We narrowed to 2,240 results for: ARP

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pVD1 Multiplexing Edit E3-E4-En-1 (GB2244)

Plasmid

#160566

Purpose

GBoligomers for the position [3_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

Insert

Multiplexing Edit (E3-E4-En-1)

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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EFS-FLAG-SpCas9-6xNLS(c-Myc)-P2A-Puro (pRG214)

Plasmid

#221385

Purpose

Lentiviral expression of SpCas9-6xNLS(c-Myc) with puromycin resistance gene

Depositor

Junwei Shi

Article

Ngiow et al Cell. 2024 Aug 8;187(16):4336-4354.e19.

Insert

SpCas9

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

June 11, 2024

Availability

Academic Institutions and Nonprofits only

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pQCXIP mScarlet-ER [TM(SAC1)]

Plasmid

#186572

Purpose

Expression of the Endoplasmic Reticulum marker mScarlet-ER [TM(SAC1)] in mammalian cells

Depositor

Catherine Tomasetto

Article

Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

SAC1 (SACM1L Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

C-terminal transmembrane region of the protein: 5…

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAcBac1-Ma-sfGFP 150TAG

Plasmid

#197568

Purpose

Expression of sfGFP-150TAG with Methanomethylophilus alvus (Ma) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

Depositor

Ryan Mehl

Article

Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

Inserts

sfGFP 150TAG
M. alvus Pyl-tRNA (6) (4x copies)

Tags

V5-His6

Expression

Mammalian

Mutation

N150TAG

Promoter

CMV and U6/H1

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5 FLAG 17xS/T->A, catalytic dead lipin 1

Plasmid

#32008

Depositor

David Sabatini

Article

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

Lipin1 (Lpin1 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

D712E, D714E AND S106A, S150A, (S281A, T282A), S2…

Promoter

CMV, SP6

Available Since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only
pDGB3 alpha2 P35S:dCas9:VPR:Tnos (GB1826)

Plasmid

#160623

Purpose

TU for the constitutive expression of dCas9 fused to VPR (VP64-p65-Rta) activation domains

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

P35S:dCas9:VPR-Tnos

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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mApple-Actin-C-18

Plasmid

#54863

Purpose

Localization: Actin, Excitation: 568, Emission: 592

Depositor

Michael Davidson

Article

Thievessen et al J Cell Biol. 2013 Jul 8;202(1):163-77. d

Insert

Actin (ACTB Human)

Tags

mApple

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only
pRK5-LYCHOS (GPR155) WT-HA

Plasmid

#199660

Purpose

Transient expression of LYCHOS (GPR155) WT

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

GPR155 (GPR155 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pQCXIP mScarlet-MOSPD2 W201E

Plasmid

#186477

Purpose

Expression of human MOSPD2 (mutant W201E, unable to bind lipid droplets) fused to mScarlet in mammalian cells

Depositor

Catherine Tomasetto

Article

Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Use

Retroviral

Tags

mScarlet

Expression

Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pVD1 M1_1-pTRNA-scf 2.1 (GB2603)

Plasmid

#160568

Purpose

tRNA and scaffold 2.1 scRNA aptamer Ms2 for the assembly of GBoligomers for a single position of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

M1_1-pTRNA-scf 2.1

Use

CRISPR

Mutation

BsaI and BsmBI sites removed

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pET51b-Strep-hAGT-His

Plasmid

#167276

Purpose

Overexpression of the human o_-alkylguanine-dna alkyltransferase in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

hAGT (AGT Human)

Tags

His x10 and Strep-tag

Expression

Bacterial

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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R-Q111R-N122D

Plasmid

#167178

Purpose

Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-R variant with reverted Q111R and N122D mutations in Sf9 cells

Depositor

Susanne Dobler

Article

Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

Inserts

ATP1A1-R-Q111R-N122D
ATP1B1

Expression

Bacterial and Insect

Mutation

Changed R at 111 to Q and changed D at 122 to N

Promoter

P10 and PH

Available Since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pRS416 ATG5-E141D-HA-ATG12-ATG16

Plasmid

#166851

Purpose

Expresses mutant E141D ATG5, HA-ATG12 ATG16 in yeast.

Depositor

Daniel Klionsky

Article

Kim et al Elife. 2016 Jan 26;5. pii: e12245. doi:

Insert

ATG5 ATG12 ATG16 (ATG5, ATG12, ATG16 Budding Yeast)

Tags

HA-ATG12

Expression

Yeast

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pVD1 Multiplexing Edit E4 (GB2241)

Plasmid

#160563

Purpose

tRNA and scaffold for the assembly of GBoligomers for position [4-5] of a polycistronic tRNA-gRNA.

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

Insert

Multiplexing Edit (E4)

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti CGIP + M-AAT target

Plasmid

#86008

Purpose

lenti reporter plasmid with M-AAT-specific gRNA target-sequence, to be used with tGFP #26864

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Inserts

CAG promoter
M-AAT target

Use

Lentiviral

Mutation

V30M

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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modRNA1 2x k-turn CLTX CD3z CAR

Plasmid

#213985

Purpose

Chlorotoxin (CLTX)-CAR huCD3z gene (first generation without any co-stimulatory domain) in modRNA cap1 vector

Depositor

Xiaoping Bao

Article

Chang et al Nat Biomed Eng. 2026 Apr 24:10.1038/s415

Insert

CLTC CAR

Use

Synthetic Biology

Expression

Mammalian

Available Since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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pJL388

Plasmid

#243717

Purpose

Stably expresses a Myc-tagged AKAP1 with a GDDG mutation and gRNA-resistant silent mutations

Depositor

Jonathan Weissman

Article

Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.

Insert

AKAP1 (AKAP1 Human)

Use

Lentiviral

Tags

Myc

Mutation

GXXG motif (GKQG 624-627) mutated to GDDG, and gR…

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pJH061

Plasmid

#243722

Purpose

Expresses a chimeric form of ALDH18A1, where the region between residue 97 and 351 is replaced by a 250-residue XTEN linker

Depositor

Jonathan Weissman

Article

Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.

Insert

ALDH18A1(1-97aa)-XTEN250-ALDH18A1(351aa-stop) (ALDH18A1 Human)

Use

Lentiviral

Mutation

The region between residue 97 and 351 of ALDH18A1…

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pET28 His-SUMO-CHMP2B

Plasmid

#232018

Purpose

Bacterial expression of His and SUMO tagged CHMP2B

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

6x His, SUMO

Expression

Bacterial

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pET-22b(+)_PelB-BN002P-M-FLAG-His

Plasmid

#218026

Purpose

RESOLUTE SLC binder expression plasmid

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

Insert

BN002P-M

Tags

1xFLAG 10xHis and PelB

Expression

Bacterial

Available Since

May 9, 2024

Availability

Academic Institutions and Nonprofits only

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