Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 5,174 results for: Apoe

Showing: 2581 - 2610 of 5174 results

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pKLV2-U6gRNA5(hCASP8_1k)-PGKpuro2ABFP-W

Plasmid

#208422

Purpose

Lentiviral gRNA plasmid targeting human CASP8 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

CASP8 (CASP8 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hCASP8_2b)-PGKpuro2ABFP-W

Plasmid

#208423

Purpose

Lentiviral gRNA plasmid targeting human CASP8 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

CASP8 (CASP8 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hTNF_2b)-PGKpuro2ABFP-W

Plasmid

#208426

Purpose

Lentiviral gRNA plasmid targeting human TNF gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

TNF (TNF Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hMAP3K7_1k)-PGKpuro2ABFP-W

Plasmid

#208412

Purpose

Lentiviral gRNA plasmid targeting human MAP3K7 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

MAP3K7 (MAP3K7 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hMAP3K7_2m)-PGKpuro2ABFP-W

Plasmid

#208413

Purpose

Lentiviral gRNA plasmid targeting human MAP3K7 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

MAP3K7 (MAP3K7 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pLX304-BCL-XL-F97W

Plasmid

#203572

Purpose

Expresses V5-tagged BCL-XL with partial drug resistance to A-1331852 in mammalian cells.

Depositor

Abhishek Chakraborty

Article

Grubb et al Clin Cancer Res. 2022 Nov 1;28(21):4689-

Insert

BCL2L1 (BCL2L1 Human)

Use

Lentiviral

Tags

V5-tagged

Expression

Mammalian

Mutation

Changed Phenylalanine 97 to Tryptophan for partia…

Promoter

CMV

Available Since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pLX304-BCL-XL-499R

Plasmid

#203574

Purpose

Expresses V5-tagged BCL-XL with resistance to shRNA (TRCN0000033499) in mammalian cells.

Depositor

Abhishek Chakraborty

Article

Grubb et al Clin Cancer Res. 2022 Nov 1;28(21):4689-

Insert

BCL2L1 (BCL2L1 Human)

Use

Lentiviral

Tags

V5- tagged

Expression

Mammalian

Mutation

silent point mutations introduced to make it resi…

Promoter

CMV

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFR*-CUbo(K63R)-His6

Plasmid

#212820

Purpose

Constitutive or doxycycline-inducible expression of EGFR*-CUbo(K63R)-His6 in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

EGFR* (EGFR Budding Yeast, Human)

Tags

CUbo(K63R)-6xHis

Expression

Mammalian

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFR*-CUbo(K48R)-GFP

Plasmid

#212816

Purpose

Constitutive or doxycycline-inducible expression of EGFR*-CUbo(K48R)-GFP in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

EGFR* (EGFR Budding Yeast, Human)

Tags

CUbo(K48R)-GFP

Expression

Mammalian

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-FRT-TO-EGFR*-CUbo(K63R)-GFP

Plasmid

#212817

Purpose

Constitutive or doxycycline-inducible expression of EGFR*-CUbo(K63R)-GFP in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

EGFR* (EGFR Budding Yeast, Human)

Tags

CUbo(K63R)-GFP

Expression

Mammalian

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFR*-CUbo(K48R K63R)-GFP

Plasmid

#212818

Purpose

Constitutive or doxycycline-inducible expression of EGFR*-CUbo(K48R K63R)-GFP in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

EGFR* (EGFR Budding Yeast, Human)

Tags

CUbo(K48R/K63R)-GFP

Expression

Mammalian

Available Since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pT2/shCol1a1/Scarlet_Seq1.3

Plasmid

#206137

Purpose

Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

Insert

COL1A1 (Col1a1 Mouse)

Expression

Mammalian

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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Tet-Inducible Mouse C325A Caspase-9 Mutant

Plasmid

#194500

Purpose

Inducible Expression of Mouse C325A Caspase-9 Mutant

Depositor

Hannah Rabinowich

Article

Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649

Insert

Mouse Caspase-9 C325A Mutant (Casp9 Mouse)

Tags

None

Expression

Mammalian

Mutation

Changed Cysteine 325 to Alanine to eliminate prot…

Promoter

CMV

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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Tet-Inducible Human Caspase-9 Alpha C287A Mutant

Plasmid

#194709

Purpose

Inducible Expression of Human Caspase-9 Alpha C287A Mutant

Depositor

Hannah Rabinowich

Article

Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649

Insert

Human Caspase-9 C287A Mutant (CASP9 Human)

Tags

None

Expression

Mammalian

Mutation

Changed Cysteine 287 to Alanine to eliminate prot…

Promoter

CMV

Available Since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Tet-inducible 3xFlag Mouse C325A Caspase-9 Mutant

Plasmid

#194501

Purpose

Inducible Expression of N-Terminal 3X FLAG Mouse Caspase-9 C325A Mutant

Depositor

Hannah Rabinowich

Article

Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649

Insert

Caspase-9 C325A Mutant (Casp9 Mouse)

Tags

3X Flag

Expression

Mammalian

Mutation

Changed Cysteine 325 to Alanine to eliminate prot…

Promoter

CMV

Available Since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Venus-Puma-4E-pEGFP-C1

Plasmid

#166746

Purpose

Express Venus fused to the N-terminus of the Bcl-2 family protein, Puma with mutation in BH3 domain to disrupt binding anti-apoptotic proteins

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

Puma-4E (BBC3 Human)

Tags

Venus

Expression

Mammalian

Mutation

4 hydrophobic residues in the BH3 region mutated …

Promoter

CMV

Available Since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
P2_DD-HNF4A8_C106R

Plasmid

#31096

Depositor

Gerhart Ryffel

Article

Senkel et al BMC Res Notes. 2009 Oct 17;2:210. doi: 1

Insert

HNF4A (HNF4A Human)

Use

Flp/frt

Tags

FKBP L106P and myc

Expression

Mammalian

Mutation

splice variant 8 derived from promoter P2 with zi…

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-PFKFB3

Plasmid

#23668

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PFKFB3 (PFKFB3 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-PDK1

Plasmid

#23804

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PDK1 (PDK1 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-PDK3

Plasmid

#23556

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PDK3 (PDK3 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-HK2

Plasmid

#23854

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

HK2 (HK2 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PFKP

Plasmid

#23869

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PFKP (PFKP Human)

Use

Gateway donor vector

Mutation

1MM

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-PDPK1

Plasmid

#23801

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PDPK1 (PDPK1 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PKM2

Plasmid

#23757

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PKM2 (PKM Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-STK38

Plasmid

#23811

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

STK38 (STK38 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PCK2

Plasmid

#23715

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PCK2 (PCK2 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pUCmini-iCAP-AAV.CAP-B10

Plasmid

#175004

Purpose

non-standard AAV2 rep-AAV.CAP-B10 cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation system

Depositor

Viviana Gradinaru

Article

Goertsen et al Nat Neurosci. 2022 Jan;25(1):106-115. do

Insert

Synthetic construct isolate AAV.CAP-B10 VP1 gene

Use

AAV

Expression

Mammalian

Mutation

7 amino acid substitution between VP1 452 and VP1…

Promoter

p41

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-PFKM

Plasmid

#23728

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PFKM (PFKM Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-PFKL

Plasmid

#23729

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PFKL (PFKL Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-PRKCQ

Plasmid

#23424

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKCQ (PRKCQ Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 2581 - 2610 of 5174 results

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