB protein (D135S) in E. coli BL21(DE3)

Depositor

Tao Pan

Article

Zheng et al Nat Methods. 2015 Sep;12(9):835-7. doi:

Insert

AlkB (alkB E.coli)

Tags

His tag

Expression

Bacterial

Mutation

A truncated AlkB with deletion of the amino (N)-t…

Promoter

T7

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pET30a-His6-LOXCAT

Plasmid

#140084

Purpose

Fusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2.

Depositor

Vamsi Mootha

Article

Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

Insert

A fusion of lactate oxidase from A. viridans and catalase from E. coli

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pET-pro-siRNA-V2

Plasmid

#111086

Purpose

For production of recombinant siRNAs (pro-siRNAs) in E. coli.

Depositor

Linfeng HUANG

Article

Kaur et al Biotechnol Bioeng. 2018 Jun 5. doi: 10.1

Type

Empty backbone

Use

RNAi

Expression

Bacterial

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(0)

Plasmid

#199166

Purpose

used to express a neutral GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(0)

Tags

MGHHHHHHGGA

Mutation

E16R, I138R, D207R, N222K, L246R

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pET21-Streptavidin-K121R

Plasmid

#89880

Purpose

Streptavidin that has unimpaired biotin binding after dye labeling

Depositor

Mark Howarth

Article

Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-

Insert

Streptavidin-K121R-Glutamate_Tag

Tags

6 glutamate tag (C terminal on insert)

Expression

Bacterial

Mutation

K121R

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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