We narrowed to 1,747 results for: lin tian

1. pSAH50 - [PVQp | gfp | tbb-2 UTR]

   Plasmid

   #200326

   Purpose

   gfp BioPart for PVQ neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.

   Depositor

   Christian Froekjaer-Jensen

   Article

   AlHarbi et al MicroPubl Biol. 2023 Jun 22;2023:10.1791

   Insert

   [PVQp | gfp | tbb-2 UTR]
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   WBGene00003755, nlp-17

   Available since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCK005 ubi:mCerulean_ubb-polyA

   Plasmid

   #195979

   Purpose

   Expression construct

   Depositor

   Alexa Burger, Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   ubi:mCerulean
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSEM264 - pDESTR4-R3 mosTI Pmlc-2_GFP target

   Plasmid

   #159842

   Purpose

   Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   pDESTR4-R3 mosTI Pmlc-2_GFP target
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSEM230 - pEXP[Pmlc-1 | ceGFP(2xNLS) | cbr-tbb-2 UTR]

   Plasmid

   #159794

   Purpose

   Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

   Insert

   pEXP[Pmlc-1 | ceGFP(2xNLS) | cbr-tbb-2 UTR]
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSEM323 - array co-injection fragment mosTI Pmlc-2_GFP

   Plasmid

   #159836

   Purpose

   Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   array co-injection fragment mosTI Pmlc-2_GFP
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP

   Plasmid

   #171090

   Purpose

   CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.

   Depositor

   Sebastian Lourido

   Article

   Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

   Inserts

   Sth1 dCas9
   Chloramphenicol acetyltransferase
   TagBFP
   

   Use

   CRISPR; Toxoplasma gondii expression

   Tags

   3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…

   Expression

   Mutation

   D9A, H599A

   Promoter

   TUB1 (Toxoplasma gondii), transcriptionally linke…

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTUB1-Spy.dCas9-CAT-U6-sgRNA(decoy)

   Plasmid

   #171089

   Purpose

   CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus pyogenes in Toxoplasma gondii. Suitable for generating stable cell lines.

   Depositor

   Sebastian Lourido

   Article

   Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

   Inserts

   Decoy sgRNA
   dCas9
   Chloramphenicol acetyltransferase
   

   Use

   CRISPR; Toxoplasma gondii expression

   Tags

   3X FLAG and Nuclear Localization Signal

   Expression

   Mutation

   D10A, H840A

   Promoter

   TUB1 (Toxoplasma gondii) and U6 (Toxoplasma gondi…

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. V2R untagged

   Plasmid

   #208911

   Purpose

   encodes the human vasopressin receptor 2, without any tags

   Depositor

   Christian Gruber

   Article

   Dekan et al Chem Sci. 2021 Feb 4;12(11):4057-4062. d

   Insert

   AVPR2 (AVPR2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. V1bR untagged

   Plasmid

   #208925

   Purpose

   encodes the human vasopressin receptor 1b, without any tags

   Depositor

   Christian Gruber

   Article

   Dekan et al Chem Sci. 2021 Feb 4;12(11):4057-4062. d

   Insert

   AVPR1B (AVPR1B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMS22H-IRE

    Plasmid

    #133905

    Purpose

    Positive control when used in combination with pAWH-IRP. Expression of MS2BS-IRE site hybrid. Homology regions for recombination with pAWH

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Insert

    IRE (Ftl1 Rat)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Pol III

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSEM414 - mosTI unc-119 Peft-3 - gfp(nls) - tbb-2

    Plasmid

    #182357

    Purpose

    Positive control for mosTI(unc-119). unc-119 rescue and ubiquitous nuclear GFP expression after mosTI insertion.

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Peft-3

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-EFS-LSL-nlsGFP-shRNA

    Plasmid

    #175584

    Purpose

    Conditional express shRNA with control of LoxP

    Depositor

    Christian Wolfrum

    Article

    Sun et al Nature. 2020 Nov;587(7832):98-102. doi:

    Type

    Empty backbone

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. mVenus-IQ6 R6A

    Plasmid

    #198202

    Purpose

    FRET acceptor: Mutated IQ6 domain of Moysin Va tagged with N-terminal mVenus (lower binding affinity)

    Depositor

    Christian Wahl-Schott

    Article

    Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

    Insert

    MYO5A IQ domain (Myo5a Mouse)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    R6A

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTSK561

    Plasmid

    #190816

    Purpose

    For C-terminal -Halo Tagging of a gene of interest with URA3 selection marker in yeast S. cerevisiae

    Depositor

    Tatiana Karpova, Gunjan Mehta

    Article

    Ball et al Nucleic Acids Res. 2016 Dec 1;44(21):e16

    Insert

    HaloTag
    

    Use

    Bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCas9-CAT

    Plasmid

    #80323

    Purpose

    Encodes Cas9 and chloramphenicol acetyltransferase (CAT)

    Depositor

    Sebastian Lourido

    Article

    Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

    Inserts

    Cas9
    Chloramphenicol acetyltransferase
    

    Use

    CRISPR

    Tags

    3X FLAG and NLS

    Expression

    Mutation

    Promoter

    TgTUB1

    Available since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. attB-mCh-Poly(A)

    Plasmid

    #65525

    Purpose

    Plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    attB-mCherry
    

    Use

    Mouse Targeting; Bxb1

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. attB-­GFP-­Poly(A)‐NeoR

    Plasmid

    #65526

    Purpose

    NeoR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    attB-GFP
    

    Use

    Mouse Targeting; Bxb1

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. N-TAP A2A in pcDNA3

    Plasmid

    #67850

    Purpose

    mammalian expression of A2A receptor with N terminal TAP tag

    Depositor

    Christian Gruber

    Article

    Bergmayr et al J Biol Chem. 2013 Oct 4;288(40):28831-44

    Insert

    adenosine A2a receptor (ADORA2A Human)
    

    Use

    Tags

    TAP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

19. attB-­mCh-­Poly(A)‐NeoR

    Plasmid

    #65528

    Purpose

    NeoR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    attB-mCherry
    

    Use

    Mouse Targeting; Bxb1

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. attB‐mCh­‐Poly(A)-­PuroR

    Plasmid

    #65529

    Purpose

    PuroR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    attB-mCherry
    

    Use

    Mouse Targeting; Bxb1

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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