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pET28-PKP3(305-797)

Plasmid

#202599

Purpose

Expresses ARM domain of plakophilin3

Depositor

Tina Izard

Article

Gupta et al Int J Mol Sci. 2023 May 29;24(11):9458.

Insert

plakophilin3 (PKP3 Human)

Use

Tags

His8 tag with precision protease cleavage site

Expression

Bacterial

Mutation

Deleted amino acids 1 to 304

Promoter

Available since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-NEDD4L_W2-W3-W4-HECT (381)

Plasmid

#187708

Purpose

Express his-tagged NEDD4L W2-W3-W4-HECT in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 381-975 (NEDD4L Human)

Use

Tags

His-HRV 3C site

Expression

Bacterial

Mutation

deletion of residues 1-380

Promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-NEDD4L_W3-W4-HECT (492)

Plasmid

#187709

Purpose

Express his-tagged NEDD4L W3-W4-HECT in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 492-975 (NEDD4L Human)

Use

Tags

His-HRV 3C site

Expression

Bacterial

Mutation

deletion of residues 1-491

Promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-a(+)-6xHis-miRFP(-5)-HO1

Plasmid

#219424

Purpose

Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

Depositor

Allie Obermeyer

Article

Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

Inserts

miRFP682
HO1

Use

Tags

MGHHHHHGG

Expression

Bacterial

Mutation

Contains Ribosomal Binding Site for expression of…

Promoter

T7

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pET28b-ptetO::bac::gfp

Plasmid

#217882

Purpose

expresses the bac-BGC in bacterial cells, produces bacillamide D

Depositor

Tobias A. M. Gulder

Article

Hohmann et al Commun Biol. 2024 May 28;7(1):655. doi:

Inserts

Dehydrogenase
Non-ribosomal peptide synthetase
Aminotransferase

Use

Tags

Expression

Bacterial

Mutation

Promoter

ptetO

Available since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-FKBP-IRSp53(pLxIS)

Plasmid

#221336

Purpose

Overexpression of IRSp53 pLxIS domain in bacteria

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

IRSp53 pLxIS domain (Baiap2 Mouse)

Use

Tags

6xHis-4xFKBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-FKBP-MAVS(pLxIS)

Plasmid

#221335

Purpose

Overexpression of MAVS pLxIS domain in bacteria

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

MAVS pLxIS domain (Mavs Mouse)

Use

Tags

6xHis-4xFKBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-FKBP-TRIF(Nterm)

Plasmid

#221334

Purpose

Overexpression of TRIF Nterm domain in bacteria

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TRIF Nterm domain (Ticam1 Mouse)

Use

Tags

6xHis-4xFKBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-FKBP-GMIP(pLxIS)

Plasmid

#221337

Purpose

Overexpression of GMIP pLxIS domain in bacteria

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

GMIP pLxIS domain (Gmip Mouse)

Use

Tags

6xHis-4xFKBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-SnoopTag-AffiHER2-SpyTag

Plasmid

#216280

Purpose

Expresses an affibody to HER2 fused to SnoopTag and SpyTag, for bacterial expression

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

SnoopTag-AffiHER2-SpyTag

Use

Affinity Reagent/ Antibody

Tags

His6, SnoopTag, SpyTag, and Thrombin cleavage sit…

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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