We narrowed to 6,346 results for: sas;

1. pSLQ2833 pPB: CAG-PYL1-VPR-IRES-Zeo-WPRE-SV40PA PGK-ABI-tagBFP-SadCas9

   Plasmid

   #84243

   Purpose

   Expresses ABA-inducible VPR-Sa dCas9

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   ABI-tagBFP-Sa dCas9
   PYL1-VPR
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   ABI-tagBFP-Nucleoplasmin NLS, IRES-Zeocin, PYL1, …

   Expression

   Mammalian

   Mutation

   D10A, N580A and Synonymous mutation in PYL1 to re…

   Promoter

   CAG and PGK

   Available since

   Nov. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOPINB-hRNF216(457-784)

   Plasmid

   #171922

   Purpose

   Expression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells. Catalytic helix-RBR-helix construct suitable for activity assays.

   Depositor

   Bernhard Lechtenberg

   Article

   Cotton et al Mol Cell. 2022 Feb 3;82(3):598-615.e8. d

   Insert

   E3 ubiquitin-protein ligase RNF216 (RNF216 Human)
   

   Use

   Tags

   3C protease cleavage site and 6x-His tag

   Expression

   Bacterial

   Mutation

   codon optimised for expression in E. coli

   Promoter

   T7

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. MmEos3.2

   Plasmid

   #203754

   Purpose

   Encodes the membrane anchor of Src including the first 15 resideues of the N terminus, with 1 myristoylation and short polybasic sequence, with mEos3.2 fused to the C terminus. Used as a fluorescent plasma membrane probe.

   Depositor

   Sarah Veatch

   Article

   Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

   Insert

   Src15 n-term (SRC Human)
   

   Use

   Tags

   mEos3.2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDFDuet-nsp7-nsp8

   Plasmid

   #159092

   Purpose

   Coexpression construct of Nsp7 with an N-terminal His-tag and Nsp8

   Depositor

   Seth Darst

   Article

   Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

   Inserts

   NSP7
   NSP8
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHiUGE TurboID-HA donor (all-in-one) ORF1

   Plasmid

   #200384

   Purpose

   Tagging endogenously expressed proteins with TurboID-HA, C-terminal

   Depositor

   Scott Soderling

   Article

   Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

   Insert

   TurboID (ref: PMID: 30125270, Addgene plasmid # 107169)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHiUGE smFP-V5 donor (all-in-one) ORF0

   Plasmid

   #200386

   Purpose

   Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

   Depositor

   Scott Soderling

   Article

   Gao et al Nat Commun. 2024 Aug 9;15(1):6801. doi:

   Insert

   spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-sACE2(WT)-8his

   Plasmid

   #149268

   Purpose

   Mammalian expression plasmid for his-tagged sACE2

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   Angiotensin-converting enzyme 2 (ACE2 Human)
   

   Use

   Tags

   Short GSG linker and 8his tag

   Expression

   Mammalian

   Mutation

   Extracellular, soluble protease domain (a.a. M1-D…

   Promoter

   CMV

   Available since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. LbCas12a-2C-NLS in pCSDest

   Plasmid

   #126638

   Purpose

   Expresses LbCas12a-2C-NLS in mammalian cell

   Depositor

   Scot Wolfe

   Article

   Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

   Insert

   LbCas12a-2C-NLS
   

   Use

   CRISPR

   Tags

   3xHuman influenza hemagglutinin (HA)-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94 promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-tetON-NOTUM-EF1a-TagRFP-2A-tet3G

   Plasmid

   #204188

   Purpose

   Doxycycline inducible lentiviral vector for human NOTUM overexpression

   Depositor

   Emma Rawlins

   Article

   Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

   Insert

   NOTUM (NOTUM Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetON

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDF1-PTPRD-G446E

    Plasmid

    #25643

    Depositor

    Todd Waldman

    Article

    Solomon et al Cancer Res. 2008 Dec 15. 68(24):10300-6.

    Insert

    Protein tyrosine phosphatase receptor-type delta (PTPRD Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    tumor-derived mutation created by site-directed m…

    Promoter

    Available since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pCEP4-myc-HLA-A3

    Plasmid

    #135505

    Purpose

    Mammalian expression of myc-tagged HLA-A*03:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*03:01 (HLA-A Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pegCTNNB1 S45del

    Plasmid

    #169844

    Purpose

    pegRNA used for installation (via TCC deletion) of the oncogenic S45del in Ctnnb1 in mouse liver.

    Depositor

    Wen Xue

    Article

    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

    Insert

    pegCTNNB1 S45DEL (Ctnnb1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. NgΔIQ FUNgDIQGW

    Plasmid

    #92232

    Purpose

    Lentiviral expression of human Ng with deletion mutation

    Depositor

    Weifeng Xu

    Article

    Hwang et al Biol Psychiatry. 2021 Feb 1;89(3):256-26

    Insert

    Ng (NRGN Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    delta IQ

    Promoter

    hUbC

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-EF1a-ArgiNLS-mGreenLantern

    Plasmid

    #220596

    Purpose

    Constitutive expression of a single-cell discriminating version of mGreenLantern fluorescent protein.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    mGreenLantern
    

    Use

    AAV

    Tags

    ArgiNLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. BLBP-mCherry

    Plasmid

    #63721

    Purpose

    Expressing mCherry from BLBP promoter which is a neural stem cell (radial glial cells) specific promoter

    Depositor

    Bart De Strooper

    Article

    Shariati et al J Cell Sci. 2013 Mar 1;126(Pt 5):1268-77

    Insert

    BLBP (Fabp7 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    1700 bp upstream of BLBP gene cloned in CAG-mCher…

    Promoter

    1700bp upstream of BLBP gene

    Available since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLJM1 FLCN (WT) FLAG GFP

    Plasmid

    #164978

    Purpose

    Lentiviral vector for FLCN expression in mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

    Insert

    FLCN (FLCN Human)
    

    Use

    Lentiviral

    Tags

    1xFLAG-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO.1-sh-hSOX9-1

    Plasmid

    #40644

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    sh-hSOX9-1 (SOX9 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-myc-ERK2-MEK1_fusion

    Plasmid

    #39194

    Depositor

    Melanie Cobb

    Article

    Robinson et al Curr Biol. 1998 Oct 22;8(21):1141-50.

    Insert

    ERK2-MEK1 Fusion (MAP2K1, Mapk1 Rat, Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pCMV-myc-ERK2-L4A-MEK1_fusion

    Plasmid

    #39197

    Depositor

    Melanie Cobb

    Article

    Robinson et al Curr Biol. 1998 Oct 22;8(21):1141-50.

    Insert

    ERK2-MEK1 Fusion (MAP2K1, Mapk1 Rat, Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    L4A = L33A, L37A, L40A, L42A

    Promoter

    CMV

    Available since

    July 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOPINB-hRNF216(511-784)

    Plasmid

    #171923

    Purpose

    Expression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells. Catalytic RBR-helix construct suitable for activity assays.

    Depositor

    Bernhard Lechtenberg

    Article

    Cotton et al Mol Cell. 2022 Feb 3;82(3):598-615.e8. d

    Insert

    E3 ubiquitin-protein ligase RNF216 (RNF216 Human)
    

    Use

    Tags

    3C protease cleavage site and 6x-His tag

    Expression

    Bacterial

    Mutation

    codon optimised for expression in E. coli

    Promoter

    T7

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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