We narrowed to 10,315 results for: 158

1. pDF0352 HvsCas7-11 with C-term Nes mammalian expression

   Plasmid

   #172516

   Purpose

   Encodes human codon optimized HvsCas7-11 protein with C-terminal NES tag for expression in mammalian cells

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   C-term Nes HvsCas7-11 mammalian expression
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDF0118 DisCas7-11 bacterial expression with Twinstrep-SUMO-Codon

   Plasmid

   #172503

   Purpose

   Encodes the DisCas7-11 protein only for bacterial recombinant protein expression for purification

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   DisCas7-11 bacterial expression with Twinstrep-SUMO-Codon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFastBac-His-MBP-SCov2-nsp12

   Plasmid

   #154759

   Purpose

   Expression of SARS-CoV-2 nsp12

   Depositor

   Patrick Cramer

   Article

   Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

   Insert

   SARS-CoV-2 nsp12 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Polyhedron

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pscALPS- HIV2-ROD vpx

   Plasmid

   #115816

   Purpose

   Encodes codon optimized 3xFLAG-HIV2ROD Vpx (M15390) and puromycin resistance protein

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   HIV2ROD Vpx (M15390)
   

   Use

   Lentiviral

   Tags

   3x-FLAG

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAPM-D4 miR30-TRIM28 ts1

   Plasmid

   #115862

   Purpose

   TRIM28 knockdown

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   TRIM28 shRNA (TRIM28 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SpCas9-CBE6a-V106W

   Plasmid

   #215824

   Purpose

   Express CBE6a-V106W (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6a V106W-SpCas9-2xUGI (cas9 )
   

   Use

   In vitro transcription template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-TIWB-yellow pre-eGRASP(p30)

   Plasmid

   #111588

   Purpose

   An AAV vector that expresses yellow pre-eGRASP(p30) under the tetO promoter.

   Depositor

   Bong-Kiun Kaang

   Article

   Choi et al Science. 2018 Apr 27;360(6387):430-435.

   Insert

   yellow pre-eGRASP(p30)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   tetO

   Available since

   June 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pU6-sp-sgRNA-mDNMT1_nick-lenti

   Plasmid

   #135959

   Purpose

   S. pyogenes nicking sgRNA for mouse DNMT1

   Depositor

   David Liu

   Article

   Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

   Insert

   mDNMT1_nick
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   See manuscript

   Promoter

   U6

   Available since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-TIWB-yellow pre-eGRASP(p32)

   Plasmid

   #111582

   Purpose

   An AAV vector that expresses yellow pre-eGRASP(p32) under the tetO promoter.

   Depositor

   Bong-Kiun Kaang

   Article

   Choi et al Science. 2018 Apr 27;360(6387):430-435.

   Insert

   yellow pre-eGRASP(p32)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   tetO

   Available since

   June 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. G1333 DddAtox-C–dSpCas9

    Plasmid

    #157834

    Purpose

    expresses split DddAtox-Cas9 construct in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    bpNLS–G1333 DddAtox-C–dSpCas9–UGI–UGI–bpNLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1-SARS-CoV-2-E

    Plasmid

    #215814

    Purpose

    Expresses SARS-CoV-2 Envelope protein in mammalian cells

    Depositor

    Thomas Gallagher

    Article

    Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

    Insert

    SARS-CoV-2 Envelope (E )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-SARS-CoV-2-M

    Plasmid

    #215813

    Purpose

    Expresses SARS-CoV-2 Membrane protein in mammalian cells

    Depositor

    Thomas Gallagher

    Article

    Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

    Insert

    SARS-CoV-2 Membrane (M )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1-SARS-CoV-2-S (D614G)

    Plasmid

    #215838

    Purpose

    Expresses SARS-CoV-2 Spike protein in mammalian cells

    Depositor

    Thomas Gallagher

    Article

    Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

    Insert

    SARS-CoV-2 Spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMT2-HA-MKK3

    Plasmid

    #21580

    Depositor

    John Kyriakis

    Article

    Molnar et al J Biol Chem. 1997 May 16. 272(20):13229-

    Insert

    mitogen-activated protein kinase kinase 3 (MAP2K3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 24, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pscALPS-HIV-1 Group P-vpr

    Plasmid

    #115837

    Purpose

    Encodes codon optimized 3xFLAG-HIV1 Group P Vpr (HQ179987) and puromycin resistance protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    HIV1 Group P Vpr (HQ179987)
    

    Use

    Lentiviral

    Tags

    3x-FLAG

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDF0191 CjcCas7-11 bacterial expression with Twinstrep-SUMO-Codon

    Plasmid

    #172505

    Purpose

    Encodes the CjcCas7-11 protein only for bacterial recombinant protein expression for purification

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    CjcCas7-11 bacterial expression with Twinstrep-SUMO-Codon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAPM-D4 miR30-SUV39h1 ts2

    Plasmid

    #115881

    Purpose

    SUV39h1 knockdown

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SUV39h1 shRNA (SUV39H1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAPM-D4 miR30-SUV39h1 ts1

    Plasmid

    #115880

    Purpose

    SUV39h1 knockdown

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SUV39h1 shRNA (SUV39H1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCHA1.1-MFSD12-V5

    Plasmid

    #149508

    Purpose

    Stable expression of human MFSD12 with recoded nucleotide sequence.

    Depositor

    David Sabatini

    Article

    Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

    Insert

    MFSD12 (MFSD12 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Codon Optimized Sequence

    Promoter

    Ubc

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDF0336 pAAV EFS-HvsCas7-11 for dual vector

    Plasmid

    #172515

    Purpose

    Encodes HvsCas7-11 mammalian expression casette in AAV backbone

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    pAAV EFS-HvsCas7-11 for dual vector
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge