We narrowed to 7,149 results for: CIT

1. pcDNA3-mNeptune2

   Plasmid

   #51309

   Purpose

   Expresses far-red fluorescent protein mNeptune2 in mammalian cells

   Depositor

   Michael Lin

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   mNeptune2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. FLAG-Skp2

   Plasmid

   #89142

   Purpose

   mammalian expression of human Skp2 with N-terminal FLAG tag

   Depositor

   Elizabeth Wilson

   Article

   Su et al Mol Cell Endocrinol. 2017 Jan 5;439:1-9.

   Insert

   Skp2 (SKP2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3.1-Flag-H2A K5-9R

   Plasmid

   #63562

   Purpose

   Expression of human H2A with K5R and K9R tagged with Flag

   Depositor

   Titia Sixma

   Article

   Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

   Insert

   H2A (H2AC20 Human)
   

   Use

   Tags

   Flag (MDYKDDDDKVPRIQ)

   Expression

   Mammalian

   Mutation

   Lysine 5 and Lysine 9 are now Arginine

   Promoter

   CMV

   Available since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

4. Cathepsin S S21C/C25A

   Plasmid

   #84695

   Purpose

   for expression of mutant Cathepsin S in Leishmania tarantolae

   Depositor

   Hans Brandstetter

   Article

   Freier et al Sci Rep. 2015 Aug 3;5:12707. doi: 10.103

   Insert

   cathepsin S (CTSS Human)
   

   Use

   Expression in leishmania tarantolae

   Tags

   his-tag

   Expression

   Mutation

   S21C, C25A

   Promoter

   unknown

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. CAG_mIGF1R_EGFP

   Plasmid

   #199629

   Purpose

   mutant FRET donor for IGF1R sensor

   Depositor

   Ryohei Yasuda

   Article

   Tu et al Sci Adv. 2023 Aug 2;9(31):eadg0666. doi:

   Insert

   mIGF1R (IGF1R Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Y1316F

   Promoter

   CAG

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV hSyn hPGK1-mRuby

   Plasmid

   #220911

   Purpose

   Neuronal specific expression of the fusion construct PGK1-mRuby

   Depositor

   Timothy Ryan

   Article

   Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

   Insert

   PGK1-mRuby (PGK1 Human)
   

   Use

   AAV

   Tags

   mRuby

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-miRFP680-NES-p2A-dTomato-2xrGBD

   Plasmid

   #222638

   Purpose

   Rho reporter

   Depositor

   Arnold Hayer

   Article

   Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

   Insert

   miRFP680-NES-p2A-dTomato-2xrGBD
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHH0103_SKAP2-1/1

   Plasmid

   #91447

   Purpose

   Protein expression and purification of human SH3 domain construct SKAP2-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   SKAP2-1/1 (SKAP2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. AM77_pU6.Sa-gRNA.CLYBL

   Plasmid

   #199237

   Purpose

   Expression construct encoding a S. aureus guide RNA targeting the human CLYBL safe harbor locus

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

   Insert

   S. aureus gRNA spacer
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   RNA polymerase III promoter for human U6 snRNA

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-CAG-sbGLuc-hGtARC2-EYFP

    Plasmid

    #114111

    Purpose

    fusion protein of Gaussia luciferase variant slow burn, Guillardia theta anion channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Prakash et al J Neurosci Res. 2018 Aug 28. doi: 10.100

    Insert

    Gaussia luciferase, slow burn; Guillardia theta anion channelrhodopsin-2, human codon optimized
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. plenti hSyn AldoA

    Plasmid

    #220916

    Purpose

    Neuronal specific expression of AldoA

    Depositor

    Timothy Ryan

    Article

    Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

    Insert

    AldoA (ALDOA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    June 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. CMV-GEX-GECO1

    Plasmid

    #32443

    Purpose

    Mammalian expression of green excitation ratiometric genetically encoded Ca2+ indicator for optical imaging

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    GEX-GECO1.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    GCaMP3 K69E/K119I/L173Q/T223S/F315L/F368L/L372Q/K…

    Promoter

    CMV

    Available since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pOPINK-OTUD1 (OTU+UIM, aa 287-481)

    Plasmid

    #61406

    Purpose

    Expresses human OTUD1 (OTU+UIM domains) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD1 (OTUD1 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-286.

