We narrowed to 3,098 results for: actin

1. LifeAct-mGreenLantern

   Plasmid

   #164459

   Purpose

   Localization of mGreenLantern fluorescent protein to actin

   Depositor

   Gregory Petsko

   Article

   Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   mGreenLantern
   

   Use

   Tags

   LifeAct

   Expression

   Mammalian

   Mutation

   Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

   Promoter

   CMV

   Available since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTone-gata2aECE-Kaede

   Plasmid

   #132976

   Purpose

   gata2a endothelial enhancer (x6) and basal promoter driving kaede in pTol1 backbone

   Depositor

   Nathan Lawson

   Article

   Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

   Inserts

   Kaede
   gata2a endothelial enhancer (x6) and a carp b-actin basal promoter
   

   Use

   Unspecified; Transposon-mediated

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CAG-EGFP-P2A-FusionRed

   Plasmid

   #191108

   Purpose

   Mammalian cell line expression plasmids for production of EGFP and FusionRed proteins

   Depositor

   Kiryl Piatkevich

   Article

   Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

   Insert

   pAAV-CAG-EGFP-P2A-FusionRed
   

   Use

   AAV

   Tags

   FusionRed

   Expression

   Mammalian

   Mutation

   See depositor comments below

   Promoter

   chicken β-actin promoter

   Available since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pORANGE GFP-Actb KI #2

   Plasmid

   #139666

   Purpose

   Endogenous tagging of β-actin: N-terminal (amino acid position: before startcodon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-EB3mCherry_cmv-LifeActGFP

   Plasmid

   #133927

   Purpose

   Live dual labeling of the actin network and polymerizing microtubules for dynamic analysis of the cytoskeleton

   Depositor

   Frank Bradke, Sebastián Dupraz

   Article

   Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

   Insert

   EB3-mCherry and Lifeact-GFP (MAPRE3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBa.GFP-KIF1Bbeta tail 387-1770

   Plasmid

   #134620

   Purpose

   Mammalian expression of mouse KIF1Bbeta 387-1170

   Depositor

   Marvin Bentley

   Article

   Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

   Insert

   KIF1Bbeta (Kif1b Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   WT with deletion of the 386 N-terminal residues.

   Promoter

   Chicken Beta actin

   Available since

   April 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCardinal-Lifeact-7

   Plasmid

   #54663

   Purpose

   Localization: Actin, Excitation: 604, Emission: 659

   Depositor

   Michael Davidson

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Type

   Empty backbone

   Use

   Tags

   mCardinal-Lifeact

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. 2xFKBP-mCh-KDEL

   Plasmid

   #173010

   Purpose

   FKBP anchor for retaining DHFR-fused cargoes in the ER in the presence of zapalog. Encodes ER retrieval motif (KDEL) at C-terminus of mCherry. Contains IRES following ORF for cloning cargo.

   Depositor

   Matthew Kennedy

   Article

   Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

   Insert

   2xFKBP-mCh-KDEL
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken beta actin (CAG)

   Available since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5A 378-1027

   Plasmid

   #134611

   Purpose

   Mammalian expression of mouse KIF5A 378-1027

   Depositor

   Marvin Bentley

   Article

   Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

   Insert

   KIF5A (Kif5a Mouse)
   

   Use

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   WT with deletion of the 377 N-terminal residues.

   Promoter

   Chicken Beta actin

   Available since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAX-EGFP-FBP17

    Plasmid

    #178365

    Purpose

    Expresses FBP17 c-terminal labeled with EGFP in mammalian cells

    Depositor

    Erik Dent

    Article

    Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

    Insert

    Formin Binding Protein 17 (FNBP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken Beta Actin

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. DHFR-mNeon-GluA1

    Plasmid

    #173009

    Purpose

    For light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Intracellular trafficking can be better visualized with mNeon.

    Depositor

    Matthew Kennedy

    Article

    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    DHFR-mNeon-GluA1
    

    Use

    Tags

    DHFR and mNeon

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken beta actin (CAG)

    Available since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBa-flag-BicD2 594-FKBP

    Plasmid

    #64206

    Purpose

    Bicaudal D protein aa1-594 (excluding start Met) with N-terminal flag tag and C-terminal FKBP

    Depositor

    Marvin Bentley, Gary Banker

    Article

    Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

    Insert

    Bicaudal D protein (Bicd2 Mouse)
    

    Use

    Tags

    FKBP and flag

    Expression

    Mammalian

    Mutation

    no start methionine, aa1-594

    Promoter

    chicken Beta Actin

    Available since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBT268_(pCA-ATG-intron-tTA2-iiTRE-tdT3Mycii)

    Plasmid

    #36880

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    tTA2
    tdT-3Myc
    insulator
    

    Use

    Tags

    3 Myc tags

    Expression

    Mammalian

    Mutation

    insertion of a beta-globin intron with a loxP sit…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available since

    Aug. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENN.AAV.CB7.CI.CNDP2-flag.WPRE.rBG

    Plasmid

    #132685

    Purpose

    AAV plasmid encoding mouse CNDP2 with C-terminal flag tag

    Depositor

    Jonathan Long

    Article

    Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

    Insert

    Carnosine dipeptidase 2 (Cndp2 Mouse)
    

    Use

    AAV

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Chicken b-actin

    Available since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. 5HT6-YFP-ThymosinBeta4(WT)

    Plasmid

    #96806

    Purpose

    cilia-membrane-targeted G-actin-binding protein

    Depositor

    Takanari Inoue

    Article

    Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

    Insert

    5HT6-YFP-ThymosinBeta4 (Tmsb4x Mouse)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pME-Lifeact-EGFP-3xHA (JDW 1322)

    Plasmid

    #224494

    Purpose

    Gateway compatible middle entry clone containing Lifeact-EGFP (EGFP F-actin reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    Liefact-EGFP-3xHA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAGGS mSynCAM1

    Plasmid

    #115496

    Purpose

    expression of mouse full-length SynCAM 1, CAG promoter suited for neuronal expression

    Depositor

    Thomas Biederer

    Article

    Fogel et al J Neurosci. 2007 Nov 14;27(46):12516-30.

    Insert

    Cadm1 (Cadm1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    chicken beta-actin

    Available since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

18. FLAG-ARP8

    Plasmid

    #15361

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

    Insert

    actin-related protein 8 (ACTR8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTub-alpha1-EB3-NeonGreen-T2A-iLifeact-mCherry

    Plasmid

    #175261

    Purpose

    labelling of microtubule plus end and F-actin structures

    Depositor

    Frank Bradke, Sebastián Dupraz

    Article

    Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

    Insert

    EB3-NeonGreen-T2A-iLifeact-mCherry (MAPRE3 Human)
    

    Use

    Tags

    mCherry (on iLifeact) and mNeonGreen (on EB3)

    Expression

    Mammalian

    Mutation

    None (wt)

    Promoter

    pTub-alpha1

    Available since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCIG-VP16–RAR-alpha

    Plasmid

    #80434

    Purpose

    Expression of constitutively active human RAR-alpha in chick embryos

    Depositor

    Aixa Morales

    Article

    Martinez-Morales et al J Cell Biol. 2011 Aug 8;194(3):489-503.

    Insert

    Retinoic acid receptor alpha (RARA Human)
    

    Use

    Tags

    EGFP and VP16

    Expression

    Mammalian

    Mutation

    Promoter

    beta-actin promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only