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Plasmid#175758PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS8DepositorInsertLGALS8 (LGALS8 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pF3BGX-T2A-GAL4-DBD
Plasmid#138394PurposeRMCE vector for generating C-terminal T2A-GAL4-DBD fusion (split GAL4)DepositorInsertT2A-GAL4-DBD
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-i3 ONE-GO
Plasmid#189737PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-28a (+)-Gal4-vp16
Plasmid#171075PurposeExpresses Gal4-vp16 fusion protein in bacteriaDepositorInsertGal4 DBD (NEWENTRY Budding Yeast)
UseTagsHis and VP16 AD (413-490)ExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-GAL-Nat-ADE2
Plasmid#232103PurposeExpresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.DepositorInsertADE2 gRNA and repair template
UseCRISPRTagsExpressionYeastMutationRepair template introduces C at nt 466 of ADE2 to…PromoterGAL7Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
GAL-hAR-658-919
Plasmid#89082Purposemammalian expression of human androgen receptor ligand binding domain 658-919 amino acids linked to GAL4 DNA binding domainDepositorInserthAR-658-919 (AR Human)
UseTagsGAL4 DBDExpressionMammalianMutationaa 658-919PromoterAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-oA ONE-GO
Plasmid#189732PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralTagsExpressionMutationI250 (silent mutation in YFP)PromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-CMV-Galpha-13 ONE-GO
Plasmid#189740PurposeGPCR/G protein BRET biosensorDepositorInsertsUseLentiviralTagsExpressionMutationL342M (present in YFP but no functional consequen…PromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHACK-GAL4>QF2(newHA1)
Plasmid#104873PurposeHACK Donor plasmids for converting GAL4 lines to QF2 lines using HACK method. Updated version of Addgene#80275 for easier cloning of insertsDepositorInsertT2A-QF2
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
p415-GalL-Cas9-CYC1t
Plasmid#43804PurposeA human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the GalL promoterDepositorArticleInsertHuman Optimized S. pyogenes Cas9
UseCRISPRTagsExpressionYeastMutationPromoterGalLAvailable sinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only