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-cTNT-Cre-miR122 TS

    Plasmid

    #209784

    Purpose

    Expresses Cre by the specific cTNT promoter and incorporation of the miR122 target sequences (miR122TS) into the 3’ untranslated region (3’ UTR)

    Depositor

    Yuxuan Guo

    Article

    Yang et al Circulation. 2024 May 28;149(22):1778-17

    Insert

    miR122 ts
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    cTNT

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. xCas9(3.6)-ABE(7.10)

    Plasmid

    #108387

    Purpose

    Mammalian expression vector for xCas9(3.6)-ABE

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    xCas9(3.6)-ABE(7.10)
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

    Promoter

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. Venus-Noxa-pEGFP-C1

    Plasmid

    #166738

    Purpose

    Express Venus fused to the N-terminus of the Bcl-2 family protein, Noxa

    Depositor

    David Andrews

    Article

    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

    Insert

    Noxa (PMAIP1 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MoFGFR1c-ires neo

    Plasmid

    #86490

    Purpose

    mamalian cell expression of FGFR1c

    Depositor

    David Ornitz

    Article

    Ornitz et al J Biol Chem. 1996 Jun 21;271(25):15292-7

    Insert

    FgfrR1 IIIc 15D8 (Fgfr1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Moloney murine leukemia virus LTR

    Available since

    Feb. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. ELF4_pet28a

    Plasmid

    #131661

    Purpose

    Bacterial expression of ETS gene ELF4

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ELF4 (ELF4 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-Ef1a-fDIO-mRuby3-2A-dCre

    Plasmid

    #203845

    Purpose

    Flp-dependent enzymatically inactive Cre and mRuby3 expression

    Depositor

    Ivo Spiegel

    Article

    Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

    Insert

    dCre
    

    Use

    AAV

    Tags

    mRuby3-2A

    Expression

    Mutation

    Enzymatically inactive Cre

    Promoter

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-EF1a-Flex-DN-AMPK-K45R-T2A-mCherry

    Plasmid

    #83491

    Purpose

    cre-dependent DN-AMPK under EF1a promoter

    Depositor

    Dong Kong

    Article

    Kong et al Neuron. 2016 Jun 16. pii: S0896-6273(16)

    Insert

    DN-AMPK-2A-mCherry
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    K45R

    Promoter

    Available since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. ETV2_pet28a

    Plasmid

    #131665

    Purpose

    Bacterial expression of ETS gene ETV2

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ETV2 (ETV2 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. FL MALT1 WT in pET29b-Strep

    Plasmid

    #48968

    Purpose

    Expresses Full length wild type MALT1 in e.coli

    Depositor

    Guy Salvesen

    Article

    Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

    Insert

    MALT1 isoform A (MALT1 Human)
    

    Use

    Tags

    His and Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

23. pAGH10 - RlucFluc minus 1 HIV-1

    Plasmid

    #198224

    Purpose

    Fluorescence reporters for -1 ribosomal frameshifting in mammalian cells

    Depositor

    Isha Jain

    Article

    Baik et al Mol Cell. 2023 Mar 16;83(6):942-960.e9.

    Inserts

    Renilla luciferase
    Firefly luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV-dLbCpf1-BE-YE1

    Plasmid

    #154145

    Purpose

    Expresses dLbCpf1-BE-YE1(dLbCas12a-BE-YE1) in mammalian cells.

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Commun. 2020 Aug 13;11(1):4072. doi:

    Insert

    dLbCpf1-BE-YE1 (dCas12a-BE-YE1)
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    APOBEC1(W90Y+R126E), dLbCpf1(D832A+E925A+D1148A)

    Promoter

    CMV

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLV-RhoB Sensor (PKN-L59Q)-IRES-Blast

    Plasmid

    #222634

    Purpose

    Rho reporter, sensor dead

    Depositor

    Arnold Hayer

    Article

    Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

    Insert

    RhoB Sensor (PKN-L59Q)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    PKN L59Q

    Promoter

    EF1a

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAsCpf1(TATV)(BB) (pY221)

    Plasmid

    #89354

    Purpose

    Expresses humanized AsCpf1 TATV PAM variant and crRNA guide

    Depositor

    Feng Zhang

    Article

    Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

    Insert

    Cpf1 RVR variant
    

    Use

    CRISPR

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    S542R/K548V/N552R

    Promoter

    CBh

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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27. FEV_pet28a

    Plasmid

    #131668

    Purpose

    Bacterial expression of ETS gene FEV

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    FEV (FEV Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-TetON-Bmpr1A-ca

    Plasmid

    #217536

    Purpose

    AAV vector to drive the expression of Cre- and doxycycline-dependent expression of HA-epitope tagged constitutively-active BMPR1A receptor

    Depositor

    Peter Scheiffele

    Article

    Okur et al bioRxiv 2023

    Insert

    constitutively active BMP receptor 1a (BMPR1A Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Tet operon (TetO)

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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29. dxCas(3.6)-VPR

    Plasmid

    #108388

    Purpose

    Mammalian expression vector for xCas9 3.6 with VP64-p65-Rta (VPR) fused to its C-terminus

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    dxCas(3.6)-VPR
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

    Promoter

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-CAG-SuperNovaGreen-T2A-dTomato

    Plasmid

    #193798

    Purpose

    Expresses SuperNova Green in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomato

    Depositor

    Xiao Wang

    Article

    Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

    Insert

    SuperNova Green
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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