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pANTO3

Plasmid

#131107

Purpose

To generate a replicative plasmid carrying mycobacteriophage L5 integrase

Depositor

Riccardo Manganelli

Article

Boldrin et al Sci Rep. 2019 Apr 8;9(1):5783. doi: 10.1

Insert

mycobacteriophage L5 integrase

Expression

Bacterial

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pBbA20c-RFP

Plasmid

#126110

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

S23200

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pET21d-colEdes3/Imdes3

Plasmid

#107110

Purpose

plasmid for expression of computationally designed colicin endonuclease and immunity pair, des3

Depositor

Sarel Fleishman

Article

Netzer et al Nat Commun. 2018 Dec 11;9(1):5286. doi:

Insert

colEdes3/Imdes3

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pHL1801

Plasmid

#53039

Purpose

PLlacO-1:RBS(st7):gfp

Depositor

Han Lim

Article

Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

Insert

GFP

Use

Synthetic Biology

Tags

RBS(st7)

Expression

Bacterial

Promoter

pLlac0-1

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pHL1506

Plasmid

#53026

Purpose

PLlacO-1:RBS(st7):csrA

Depositor

Han Lim

Article

Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

Insert

csrA

Use

Synthetic Biology

Tags

RBS(st7)

Expression

Bacterial

Promoter

pLlac0-1

Available Since

May 13, 2014

Availability

Academic Institutions and Nonprofits only
UAS-fog

Plasmid

#41178

Depositor

Norbert Perrimon

Article

Hacker et al Genes Dev. 1998 Jan 15;12(2):274-84.

Insert

fog (fog Fly)

Expression

Insect

Promoter

Hsp70

Available Since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only
pHL1318

Plasmid

#37637

Depositor

Han Lim

Article

Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

Inserts

TetR
CsrA

Use

Synthetic Biology

Expression

Bacterial

Promoter

pConNoHind

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only
pWJPrP38

Plasmid

#22110

Depositor

Susan Lindquist

Article

Jackson et al Neuron. 2009 Aug 27. 63(4):438-50.

Insert

PrP genomic vector with cloning sites

Use

Construction of targeting vector

Mutation

2.2 Kb EcoR1 fragement isolated from pWJPrP34 and…

Available Since

Oct. 6, 2009

Availability

Academic Institutions and Nonprofits only
pc3XB-ZF11

Plasmid

#13110

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

ZF11

Expression

Mammalian

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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piHGpd/T22I

Plasmid

#1110

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Expression

Yeast

Mutation

HSP82 with threonine 22 changed to isoleucine; re…

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA5 HIS-Ub-K48R

Plasmid

#221368

Purpose

Expresses 6xHIS-tagged Ubiquitin containing K48R mutation in mammalian cells

Depositor

Aga Gambus

Article

Jones et al iScience. 2024 Jun 12;27(7):110260. doi:

Insert

Ubiquitin

Tags

6xHIS

Expression

Mammalian

Mutation

Changed Lysine 48 to Arginine

Promoter

CMV

Available Since

Aug. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-ZFN-AAVS1

Plasmid

#89707

Purpose

Mammalian expression plasmid for zinc finger nuclease targeting human AAVS1 locus

Depositor

Dmitriy Mazurov

Article

Zotova et al Viruses. 2017 Nov 2;9(11). pii: v9110325

Insert

ZFN-AAVS1

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-hMLH1dn-eGFP

Plasmid

#191104

Purpose

Lentiviral expression plasmid of hMLH1dn with P2A-eGFP reporter

Depositor

Hyongbum Kim

Article

Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

Insert

hMLH1dn

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCW-EGFP-Blast

Plasmid

#246561

Purpose

Lentiviral vector for inducible expression of EGFP (all-in-one Tet-On system)

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

GFP

Use

Lentiviral

Mutation

F64L, S65T (EGFP)

Promoter

tight TRE

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Fzd1/2/4/5/7/8 gRNA

Plasmid

#246550

Purpose

CRISPR vector co-expressing Cas9 and a mouse Fzd1/2/4/5/7/8 gRNA (conserved region)

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Fzd1/2/4/5/7/8 gRNA

Use

CRISPR

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Dvl1/3 gRNA

Plasmid

#246555

Purpose

CRISPR vector co-expressing Cas9 and a mouse Dvl1/3 gRNA (conserved region)

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Dvl1/3 gRNA

Use

CRISPR

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-HIF1 alpha

Plasmid

#21101

Depositor

Connie Cepko

Article

Chen et al Science. 2009 Jan 9. 323(5911):256-9.

Insert

HYPOXIA INDUCIBLE FACTOR 1ALPHA (HIF1A Human)

Expression

Mammalian

Available Since

May 5, 2009

Availability

Academic Institutions and Nonprofits only

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pCW-Flag-CtIP

Plasmid

#71108

Purpose

mammalian expession of Flag-CtlP

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

CtlP

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Please see Depositor Comments

Promoter

TRE promoter, Tet ON

Available Since

Nov. 11, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pET21a-GlyRS-His

Plasmid

#124110

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Glycyl-tRNA ligase (glyS, glyQ Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCW-Flag-CtIP-T847E

Plasmid

#71110

Purpose

mammalian expession of Flag-CtlP T847E mutation

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

CtlP

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

T847E

Promoter

TRE promoter, Tet ON

Available Since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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