We narrowed to 2,117 results for: D10

1. pcDNA3-Lyso-TORCAR

   Plasmid

   #64929

   Purpose

   Lysosome-targeted FRET biosensor for monitoring mTORC1 kinase activity; targeted to cytosolic face of lysosomal membrane.

   Depositor

   Jin Zhang

   Article

   Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

   Insert

   LAMP1-TORCAR (LAMP1 Synthetic, Human)
   

   Use

   Tags

   Cerulean, Full-length LAMP1, and YPet

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF0725

   Plasmid

   #143071

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NFKB2 (NFKB2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-eNme2-T.1-ABE8e

   Plasmid

   #185668

   Purpose

   Expresses eNme2-T.1-ABE8e in mammalian cells

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

   Insert

   ecTadA(8e)-neNme2-T.2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2Cas9 D16A/E47K/V68M/T123A/D152G/E154K/T396A/H…

   Promoter

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGFP-mTet1s pc3901

   Plasmid

   #201700

   Purpose

   Expresses of GFP-tagged mTet1s in mammalian cells

   Depositor

   Cristina Cardoso

   Article

   Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

   Insert

   Tet1s (Tet1 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. CGBE1 (pRZ3885)

   Plasmid

   #140252

   Purpose

   CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   eUNG-BE4max(R33A)-ΔUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, D10A in Cas9

   Promoter

   CMV

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA GNSTM-3-RVG-10-Lamp2b-HA

   Plasmid

   #71294

   Purpose

   Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, RVG peptide, 10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.

   Depositor

   Joshua Leonard

   Article

   Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

   Insert

   Lamp2b (LAMP2 Human)
   

   Use

   Tags

   GNSTM glycosylation motif, HA, Lamp2 signal pepti…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-CR029

   Plasmid

   #234041

   Purpose

   expresses evoCDA1 BE4max base editor in a human T cell optimized lentiviral transfer plasmid

   Depositor

   Alexander Marson

   Article

   Schmidt et al Nature. 2024 Jan;625(7996):805-812. doi:

   Insert

   evoCDA1-Cas9(D10A)-P2A-blast
   

   Use

   Lentiviral

   Tags

   blast

   Expression

   Mutation

   Promoter

   Available since

   April 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-CR102

   Plasmid

   #234043

   Purpose

   expresses ABE8e(V106W) - SpG Cas9 base editor in a human T cell optimized lentiviral transfer plasmid

   Depositor

   Alexander Marson

   Article

   Schmidt et al Nature. 2024 Jan;625(7996):805-812. doi:

   Insert

   TadA8e(V106W)-SpG-Cas9(D10A)-P2A-blast
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. CGBE1-NG (pBM1246)

   Plasmid

   #140256

   Purpose

   CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   eUNG-BE4max(R33A)_NG-ΔUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

   Promoter

   CMV

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti PGK FKBP12(F36V)-OGT (Neo)

    Plasmid

    #154294

    Purpose

    Lentiviral vector encoding FKBP12(F36V)-2xHA-OGT (Neomycin resistant) off PGK promoter

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Lentiviral

    Tags

    2x HA and FKBP12(F36V)

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. TFORF0724

    Plasmid

    #143070

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NFKB2 (NFKB2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Str-KDEL_SBP-mCherry-LAMP1

    Plasmid

    #222318

    Purpose

    Synchronize the trafficking of LAMP1 from the ER.

    Depositor

    Franck Perez

    Article

    Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

    Insert

    Streptavidin-KDEL and LAMP1 fused to SBP-mCherry (LAMP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. Str-KDEL_SBP-EGFP-LAMP2

    Plasmid

    #222321

    Purpose

    Synchronize the trafficking of LAMP2 from the ER.

    Depositor

    Franck Perez

    Article

    Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

    Insert

    Streptavidin-KDEL and LAMP2 fused to SBP-EGFP (LAMP2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-dCas9-KRAB-PGK-HygR

    Plasmid

    #83890

    Purpose

    Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette.

    Depositor

    Charles Gersbach

    Article

    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

    Inserts

    humanized dead Cas9 KRAB
    aminoglycoside phosphotransferase from E. coli
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Expression

    Mutation

    D10A and H840A

    Promoter

    Human Ubiquitin C Promoter and mouse phosphoglyce…

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG R2pH-LAMP1-3xFLAG

    Plasmid

    #157940

    Purpose

    expresses ratiometric sensor of lysosomal lumenal pH

    Depositor

    Massimiliano Stagi

    Article

    Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

    Insert

    R2pH-LAMP1-3xFLAG (Lamp1 Mouse)
    

    Use

    Tags

    3XFLAG, mCherry, and pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    Chick beta actin pomoter

    Available since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDF-His-mTET1CD

    Plasmid

    #81053

    Purpose

    bacterial expression of the catalytic domain of murine TET1

    Depositor

    Primo Schaer

    Article

    Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

    Insert

    TET1 (Tet1 Mouse)
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    catalytic domain amino acid 1367-2057

    Promoter

    T7

    Available since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-eNme2-T.2-ABE8e

    Plasmid

    #185669

    Purpose

    Expresses eNme2-T.2-ABE8e in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    ecTadA(8e)-neNme2-T.2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2Cas9 D16A/E47K/R63K/V68M/A116T/T123A/D152N/E1…

    Promoter

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1/Myc-His beta4

    Plasmid

    #16039

    Depositor

    Filippo Giancotti

    Article

    Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-50

    Insert

    beta4 (ITGB4 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR-TRE3G-dCas9-10X GCN4-P2A-mCherry

    Plasmid

    #122132

    Purpose

    Express a dCas9, 10X GCN4, and mCherry cassette from the TRE3G promoter

    Depositor

    Stanley Qi

    Article

    Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

    Insert

    dCas9-10X GCN4-P2A-mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    dCas9 D10A & H840A

    Promoter

    TRE3G

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pF(UG) hSyn LAMP1-msGFP (JV012)

    Plasmid

    #179728

    Purpose

    Lentiviral expression of LAMP1-msGFP for use as a lysosomal marker

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    LAMP1 (Lamp1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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