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pcDNA3.2 mir-1-1 reporter mir16-1 wt

Plasmid

#46683

Purpose

Expresses wild-type miR-16-1 in mammalian cells

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir16-1 wt (MIR16-1 Synthetic, Human)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-NCre-MBP6-WPRE

Plasmid

#193916

Purpose

Expresses one of the components of Cre-DOR_N6C1 (RFP-dependent Cre)

Depositor

Tatsushi Onaka

Article

Inutsuka et al Commun Biol. 2022 Sep 16;5(1):979. doi:

Insert

NCre-MBP6

Use

AAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMKO.1-puro/hFOXH1 shRNA1

Plasmid

#59303

Purpose

Knockdown of human FOXH1

Depositor

Shinya Yamanaka

Article

Takahashi et al Nat Commun. 2014 Apr 24;5:3678. doi: 10.

Insert

hFOXH1 shRNA (FOXH1 Human)

Use

RNAi and Retroviral

Promoter

hU6

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
8xCTS1_deltaC55A-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

Plasmid

#124539

Purpose

Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

Depositor

Tudor Fulga

Article

Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

Insert

deltaC55A-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro

Expression

Mammalian

Mutation

d11-41 C55A, d11-41

Promoter

Minimal ADE

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pONL-C1_Rab11a

Plasmid

#65707

Purpose

Expresses ONL-Rab11a in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Rab11a (Rab11a Mouse)

Use

Luciferase

Tags

ONL

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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FB029

Plasmid

#119713

Purpose

Promoter paf from Penicillium chrysogenum domesticated into pUPD2 with GGAG/AATG barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation.

Depositor

Jose Marcos

Article

Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

Insert

Promoter paf

Use

Synthetic Biology; Domestication of dna parts for…

Promoter

Promoter paf

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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FB030

Plasmid

#119714

Purpose

Terminator paf from P. chrysogenum domesticated into pUPD2 with GCTT/CGCT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation

Depositor

Jose Marcos

Article

Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

Insert

Terminator paf

Use

Synthetic Biology; Domestication of dna parts for…

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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ADCK3 gRNA (BRDN0001147832)

Plasmid

#77321

Purpose

3rd generation lentiviral gRNA plasmid targeting human ADCK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ADCK3

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ADCK3 gRNA (BRDN0001146491)

Plasmid

#77323

Purpose

3rd generation lentiviral gRNA plasmid targeting human ADCK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ADCK3

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pSpCAS9n(BB)-2A-puro DNM1, A

Plasmid

#107795

Purpose

gRNA vector for knock-in gene editing at human DNM1 locus

Depositor

Sandra Schmid

Article

Srinivasan et al PLoS Biol. 2018 Apr 18;16(4):e2005377. d

Insert

gRNA targeting human DNM1

Use

CRISPR

Expression

Mammalian

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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